Analysis of plasma exosome containing micro RNA in gastrointestinal cancer

• Project/Area Number: 24590944
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Kyushu University
• Principal Investigator: BABA Eishi 九州大学, 医学(系)研究科(研究院), 教授 (00315475)
• Co-Investigator(Kenkyū-buntansha): NIIRO Hiroaki 九州大学, 大学病院, 准教授 (20380636) ; AKASHI Koichi 九州大学, 大学院医学研究院, 教授 (80380385)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 消化器がん / がん幹細胞 / エクソゾーム / 微小環境 / 可溶性因子 / 消化器癌 / 癌幹細胞

Outline of Final Research Achievements

Human gastrointestinal tumor has cancer stem cell (CSC) population, which possess self-renewing potential. The present study revealed the stemness in the EpCAM-high and CD44-positive human colorectal cancer cells. Only these cells efficiently form sphere in vitro. Soluble factors including exosomes may play an important role in the interaction between colorectal CSCs and the cells forming microenvironment. The present study suggested that these interactions might possibly induce of stemness in non-CSCs.

Research Products

• [Journal Article] CIN85 is required for Cbl-mediated regulation of antigen receptor signaling in human B cells (2012)
  • Author(s): Niiro H,…, Tsukamoto H, et al(16名中12番目)
  • Journal Title: Blood Volume: 119 Issue: 10 Pages: 2263-2273
  • DOI: 10.1182/blood-2011-04-351965
  • Peer Reviewed
• [Presentation] Placiticity of CD44+ colorectal cancer stem cells depend on TGF-beta-induced epithelial mesenchymal transition：evidences from an ex vivo culture (2015)
  • Author(s): Michitaka Nakano, Hiroshi Ariyama, Shingo Tamura, Taichi Isobe, Kohta Miyawaki, Hitoshi Kusaba, Takashi Ueki, Eishi Baba, Koichi Akashi
  • Organizer: American Association for Cancer Research (AACR) 2015
  • Place of Presentation: Philadelphia
  • Year and Date: 2015-04-20
• [Presentation] Placiticity of CD44+ colorectal cancer stem cells depend on TGF-beta-induced epithelial mesenchymal transition：evidences from an ex vivo culture (2014)
  • Author(s): 中野倫孝,有山寛、田村真吾、磯部大地、宮脇恒太、奥村祐太、草場仁志、植木隆、馬場英司、赤司浩一
  • Organizer: 第73回日本癌学会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Placiticity of CD44+ colorectal cancer stem cells depend on TGF-beta-induced epithelial mesenchymal transition：evidences from an ex vivo culture (2014)
  • Author(s): 中野倫孝,有山寛、田村真吾、磯部大地、宮脇恒太、奥村祐太、草場仁志、植木隆、馬場英司、赤司浩一
  • Organizer: 第12回日本臨床腫瘍学会
  • Place of Presentation: 福岡
  • Year and Date: 2014-07-17 – 2014-07-19
• [Presentation] Placiticity of CD44+ colorectal cancer stem cells depend on TGF-beta-induced epithelial mesenchymal transition：evidences from an ex vivo culture (2014)
  • Author(s): Michitaka Nakano, Hiroshi Ariyama, Shingo Tamura, Taichi Isobe, Kohta Miyawaki, Hitoshi Kusaba, Takashi Ueki, Eishi Baba, Koichi Akashi
  • Organizer: American Association for Cancer Research (AACR) 2014
  • Place of Presentation: Dan Diego
  • Year and Date: 2014-04-07
• [Presentation] Plasticity of CD44+ colorectal cancer stem cells depends on TGF-beta-induced epithelial mesenchymal transition (EMT): evidences from ex vivo culture system (2014)
  • Author(s): Michitaka Nakano, Hiroshi Ariyama, Eishi Baba et al.
  • Organizer: American Association for Cancer Research
  • Place of Presentation: San Diego, USA
• [Presentation] Both E-Cadherin-positive and negative cells of colorectal cancer stem cell population possess tumor initiating potential. (2012)
  • Author(s): Tamura S, Isobe T, Baba E, Nakano M, Komoda M, Takaishi S, Kusaba H, Ueki T, Akashi K.
  • Organizer: The 71st Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Sapporo
• [Book] 日本臨床増刊 (2014)
  • Author(s): 中野倫孝、馬場英司、赤司浩一
  • Total Pages: 4
  • Publisher: 日本臨床社
• [Remarks] 九州大学医学部病態修復内科学
  • URL: http://www.1nai.med.kyushu-u.ac.jp