| Project/Area Number |
24590950
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Osaka City University |
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Principal Investigator |
TOMINAGA Kazunari 大阪市立大学, 医学(系)研究科(研究院), 准教授 (80336768)
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| Co-Investigator(Kenkyū-buntansha) |
ARAKAWA Tetsuo 大阪市立大学, 大学院医学研究科, 教授 (60145779)
FUJIWARA Yasuhiro 大阪市立大学, 大学院医学研究科, 准教授 (40285292)
WATANABE Toshio 大阪市立大学, 大学院医学研究科, 准教授 (50336773)
TANIGAWA Tetsuya 大阪市立大学, 大学院医学研究科, 講師 (70423879)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 腸管グリア細胞 / 腸管収縮反応 / 知覚過敏 / 過敏性腸症候群 / ストレス / グリア細胞 / 大腸収縮運動 / 刺激過敏性 / 腸管運動 / 腸管神経系 / ストレス環境 / 腸管過敏性 |
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| Outline of Final Research Achievements |
Male Wistar rats underwent 3-h maternal separation (a chronic stress) during postnatal days 2-14 and 4-h water-immersion stress (an acute stress) in adulthood (at 8 weeks). Glial fibrillary acidic protein (GFAP) expression and the number of enteric neurons were unchanged by these stresses. The density of GFAP-positive EGC processes overlapped with the neurons and the extent of bulbous swelling of terminals increased according to the stress intensity: control, acute stress, maternal separation, and combination. Electrical field stimulation (EFS)-induced colonic contractions were significantly greater in the combined stress rats than in controls. Colonic EGCs exhibited structural alterations according to the stress intensity and associated with stress-induced colonic hyper-contraction, which may underlie the pathogenesis of irritable bowel syndrome.
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