Role of HCV and periostin on the regulation of liver cancer stem cells
Project/Area Number |
24590994
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Gastroenterology
|
Research Institution | Keio University |
Principal Investigator |
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
Keywords | 癌幹細胞 / HCV / Periostin / 肝臓学 |
Outline of Final Research Achievements |
The aims of the study are to investigate the role of HCV virus on the liver cancer stem cell (CSC) biology; and to investigate the role of periostin on the liver carcinogenesis. The novel findings of the manuscript include: 1) CD133+/SP subpopulation is a distinct CSC population in Huh7 cells; 2) transduction of HCV full-length replicon or HCV core protein expands CSC population and augments CSC properties through two different mechanisms including (a) HCV induced expansion of CSC population through potentiation of self-renewal capacity and (b) transition from non-CSC CD133-/SP cells to CSC CD133+/SP cells; 3) HCV-mediated expansion of CSC population and augmentation of CSC properties are mediated by HIF-1α stabilization; 4) periostin KO mice did not develop CCL4-induced liver fibrosis as compared with WT mice; and 5) periostin KO mice did not develop DEN-induced liver tumors.
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Report
(4 results)
Research Products
(1 results)