Elucidation of the biological function of non-coding repeat RNA in pancreatic cancer using mouse models

• Project/Area Number: 24591005
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: The University of Tokyo
• Principal Investigator: YAMAMOTO Natsuyo 東京大学, 医学部附属病院, 助教 (40599578)
• Co-Investigator(Kenkyū-buntansha): OTSUKA Motoyuki 東京大学, 医学部附属病院消化器内科, 助教 (90518945) ; IJICHI Hideaki 東京大学, 医学部附属病院消化器内科, 助教 (70463841)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 膵癌 / 非コードRNA / 染色体分裂異常

Outline of Final Research Achievements

Repeat RNA, one of the non-coding RNAs which express in pancreatic cancer tissues, is expressed exclusively in the cancerous tissues. Its expression was not detected in the normal pancreas tissues. Thus, we hypothesized that this repeat RNA may be involved in the parthenogenesis of the pancreatic cancer. To clarify this, we constructed onverexpression system of this repeat RNA in culture cells. We observed deregulated chromatin division and impaired repair of the damaged DNA in cells, which may contribute to the malignant phenotype of cancer cells.

Research Products

• [Journal Article] Risk factors for post-ERCP pancreatitis in wire-guided cannulation for therapeutic biliary ERCP. (2015)
  • Author(s): Nakai Y, Isayama H, Sasahira N, Kogure H, Sasaki T, Yamamoto N, Saito K, Umefune G, Akiyama D, Kawahata S, Matsukawa M, Saito T, Hamada T, Takahara N, Mizuno S, Miyabayashi K, Mohri D, Hirano K, Tada M, Koike K.
  • Journal Title: Gastrointest Endosc. Volume: 81 Issue: 1 Pages: 119-26
  • DOI: 10.1016/j.gie.2014.06.005
  • Peer Reviewed
• [Journal Article] Endoscopic minor papilla balloon dilation for the treatment of symptomatic pancreas divisum. (2014)
  • Author(s): Yamamoto N, Isayama H, Sasahira N, Tsujino T, Nakai Y, Miyabayashi K, Mizuno S, Kogure H, Sasaki T, Hirano K, Tada M, Koike K.
  • Journal Title: Pancreas. Volume: 43 Issue: 6 Pages: 927-30
  • DOI: 10.1097/mpa.0000000000000148
  • Peer Reviewed
• [Journal Article] Transmural biliary drainage can be an alternative to transpapillary drainage in patients with an indwelling duodenal stent. (2014)
  • Author(s): Hamada T, Isayama H, Nakai Y, Kogure H, Yamamoto N, Kawakubo K, Takahara N, Uchino R, Mizuno S, Sasaki T, Togawa O, Matsubara S, Ito Y, Hirano K, Tsujino T, Tada M, Koike K.
  • Journal Title: Dig Dis Sci. Volume: 59 Issue: 8 Pages: 1931-8
  • DOI: 10.1007/s10620-014-3062-1
  • Peer Reviewed
• [Journal Article] Prognostic nomogram for nonresectable pancreatic cancer treated with gemcitabine-based chemotherapy (2014)
  • Author(s): Hamada T(1), Nakai Y(1), Yasunaga H(2), Isayama H, Matsui H, Takahara N, Sasaki T, Takagi K, Watanabe T, Yagioka H, Kogure H, Arizumi T, Yamamoto N, Ito Y, Hirano K, Tsujino T, Tada M, Koike K.
  • Journal Title: Br J Cancer Volume: in press Issue: 8 Pages: 1943-9
  • DOI: 10.1038/bjc.2014.131
  • Peer Reviewed
• [Journal Article] Slow Pull Versus Suction in Endoscopic Ultrasound-Guided Fine-Needle Aspiration of Pancreatic Solid Masses (2014)
  • Author(s): Nakai Y, Isayama H, Chang KJ, Yamamoto N, Hamada T, Uchino R, Mizuno S, Miyabayashi K, Yamamoto K, Kawakubo K, Kogure H, Sasaki T, Hirano K, Tanaka M, Tada M, Fukayama M, Koike K.
  • Journal Title: Dig Dis Sci. Volume: in press
  • Peer Reviewed
• [Journal Article] A retrospective study of S-1 and oxaliplatin combination chemotherapy in patients with refractory pancreatic cancer (2013)
  • Author(s): Takahara N, Isayama H, Nakai Y, Sasaki T, Hamada T, Uchino R, Mizuno S, Miyabayashi K, Kogure H, Yamamoto N, Sasahira N, Hirano K, Ijichi H, Tateishi K, Tada M, Koike K.
  • Journal Title: Cancer Chemother Pharmacol. Volume: 72(5) Issue: 5 Pages: 985-90
  • DOI: 10.1007/s00280-013-2278-7
  • Peer Reviewed
• [Journal Article] Diabetes is a useful diagnostic clue to improve the prognosis of pancreatic cancer (2013)
  • Author(s): Mizuno S, Nakai Y, Isayama H, Takahara N, Miyabayashi K, Yamamoto K, Kawakubo K, Mohri D, Kogure H, Sasaki T, Yamamoto N, Sasahira N, Hirano K, Tsujino T, Ijichi H, Tateishi K, Tada M, Koike K
  • Journal Title: Pancreatology Volume: 13(3) Issue: 3 Pages: 285-9
  • DOI: 10.1016/j.pan.2013.03.013
  • Peer Reviewed
• [Journal Article] Preliminary report on a new, fully covered, metal stent designed for the treatment of pancreatic fluid collections. (2013)
  • Author(s): Yamamoto N(1), Isayama H, Kawakami H, Sasahira N, Hamada T, Ito Y, Takahara N, Uchino R, Miyabayashi K, Mizuno S, Kogure H, Sasaki T, Nakai Y, Kuwatani M, Hirano K, Tada M, Koike K.
  • Journal Title: Gastrointest Endosc. Volume: 77(5) Issue: 5 Pages: 809-14
  • DOI: 10.1016/j.gie.2013.01.009
  • Peer Reviewed
• [Journal Article] High single-session success rate of endoscopic bilateral stent-in-stent placement with modified large cell Niti-S stents for malignant hilar biliary obstruction. (2013)
  • Author(s): Yamamoto N et al. (19人中15番目)
  • Journal Title: Dig Endosc. Volume: Epub Issue: 1 Pages: 93-99
  • DOI: 10.1111/den.12055
  • Peer Reviewed
• [Journal Article] Uridine diphosphate glucuronosyl transferase 1 family polypeptide A1 gene (UGT1A1) polymorphisms are associated with toxicity and efficacy in irinotecan monotherapy for refractory pancreatic cancer (2013)
  • Author(s): Takahara N, Nakai Y, Isayama H, Sasaki T, Satoh Y, Takai D, Miyabayashi K, Mohri D, Kawakubo K, Kogure H, Yamamoto N, Sasahira N, Hirano K, Ijichi H, Tada M, Yatomi Y, Koike K
  • Journal Title: Cancer Chemotherapy and Pharmacology Volume: 71 Issue: 1 Pages: 85-92
  • DOI: 10.1007/s00280-012-1981-0
  • Peer Reviewed
• [Journal Article] High alcohol consumption increases the risk of pancreatic stone formation and pancreatic atrophy in autoimmune pancreatitis. (2012)
  • Author(s): Yamamoto et al (20人中13番目)
  • Journal Title: Pancreas. Volume: 42 Issue: 3 Pages: 502-5
  • DOI: 10.1097/mpa.0b013e31826b3984
  • Peer Reviewed
• [Journal Article] Risk factors and early signs of pancreatic cancer in diabetes: screening strategy based on diabetes onset age. (2012)
  • Author(s): Yamamoto et al. (20人中12番目)
  • Journal Title: J Gastroenterol. Volume: 48 Issue: 2 Pages: 238-46
  • DOI: 10.1007/s00535-012-0622-z
  • NAID: 10031156309
  • Peer Reviewed
• [Remarks] 研究グループホームページ
  • URL: https://sites.google.com/site/225kenncrna/
• [Remarks] 東京大学胆膵グループホームページ
  • URL: http://todai-tansui.com/