Identification of risk factors of hepatocarcinogenesis in fatty liver disease through the comprehensive analysis of epigenetic alterations

• Project/Area Number: 24590997
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Kinki University
• Principal Investigator: NISHIDA Naoshi 近畿大学, 医学部, 准教授 (60281755)
• Co-Investigator(Kenkyū-buntansha): HATANO Etsurou 京都大学, 大学院医学研究科, 准教授 (80359801)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 脂肪性肝疾患 / 肝細胞癌 / エピジェネティクス / がん抑制遺伝子 / 非アルコール性脂肪性肝疾患 / エピゲノム / DNAメチル化 / 脂肪肝 / 糖尿病 / 非アルコール性脂肪性肝炎

Outline of Final Research Achievements

We found that oxidative DAN damage (8-OHdG) induced epigenetic alteration in HCC cell lines. In human chronic hepatitis C, number of methylated tumor suppressor genes (TSGs) was associated with time-to hepatocellular carcinoma (HCC) emergence. Through the comprehensive analysis of DNA methylation in virus-negative HCCs, methylation was affected by aging and presence of diabetes mellitus (DM), suggesting that age and DM might contribute to hepatocarcinogenesis through epigenetic mechanism. We analyzed risk factors of fatty liver-related HCC mergence using 8-OHdG level of hepatocyte as a surrogate marker, and found that serum AFP and hepatocyte ballooning were independently associated with 8-OHdG; number of methylated TSGs was significantly higher in livers with high degree of 8-OHdG levels in hepatocyte. These evidences support the idea that serum AFP level and hepatocyte ballooning could be a marker of high-risk group of HCC in patients with fatty liver.

Research Products

• [Journal Article] Heat shock protein A4/Apg-2 is required for stem/progenitor cell maintenance in intestinal inflammation. (2015)
  • Author(s): Adachi T, Sakurai T, Kashida H, Mine H, Hagiwara S, Matsui S, Yoshida K, Nishida N, Watanabe T, Itoh K, Fujita J, Kudo M.
  • Journal Title: Inflammatory Bowel Disease Volume: 21 Issue: 1 Pages: 31-39
  • DOI: 10.1097/mib.0000000000000244
  • Peer Reviewed / Open Access
• [Journal Article] A bridge between multi-omics data and the management of hepatocellular carcinoma. Annals of Translational Medicine (2015)
  • Author(s): Nishida N and Kudo M.
  • Journal Title: Ann Transl Med. Volume: 3 Pages: 1-1
  • Peer Reviewed / Open Access
• [Journal Article] Cold-inducible RNA-binding protein (Cirp) promotes the development of liver cancer. (2015)
  • Author(s): Sakurai T, Yada N, Watanabe T, Arizumi T, Hagiwara S, Ueshima K, Nishida N, Fujita J, Kudo M.
  • Journal Title: Cancer Sci Volume: in press Issue: 4 Pages: 352-358
  • DOI: 10.1111/cas.12611
  • Peer Reviewed / Open Access
• [Journal Article] Comparison of systems for assessment of post-therapeutic response to sorafenib for hepatocellular carcinoma. (2014)
  • Author(s): Arizumi T, Ueshima K, Takeda H, Osaki Y, Hayaishi S, Takita M, Inoue T, Kitai S, Yada N, Hagiwara S, Minami Y, Sakurai T, Nishida N, Kudo M.
  • Journal Title: J Gastroenterol. Volume: 49 Issue: 12 Pages: 1578-1587
  • DOI: 10.1007/s00535-014-0936-0
  • Peer Reviewed
• [Journal Article] Therapeutic Response Assessment of RFA for HCC: Contrast-enhanced US, CT and MRI. (2014)
  • Author(s): Minami Y, Nishida N, Kudo M.
  • Journal Title: World J Gastroentel Volume: 20 Issue: 15 Pages: 4160-4166
  • DOI: 10.3748/wjg.v20.i15.4160
  • Peer Reviewed / Open Access
• [Journal Article] Stress response protein Cirp links inflammation and tumorigenesis in colitis-associated cancer. (2014)
  • Author(s): Sakurai T, Kashida H, Watanabe T, Hagiwara S, Mizushima T, Iijima H, Nishida N, Higashitsuji H, Fujita J, Kudo M.
  • Journal Title: Cancer Res Volume: 74 Issue: 21 Pages: 6119-6128
  • DOI: 10.1158/0008-5472.can-14-0471
  • Peer Reviewed / Open Access
• [Journal Article] Genome-wide profiling of DNA methylation and tumor progression in human hepatocellular carcinoma. (2014)
  • Author(s): Nishida N*, Nishimura T, Nakai T, Chishina H, Arizumi T, Takita M, Kitai S, Yada N, Hagiwara S, Inoue T, Minami Y, Ueshima K, Sakurai T, Kudo M.
  • Journal Title: Dig Dis. Volume: 32 Issue: 6 Pages: 658-663
  • DOI: 10.1159/000367982
  • Peer Reviewed / Open Access
• [Journal Article] Identification of the epigenetically inactivated genes in human hepatocellular carcinoma by integrative analyses of methylation profiling and pharmacological unmasking. (2014)
  • Author(s): Nishida N, Chishina H, Arizumi T, Takita M, Kitai S, Yada N, Hagiwara S, Inoue T, Minami Y, Ueshima K, Sakurai T, Kudo M.
  • Journal Title: Dig Dis. Volume: 32 Issue: 6 Pages: 740-746
  • DOI: 10.1159/000368015
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Clinical features of vascular disorders associated with chronic hepatitis virus infection. (2014)
  • Author(s): Nishida N and Kudo M
  • Journal Title: Dig Dis. Volume: 32 Issue: 6 Pages: 786-790
  • DOI: 10.1159/000368023
  • Peer Reviewed
• [Journal Article] Treatment response and tolerability in eldery patients with chronic hepatitis C: Subgroup analysis in ReGIT-J study. (2014)
  • Author(s): Nishikawa H, Enomoto H, Saito M, Aizawa N, Tsuda Y, Higuchi K, Okazaki K, Seki T, Kim S-R, Hongo Y, Jyomura H, Nishida N, Kudo M, Osaki Y and Nishiguchi S.
  • Journal Title: J Virol Antivir Res Volume: 3 Issue: 02 Pages: 2-2
  • DOI: 10.4172/2324-8955.1000125
  • Peer Reviewed
• [Journal Article] Radiofrequency Ablation for Hepatocellular Carcinoma Measuring 2 cm or Smaller: Results and Risk Factors for Local Recurrence. (2014)
  • Author(s): Kono M, Inoue T, Kudo M, Chishina H, Arizumi T, Takita M, Kitai S, Yada N, Hagiwara S, Minami Y, Ueshima K, Nishida N, Murakami T.
  • Journal Title: Dig Dis. Volume: 32 Issue: 6 Pages: 670-677
  • DOI: 10.1159/000367999
  • Peer Reviewed
• [Journal Article] Duration of stable disease is associated with overall survival in patients with advanced hepatocellular carcinoma treated with sorafenib. (2014)
  • Author(s): Arizumi T, Ueshima K, Chishina H, Kono M, Takita M, Kitai S, Inoue T, Yada N, Hagiwara S, Minami Y, Sakurai T, Nishida N, Kudo M.
  • Journal Title: Dig Dis. Volume: 32 Issue: 6 Pages: 705-710
  • DOI: 10.1159/000368006
  • Peer Reviewed / Open Access
• [Journal Article] Decreased blood flow after sorafenib administration is an imaging biomarker to predict overall survival in patients with advanced hepatocellular carcinoma. (2014)
  • Author(s): Arizumi T, Ueshima K, Chishina H, Kono M, Takita M, Kitai S, Inoue T, Yada N, Hagiwara S, Minami Y, Sakurai T, Nishida N, Kudo M.
  • Journal Title: Dig Dis. Volume: 32 Issue: 6 Pages: 733-739
  • DOI: 10.1159/000368013
  • Peer Reviewed / Open Access
• [Journal Article] Validation of the Criteria of Transcatheter Arterial Chemoembolization Failure or Refractoriness in Patients with Advanced Hepatocellular Carcinoma Proposed by the LCSGJ. (2014)
  • Author(s): Arizumi T, Ueshima K, Chishina H, Kono M, Takita M, Kitai S, Inoue T, Yada N, Hagiwara S, Minami Y, Sakurai T, Nishida N, Kudo M.
  • Journal Title: Oncology Volume: 87 Issue: Suppl. 1 Pages: 32-36
  • DOI: 10.1159/000368143
  • Peer Reviewed / Open Access
• [Journal Article] Combination Guidance of Contrast-Enhanced US and Fusion Imaging in Radiofrequency Ablation for Hepatocellular Carcinoma with Poor Conspicuity on Contrast-Enhanced US/Fusion Imaging. (2014)
  • Author(s): Minami T, Minami Y, Chishina H, Arizumi T, Takita M, Kitai S, Yada N, Inoue T, Hagiwara S, Ueshima K, Nishida N, Kudo M.
  • Journal Title: Oncology Volume: 87 Issue: Suppl. 1 Pages: 55-62
  • DOI: 10.1159/000368146
  • Peer Reviewed
• [Journal Article] Ultrasound elastography correlates treatment response by antiviral therapy in patients with chronic hepatitis C. (2014)
  • Author(s): Yada N, Sakurai T, Minami T, Arizumi T, Takita M, Inoue T, Hagiwara S, Ueshima K, Nishida N, Kudo M.
  • Journal Title: Oncology Volume: 87 Issue: Suppl. 1 Pages: 118-123
  • DOI: 10.1159/000368155
  • Peer Reviewed / Open Access
• [Journal Article] Heat shock protein 27 expression is inversely correlated with atrophic gastritis and intraepithelial neoplasia. (2013)
  • Author(s): Nagata Y, Kudo M, Nagai T, Watanabe T, Kawasaki M, Asakuma Y, Hagiwara S, Nishida N, Matsui S, Kashida H, Sakurai T
  • Journal Title: Dig Dis Sci. Volume: 58 Issue: 2 Pages: 381-388
  • DOI: 10.1007/s10620-012-2342-x
  • Peer Reviewed
• [Journal Article] Recent advancements in comprehensive genetic analyses for human hepatocellular carcinoma. (2013)
  • Author(s): Nishida N and Kudo M
  • Journal Title: Oncology Volume: 84 Issue: Suppl. 1 Pages: 93-97
  • DOI: 10.1159/000345897
  • Peer Reviewed
• [Journal Article] The prognostic value of vascular endothelial growth factor in hepatocellular carcinoma for predicting metastasis after curative resection. (2013)
  • Author(s): Minata M, Harada K, Kudo M, Ikai I, and Nishida N
  • Journal Title: Oncology Volume: 84 Issue: Suppl. 1 Pages: 71-78
  • DOI: 10.1159/000345894
  • Peer Reviewed
• [Journal Article] Expression of E-cadherin and vascular endothelial growth factor in non-cancerous liver is associated with recurrence of HCC after curative resection. (2013)
  • Author(s): Minata M, Kudo M, Harada K, Ikai I, and Nishida N
  • Journal Title: Oncology Volume: 84 Issue: Suppl. 1 Pages: 88-92
  • DOI: 10.1159/000345896
  • Peer Reviewed
• [Journal Article] Quantification of tumor DNA in serum and vascular invasion of human hepatocellular carcinoma. (2013)
  • Author(s): Nishida N, Arizumi T, Takita M, Nagai T, Kitai S, Yada N, Hagiwara S, Inoue T, Minami Y, Ueshima K, Sakurai T et al
  • Journal Title: Oncology Volume: 84 Issue: Suppl. 1 Pages: 82-87
  • DOI: 10.1159/000345895
  • Peer Reviewed
• [Journal Article] Usefulness of contrast-enhanced ultrasonography to evaluate the post-treatment responses of radiofrequency ablation for hepatocellular carcinoma: comparison with dynamic CT. (2013)
  • Author(s): Inoue T, Kudo M, Hatanaka K, Arizumi T, Takita M, Kitai S, Yada N, Hagiwara S, Minami Y, Sakurai T et al
  • Journal Title: Oncology. Volume: 84 Issue: Suppl. 1 Pages: 51-57
  • DOI: 10.1159/000345890
  • Peer Reviewed
• [Journal Article] Impact of Combination Therapy with Peginterferon alpha-2b and Entecavir on Persistent Viral Suppression in Patients with Chronic Hepatitis B. (2013)
  • Author(s): Hagiwara S, Kudo M, Osaki Y, Matsuo H, Inuzuka T, Matsumoto A, Tanaka E, Sakurai, Ueshima K, Inoue T, Yada N, Nishida N
  • Journal Title: J Med Virol. Volume: 85 Issue: 6 Pages: 987-995
  • DOI: 10.1002/jmv.23564
  • Peer Reviewed
• [Journal Article] Association of Gankyrin and stemness factor expression in human colorectal cancer (2013)
  • Author(s): Mine H, Sakurai T, Kashida H, Matsui S, Nishida N, Nagai T, Hagiwara S, Watanabe T, Kudo M.
  • Journal Title: Dig Dis Sci Volume: 58 Issue: 8 Pages: 2337-44
  • DOI: 10.1007/s10620-013-2627-8
  • Peer Reviewed
• [Journal Article] Unique association between global DNA hypomethylation and chromosomal alterations in human hepatocellular carcinoma. (2013)
  • Author(s): Nishida N, Kudo M, Nishimura T, Arizumi T, Takita M, Kitai S, Yada N, Hagiwara S, Inoue T, Minami Y, Ueshima K, Sakurai T, Yokomichi N, Nagasaka T, Goel A.
  • Journal Title: PLoS One Volume: 8 Issue: 9 Pages: e72312-e72312
  • DOI: 10.1371/journal.pone.0072312
  • Peer Reviewed
• [Journal Article] Reactive Oxygen Species Induce Epigenetic Instability through the Formation of 8-Hydroxydeoxyguanosine in Human Hepatocarcinogenesis. (2013)
  • Author(s): Nishida N, Arizumi T, Takita M, Kitai S, Yada N, Hagiwara S, Inoue T, Minami Y, Ueshima K, Sakurai T, Kudo M.
  • Journal Title: Dig Dis. Volume: 31 Issue: 5-6 Pages: 459-466
  • DOI: 10.1159/000355245
  • Peer Reviewed
• [Journal Article] Oxidative stress and epigenetic instability in human hepatocarcinogenesis. (2013)
  • Author(s): Nishida N, Kudo M.
  • Journal Title: Dig Dis. Volume: 31 Issue: 5-6 Pages: 447-453
  • DOI: 10.1159/000355243
  • Peer Reviewed
• [Journal Article] Hypothermia protects against fulminant hepatitis in mice by reducing reactive oxygen species production. (2013)
  • Author(s): Sakurai T, Kudo M, Watanabe T, Itoh K, Higashitsuji H, Arizumi T, Inoue T, Hagiwara S, Ueshima K, Nishida N, Fukumoto M, Fujita J.
  • Journal Title: Dig Dis Volume: 31 Issue: 5-6 Pages: 440-6
  • DOI: 10.1159/000355242
  • Peer Reviewed
• [Journal Article] Clinical and histological features of different types of budd-Chiari syndrome: a comparison of 4 cases. (2013)
  • Author(s): Nishida N, Iwamura S, Ida H, Hagiwara S, Kagioka Y, Minami Y, Maetani Y, Itoh K, Kudo M.
  • Journal Title: Dig Dis. Volume: 31 Issue: 5-6 Pages: 408-414
  • DOI: 10.1159/000355237
  • Peer Reviewed
• [Journal Article] Advanced hepatocellular carcinoma with lymph node metastases showing epithelial to mesenchymal transition effectively treated with systemic chemotherapy: report of a case. (2012)
  • Author(s): Hiroshi Sadamori
  • Journal Title: Hepatology Research Volume: 20 Issue: 12 Pages: 117-121
  • DOI: 10.1111/hepr.12080
  • Peer Reviewed
• [Journal Article] Characteristic patterns of altered DNA methylation predict emergence of human hepatocellular carcinoma (2012)
  • Author(s): Naoshi Nishida
  • Journal Title: Hepatology Volume: 56 Issue: 3 Pages: 994-1003
  • DOI: 10.1002/hep.25706
  • Peer Reviewed
• [Journal Article] Activation of JNK and High Expression Level of CD133 Predict a Poor Response to Sorafenib in Hepatocellular Carcinoma. (2012)
  • Author(s): Hagiwara S
  • Journal Title: Br J Cancer Volume: 106 Pages: 1997-2003
  • Peer Reviewed
• [Journal Article] Risk of hepatocellular carcinoma development in cases of hepatitis C treated by long-term, low-dose PEG-IFNα-2a. (2012)
  • Author(s): Nishida N, Arizumi T, Hayaishi S, Takita M, Kitai S, Yada N, Hagiwara S, Inoue T, Minami Y, Ueshima K, Sakurai T,
  • Journal Title: Dig Dis Volume: 30 Issue: 6 Pages: 547-553
  • DOI: 10.1159/000343057
  • Peer Reviewed
• [Journal Article] Risk of hepatocellular carcinoma development in cases of hepatitis C treated by long-term, low-dose PEG-IFNα-2a. (2012)
  • Author(s): Hagiwara S
  • Journal Title: Dig Dis Volume: 30 Issue: 6 Pages: 561-567
  • DOI: 10.1159/000343065
  • Peer Reviewed
• [Presentation] 肝細胞癌に対してUS-US Fusionを用いたラジオ波焼灼術 (2015)
  • Author(s): 南康範、西田直生志、工藤正俊
  • Organizer: 第101回日本消化器病学会総会 ワークショップ7「Navigationに基づいた肝細胞癌IVR治療の最前線」
  • Place of Presentation: 仙台国際センター（宮城県・仙台市）
  • Year and Date: 2015-04-23 – 2015-04-25
• [Presentation] Drug freeを目指したエンテカビルとPEG-IFNα2a/2b 48週併用療法の効果について (2015)
  • Author(s): 萩原智、西田直生志、工藤正俊
  • Organizer: 第101回日本消化器病学会総会 ワークショップ6「HBV感染におけるDrug freeを目指した基礎と臨床のブレークスルー」
  • Place of Presentation: 仙台国際センター（宮城県・仙台市）
  • Year and Date: 2015-04-23 – 2015-04-25
• [Presentation] Pathological feature, oxidative DNA damage and epigenetic alteration of tumor suppressor genes in non-alcoholic fatty liver disease. (2014)
  • Author(s): Nishida N, Yada N, Arizumi T,Takita M, Kitai S, Inoue T, Hagiwara S,Minami Y, Sakurai T, Ueshima K, Kudo M
  • Organizer: 65th Annual meeting of the American Association for the Study of Liver Diseases (AASLD).
  • Place of Presentation: Boston, MA, USA
  • Year and Date: 2014-11-08 – 2014-11-11
• [Presentation] NBNC肝癌の背景肝におけるメチル化プロファイルと加齢および糖尿病の影響 (2014)
  • Author(s): 西田直生志、工藤正俊
  • Organizer: 第22回日本消化器関連学会週間(第18回日本肝臓学会大会) シンポジウム14「NBNC肝がんの諸問題」
  • Place of Presentation: 神戸国際展示場・ポートピアホテル（兵庫県・神戸市）
  • Year and Date: 2014-10-23 – 2014-10-25
• [Presentation] Oxidative stress and epigenetic instability in human hepatocarcinogenesis (2014)
  • Author(s): Nishida N
  • Organizer: 4th International Kyoto Liver Cancer Symposium. Eraly Morning Breakfast Workshop 3:Carcinogenesis, Genomics, Pathways and Targets
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2014-06-07 – 2014-06-08
  • Invited
• [Presentation] 肝細胞癌(HCC)合併の非代償性肝硬変患者に対する局所治療の有用性についての検討 (2014)
  • Author(s): 北井聡、工藤正俊、西田直生志、泉並木、坂元亨宇、松山裕、市田隆文、中島収、松井修、具英成、國土典宏、幕内雅敏
  • Organizer: 第50回日本肝癌研究会 ワークショップ1「Child-Pugh C肝癌に対する治療」
  • Place of Presentation: 京都国際会議場（京都府・京都市）
  • Year and Date: 2014-06-05 – 2014-06-06
• [Presentation] ヒト肝発癌における酸化ストレスとエピゲノム変異の関連 (2014)
  • Author(s): 萩原智、西田直生志、工藤正俊
  • Organizer: 第50回日本肝癌研究会 パネルディスカッッション2「ゲノム•エピゲノム解析に基づく肝癌診療の将来展望」
  • Place of Presentation: 京都国際会議場（京都府・京都市）
  • Year and Date: 2014-06-05 – 2014-06-06
• [Presentation] 肝細胞癌におけるサイトケラチン19の発現とメチル化の意義 (2014)
  • Author(s): 横道直佑、永坂岳司、西田直生志、久保田暢人、吉田一博、藤原裕子、藤智和、河合毅、谷口文崇、八木孝仁、藤原俊儀
  • Organizer: 第50回日本肝癌研究会 パネルディスカッッション2「ゲノム•エピゲノム解析に基づく肝癌診療の将来展望」
  • Place of Presentation: 京都国際会議場（京都府・京都市）
  • Year and Date: 2014-06-05 – 2014-06-06
• [Presentation] NASH/NAFLDモデルマウスの発癌過程における酸化的DNA損傷とエピゲノム変異の誘導 (2014)
  • Author(s): 西田直生志、工藤正俊
  • Organizer: 第100回日本消化器病学会総会 ワークショップ9: NAFLD/NASHにおける新知見と治療法の進歩
  • Place of Presentation: 東京国際フォーラム（東京都・千代田区）
  • Year and Date: 2014-04-23 – 2014-04-26
• [Presentation] Serum microRNAs as diagnostic biomarkers for early colorectal neoplasms. (2014)
  • Author(s): Yamada A, Horimatsu T, Okugawa Y, Nishida N, Kou T, Kusaka T, Honjo H, Amanuma Y, Kikuchi O, Muto M, Goel A, Boland CR.
  • Organizer: Annual meeting of the American Association for cancer research (AACR) 2014
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2014-04-05 – 2014-04-09
• [Presentation] Role of oxidative stress and epigenetic alteration on chronic hepatitis C-related human hepatocarcinogenesis. (2013)
  • Author(s): Naoshi Nishida
  • Organizer: 64th Annual meeting of the American Association for the Study of Liver Diseases (AASLD)
  • Place of Presentation: Convention Center, Washington, D.C
• [Presentation] Unique association between global DNA hypomethylation and chromosomal alterations in human hepatocellular carcinoma. (2013)
  • Author(s): Naoshi Nishida
  • Organizer: 7th ILCA (International Liver Cancer Association) Annual Conference
  • Place of Presentation: Convention Center, Washington, D.C
• [Presentation] ヒト肝癌に認められるゲノムワイドな脱メチル化と染色体不安定性の関連 (2013)
  • Author(s): 西田直生志
  • Organizer: 第49回日本肝臓学会総会
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
• [Presentation] Risk Assessment of Hepatocellular Carcinoma Using Epigenetic Alterations in Chronic Hepatitis (2012)
  • Author(s): Naoshi Nishida
  • Organizer: The 9th Korea-Japan Liver Symposium
  • Place of Presentation: Seoul, Korea
  • Invited
• [Presentation] Novel Association between global DNA hypomethylation and chromosomal instability phenotype in human hepatocellular carcinoma (2012)
  • Author(s): Naoshi Nishida
  • Organizer: 82th Digestive Disease Week (DDW):AASLD Basic Plenary
  • Place of Presentation: SanDiego, CA, USA
• [Presentation] 肝発癌における酸化ストレスとエピゲノム変異 (2012)
  • Author(s): 西田直生志
  • Organizer: 第20回日本消化器関連学会週間(第16回日本肝臓学会大会)
  • Place of Presentation: 神戸
• [Presentation] Impact of DNA methylation alterations in chronic hepatitis C on emergence of human hepatocellular carcinoma (2012)
  • Author(s): Naoshi Nishida
  • Organizer: 第71回日本癌学会学術総会: English Oral Session (DNA methylation and Biomarker)
  • Place of Presentation: 札幌
• [Presentation] Genome-wide methylation profiling of human hepatocellular carcinoma by using BeadChip 450K. (2012)
  • Author(s): Takafumi Nishimura
  • Organizer: 第71回日本癌学会学術総会: English Oral Session (Epigenetics: DNA methylome)
  • Place of Presentation: 札幌
• [Presentation] Cytokeratin 19 predicts extrahepatic metastasis for hepatocellular carcinoma with epitherial-mesenchimal transition. (2012)
  • Author(s): Naosuke Yokomichi
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Book] 肝癌におけるDNAメチル化プロファイリングの現状と意義, 肝胆膵 特集-肝胆膵腫瘍におけるバイオインフォマティクス (2014)
  • Author(s): 西田直生志、工藤正俊
  • Total Pages: 11
  • Publisher: アークメディア
• [Remarks] 近畿大学医学部消化器内科研究内容
  • URL: http://www.med.kindai.ac.jp/shoukaki/research/index.html
• [Remarks] 研究業績2014
  • URL: http://www.med.kindai.ac.jp/shoukaki/research/2014.pdf
• [Remarks] 消化器内科研究内容
  • URL: http://www.med.kindai.ac.jp/shoukaki//research/index.html
• [Remarks] 消化器内科研究内容
  • URL: http://www.med.kindai.ac.jp/shoukaki//research/index.html