Investigation for biomarker of NASH by comprehensive analysis using peptidomics

• Project/Area Number: 24590969
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Mie University
• Principal Investigator: YAMAMOTO NORIHIKO 三重大学, 医学部附属病院, 講師 (60501963)
• Co-Investigator(Kenkyū-buntansha): SHIRAKI Katsuya 三重大学, 医学系研究科, 准教授 (90263003) ; SUGIMOTO Kazushi 三重大学, 医学部付属病院, 講師 (60378370)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: NASH / バイオマーカー / プロテオミクス / iTAQ法 / 質量分析 / iTRAQ ラベル法 / NASH バイオマーカー

Outline of Final Research Achievements

Recently, the increasing number of metabolic syndrome has become emerging health problem in our country.
As mechanism of this disease has been evaluated, it has become obvious that hepatic steatosis plays an important role in triggering and progression of the disease. Especially, non-alcoholic steatohepatitis(NASH) is very important in the pathogenesis of the disease. But the detailed role of hepatic steatosis in metabolic syndrome remains unclear. Furthermore, it is still very difficult to distinguish simple steatosis from NASH. In this study, we analyzed the blood sample from the healthy volunteer and the NAFLD patients who developed NASH and ultimately liver cirrhosis. We detected the NASH specific molecular peptide by peptidomics using iTRAQ.