| Project/Area Number |
24590954
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Gastroenterology
|
|---|
| Research Institution | Gunma University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
柿崎 暁 群馬大学, 大学院医学系研究科, 講師 (80344935)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
YOSHINARI Kouichi 静岡県立大学, 大学院薬学系研究科, 教授 (60343399)
|
|---|
| Research Collaborator |
TOJIMA Hiroki 群馬大学, 大学院医学系研究科, 大学院生
TAHARA Hiroki 群馬大学, 大学院医学系研究科, 大学院生
|
|---|
| Project Period (FY) |
2012-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | 核内受容体CAR / オーバル細胞増殖 / 肝前駆細胞 / 肝再生 |
|---|
| Outline of Final Research Achievements |
We have demonstrated that choline-deficient DL-ethionine supplemented (CDE) diet activates the nuclear receptor constitutive active/androstane receptor (CAR), resulting in proliferation of oval cells in mouse liver. Activation of CAR by CDE was shown by cytochrome P450 (CYP)2B10 and DNA damage-induced protein 45 family (GADD45)β mRNA induction after feeding a CDE diet to Car+/+ mice.Unlike 3,5-diethoxycarbonyl-1,4-dihydrocollidine containing diet, the liver damage, and oval cell proliferation were found in both Car+/+ and Car-/- mice after feeding a CDE diet.There was no significant difference of the EpCAM positive cells containing bile duct epithelial cells and liver progenitor cells between the Car+/+ and Car-/- mice as a result of immunostaining analysis
|
