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Continuous hepatocyte apoptosis induces HCC in the liver

Research Project

Project/Area Number 24590970
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Gastroenterology
Research InstitutionOsaka University

Principal Investigator

HIKITA Hayato  大阪大学, 医学(系)研究科(研究院), 特任助教(常勤) (20623044)

Co-Investigator(Kenkyū-buntansha) TATSUMI Tomohide  大阪大学, 大学院医学系研究科, 助教 (20397699)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords肝細胞死 / 肝細胞癌 / 酸化ストレス
Outline of Final Research Achievements

Activation of the mitochondrial pathway of apoptosis increased oxidative stress in hepatocytes and tBid released ROS from mitochondria. Hepatocyte-specific Bcl-xL or Mcl-1 KO mice coursed continuous hepatocyte apoptosis with increase of reciprocal regeneration, fibrosis, inflammation cytokines and oxidative stress. They also developed liver tumorgenesis. Bak, Bax or Bid deficiency attenuated hepatocytes apoptosis, oxidative stress and tumorigenesis rate in Mcl-1KO mice. An antioxidant did not affect the levels of hepatocyte apoptosis, reciprocal regeneration, fibrosis and inflammation cytokines but attenuated oxidative stress and decreased incidence rates of HCC. These results indicated that continuous hepatocyte apoptosis induced accumulation of oxidative stress in their liver which resulted in liver carcinogenesis. This study supports a concept that antioxidant therapy may be useful for suppressing liver carcinogenesis in patients with chronic liver disease.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (20 results)

All 2015 2014 2013 2012

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (16 results) (of which Invited: 1 results) Book (1 results)

  • [Journal Article] The Bcl-2 homology 3 (BH3)-only proteins Bim and Bid are functionally active and restrained by anti-apoptotic B-cell CLL/lymphoma 2 (Bcl-2) family proteins in healthy liver.2013

    • Author(s)
      [1] Kodama T, Hikita H, Kawaguchi T, Saito Y, Tanaka S, Shigekawa M, Shimizu S, Li W, Miyagi T, Kanto T, Hiramatsu N, Tatsumi T, Takehara T.
    • Journal Title

      J. Biol. Chem.

      Volume: 288 Issue: 42 Pages: 30009-30018

    • DOI

      10.1074/jbc.m112.443093

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Bak deficiency inhibits liver carcinogenesis : A causal link between apoptosis and carcinogenesis2012

    • Author(s)
      Hikita H, Takehara T, et al
    • Journal Title

      Journal of Hepatology

      Volume: (印刷中) Issue: 1 Pages: 92-100

    • DOI

      10.1016/j.jhep.2012.01.027

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 肝細胞アポトーシスの持続は肝発癌を誘導する2012

    • Author(s)
      疋田隼人, 竹原徹郎.
    • Journal Title

      消化器内科

      Volume: 54 Pages: 734-738

    • Related Report
      2012 Research-status Report
  • [Presentation] Mice with spontaneous hepatocyte apoptosis increased oxidative stress leading to carcinogenesis in the liver2015

    • Author(s)
      Hayato Hikita, Tetuo Takehara
    • Organizer
      The 3rd JSGE International Topic Conference
    • Place of Presentation
      Sendai, Japan
    • Year and Date
      2015-04-24
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Activation of mitochondrial pathway of apoptosis in hepatocytes increases oxidative stress in hepatocytes leading to liver tumorigenesis2014

    • Author(s)
      Hayato Hikita, Tomohide Tatsumi, Yoshinobu Saito, Satoshi Tanaka, Ryotaro Sakamori, Takuya Miyagi, Naoki Hiramatsu, Tetsuo Takehara.
    • Organizer
      The 65th Annual Meeting of the American Association for the study of Liver Disease
    • Place of Presentation
      Boston, USA
    • Year and Date
      2014-11-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] Activation of mitochondrial apoptotic pathway increases oxidative stress in hepatocytes leading to liver carcinogenesis2014

    • Author(s)
      Hayato Hikita, Tomohide Tatsumi, Yoshinobu Saito, Satoshi Tanaka, Minoru Shigekawa, Yoshito Hayashi, Ryotaro Sakamori, Takuya Miyagi, Tetsuo Takehara
    • Organizer
      日本癌学会第73回総会
    • Place of Presentation
      横浜
    • Year and Date
      2014-09-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] 肝障害における金属元素の役割」「肝細胞アポトーシスが惹起するミトコンドリア障害は、酸化ストレスを介して肝発癌を誘導する2014

    • Author(s)
      疋田隼人 巽智秀 竹原徹郎
    • Organizer
      第50回日本肝臓学会総会
    • Place of Presentation
      東京
    • Year and Date
      2014-05-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Oxidative stress induced by continuous hepatocyte apoptosis drives liver carcinogenesis independently of regeneration and DNA methylation status.2013

    • Author(s)
      Hayato Hikita, Tomohide Tatsumi, Yoshinobu Saito, Satoshi Tanaka, Satoshi Shimizu, Wei Li, Ryotaro Sakamori, Takuya Miyagi, Naoki Hiramatsu, Tetsuo Takehara.
    • Organizer
      The 64th Annual Meeting of the American Association for the study of Liver Disease.
    • Place of Presentation
      Washington, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] The rheostat regulating hepatocyte apoptosis by BH3-only proteins in developing and adult liver.2013

    • Author(s)
      Hayato Hikita, Yoshinobu Saito, Satoshi Tanaka, Kawaguchi Tsukawa, Satoshi Shimizu, Takahiro Kodama, Ryotaro Sakamori, Takuya Miyagi, Tomohide Tatsumi and Tetsuo Takehara
    • Organizer
      The 20th Annual Meeting of the Japanese Society for the Reserch of Hepatic Cells.
    • Place of Presentation
      Osaka, Japan
    • Related Report
      2013 Research-status Report
  • [Presentation] Continuous apoptosis in hepatocytes induces oxidative stress and leads to liver carcinogenesis.2013

    • Author(s)
      Hayato Hikita, Tomohide Tatsumi, Tasuku Nakabori, Yoshinobu Saito, Tsukasa Kawaguchi, Satoshi Tanaka, Satoshi Shimizu, Ryotaro Sakamori, Takuya Miyagi, Tetsuo Takehara.
    • Organizer
      The 17th International Symposium on Cell of the Hepatic Sinusoid.
    • Place of Presentation
      Osaka, Japan
    • Related Report
      2013 Research-status Report
  • [Presentation] Continuous apoptosis in hepatocytes induces liver tumors with DNA hypermethylation but not p53 or beta-catenin mutation.2013

    • Author(s)
      Hayato Hikita, Tomohide Tatsumi, Yoshinobu Saito, Satoshi Shimizu, Minoru Shigekawa, Yoshito Hayashi, Ryotaro Sakamori, Takuya Miyagi, Masahiko Tsujii, Tetsuo Takehara
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] 肝細胞アポトーシスが発生する酸化ストレスの肝発癌に与える影響2013

    • Author(s)
      疋田隼人、巽智秀、宮城琢也、阪森亮太郎、清水聡、斉藤義修、田中聡司、竹原徹郎
    • Organizer
      第49回肝臓学会総会
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] 慢性肝障害からの肝発癌におけるバイオマーカーの検討2013

    • Author(s)
      疋田隼人、巽智秀、中堀輔、斎藤義修、田中聡司、清水聡、阪森亮太郎、宮城拓也、竹原徹郎
    • Organizer
      第17回日本肝臓学会大会
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] Antioxidant has a therapeutic potential for HCC driven by chronic liver injury.2013

    • Author(s)
      Hikita H, Tanaka S, Saito Y, Kawaguchi T, Shimizu S, Shigekawa M, Kodama T, Li W, Sakamori R, Miyagi T, Kanto T, Hiramatsu N, Tatsumi T, Takehara T
    • Organizer
      The 2nd JSGE International Topic Conference.
    • Place of Presentation
      Kagoshima
    • Related Report
      2012 Research-status Report
  • [Presentation] Oxidative stresses play a major role in cancer development in apoptosis-prone liver.2012

    • Author(s)
      Hayato Hikita, Takahiro Kodama, Satoshi Tanaka, Yoshinobu Saito, Satoshi Shimizu, Minoru Shigekawa, Wei Li, Takuya Miyagi, Tatsuya Kanto, Naoki Hiramatsu, Tomohide Tatsumi,Tetsuo Takehara.
    • Organizer
      The 63rd Annual Meeting of the American Association for the study of Liver Disease.
    • Place of Presentation
      Boston, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] 肝細胞障害が誘導する酸化ストレスと肝発癌2012

    • Author(s)
      疋田隼人, 巽智秀, 竹原徹郎
    • Organizer
      第54回日本消化器病学会総会
    • Place of Presentation
      神戸
    • Related Report
      2012 Research-status Report
  • [Presentation] Oxidative stresses play a major role in cancer development in apoptosis-prone liver.2012

    • Author(s)
      Hayato Hikita, Takahiro Kodama, Satoshi Tanaka, Yoshinobu Saito, Satoshi Shimizu, Minoru Shigekawa, Wei Li, Takuya Miyagi, Tatsuya Kanto, Naoki Hiramatsu, Tomohide Tatsumi,Tetsuo Takehara.
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Research-status Report
  • [Presentation] 肝細胞のアポトーシスによる酸化ストレス発生と肝発癌2012

    • Author(s)
      疋田隼人, 巽智秀, 小玉尚宏, 清水聡, 重川稔, 田中聡司, 法水淳、宮城琢也、竹原徹郎.
    • Organizer
      第47回日本肝臓学会総会.
    • Place of Presentation
      金沢
    • Related Report
      2012 Research-status Report
  • [Presentation] 肝細胞アポトーシスの持続によるマクロファージの活性化と肝発癌.2012

    • Author(s)
      疋田隼人, 巽智秀, 竹原徹郎.
    • Organizer
      第98回日本消化器病学会総会.
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Book] Annual Review消化器 20142014

    • Author(s)
      疋田隼人、 竹原徹郎
    • Publisher
      中外医学社
    • Related Report
      2013 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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