O-glycosylation by GalNAc-T3 in pancreatic cancer

• Project/Area Number: 24591013
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Kochi University
• Principal Investigator: TANIUCHI Keisuke 高知大学, 医学部附属病院, 特任准教授 (50626869)
• Co-Investigator(Renkei-kenkyūsha): HONKE Koichi 高知大学, 教育研究部医療学系, 教授 (80190263) ; KOTANI Norihiro 埼玉医科大学, 医学部・生化学講座, 准教授 (90342782)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 膵臓学 / O結合型糖鎖 / 膵臓がん / 細胞増殖

Outline of Final Research Achievements

This project seeks to identify the formation of uncommon structure of glycoforms catalyzed by one of GalNAc-transferases GalNAcT-3 on the target glycoprotein GNAT1. The data provided here could provide important information to aid to improve the clinical outcome of pancreatic cancer patients. We determined the O-glycosylation sites and carbohydrate alterations on GNAT1 in pancreatic cancer cells. Furthermore, GNAT1-bound proteins were identified and these complexes were associated with cell proliferation in pancreatic cancer cells. These discovery based studies will provide insight into the manner in which GalNAcT-3 acts on the specific substrate proteins that are important to growth/survival of pancreatic cancer cells and contributes to disease process in epigenetic manner (formation of uncommon structure of glycoforms) that is based on its substrate specificities.

Research Products

• [Journal Article] Prdx1 promotes pancreatic cancer cell invasion by modulating p38 MAPK activity. (2015)
  • Author(s): Taniuchi K, Furihata M, Hanazaki K, Iwasaki S, Tanaka K, Shimizu T, Saito M, Saibara T
  • Journal Title: Pancreas Volume: 44 Issue: 2 Pages: 331-340
  • DOI: 10.1097/mpa.0000000000000270
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] KIF20Aと結合するRNA結合蛋白質由来ペプチドワクチンの新規膵癌治療への応用 (2015)
  • Author(s): 谷内恵介
  • Journal Title: がん治療のあゆみ Volume: 34 Pages: 34-39
• [Journal Article] Possible inhibitory role of endogenous 2-arachidonoylglycerol as an endocannabinoid in (±)-epibatidine-induced activation of central adrenomedullary outflow in the rat. (2015)
  • Author(s): Shimizu T, Tanaka K, Shimizu S, Higashi Y, Yawata T, Nakamura K, Taniuchi K, Ueba T, Yuri K, Saito M
  • Journal Title: Neuropharmacology Volume: 95 Pages: 278-289
  • DOI: 10.1016/j.neuropharm.2015.03.034
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effect of an angiotensin II receptor blocker and a calcium channel blocker on hypertension associated penile dysfunction in a rat model. (2014)
  • Author(s): Shimizu S, Tsounapi P, Honda M, Dimitriadis F, Taniuchi K, Shimizu T, Inoue K, Saito M
  • Journal Title: Biomed Res Volume: 35 Pages: 215-221
  • NAID: 130004470870
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] IGF2BP3-mediated translation in cell protrusions promotes cell invasiveness and metastasis of pancreatic cancer. (2014)
  • Author(s): Taniuchi K, Furihata M, Hanazaki K, Saito M, Saibara T
  • Journal Title: Oncotarget Volume: 5 Pages: 6832-6845
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] KIF20A-mediated RNA granule transport system promotes the invasiveness of pancreatic cancer cells. (2014)
  • Author(s): Taniuchi K, Furihata M, Saibara T
  • Journal Title: Neoplasia Volume: 16 Issue: 12 Pages: 1082-1093
  • DOI: 10.1016/j.neo.2014.10.007
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Angiotensin II acting on brain AT1 receptors induces adrenaline secretion and pressor responses in the rat. (2014)
  • Author(s): Nakamura K, Shimizu T, Yanagita T, Nemoto T, Taniuchi K, Shimizu S, Dimitriadis F, Yawata T, Higashi Y, Ueba T, Saito, M
  • Journal Title: Sci Rep Volume: 4 Issue: 1 Pages: 7248-7248
  • DOI: 10.1038/srep07248
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Brain RVD-haemopressin, a haemoglobin-derived peptide, inhibits bombesin-induced central activation of adrenomedullary outflow in the rat (2014)
  • Author(s): Tanaka K, Shimizu T, Yanagita T, Nemoto T, Nakamura K, Taniuchi K, Dimitriadis F, Yokotani K, Saito M
  • Journal Title: Br. J. Pharmacol. Volume: 171 Issue: 1 Pages: 202-213
  • DOI: 10.1111/bph.12471
  • Peer Reviewed
• [Journal Article] Central bombesin possibly induces S-nitrosylation of cyclooxygenase-1 in pre-sympathetic neurons of rat hypothalamic paraventricular nucleus (2014)
  • Author(s): Tanaka K, Shimizu T, Higashi Y, Nakamura K, Taniuchi K, Dimitriadis F, Shimizu S, Yokotani K, Saito M.
  • Journal Title: Life Sci. Volume: 100 Issue: 2 Pages: 85-96
  • DOI: 10.1016/j.lfs.2014.01.079
  • Peer Reviewed
• [Journal Article] Possible involvement of brain prostaglandin E2 and prostanoid EP3 receptors in prostaglandin E2 glycerol ester-induced activation of central sympathetic outflow in the rat (2014)
  • Author(s): Shimizu T, Tanaka K, Nakamura K, Taniuchi K, Yawata T, Higashi Y, Ueba T, Dimitriadis F, Shimizu S, Yokotani K, Saito M.
  • Journal Title: Neuropharmacology Volume: 82 Pages: 19-27
  • DOI: 10.1016/j.neuropharm.2014.03.005
  • Peer Reviewed / Open Access
• [Journal Article] RUVBL1 directly binds actin filaments and induces formation of cell protrusions to promote pancreatic cancer cell invasion. (2014)
  • Author(s): Taniuchi K, Furihata M, Iwasaki S, Tanaka K, Shimizu T, Saito M, Saibara T
  • Journal Title: International Journal of Oncology Volume: 44 Issue: 6 Pages: 1945-1954
  • DOI: 10.3892/ijo.2014.2380
  • Peer Reviewed
• [Journal Article] BART inhibits pancreatic cancer cell invasion by Rac1 inactivation through direct binding to active Rac1. (2012)
  • Author(s): Taniuchi K, Yokotani K, Saibara T
  • Journal Title: Neoplasia Volume: 14 Issue: 1-3 Pages: 440-50
  • DOI: 10.1016/j.ejphar.2012.08.002
  • Peer Reviewed
• [Journal Article] BART inhibits pancreatic cancer cell invasion by PKC alpha inactivation through binding to ANX7 (2012)
  • Author(s): Taniuchi K, Saibara T
  • Journal Title: PLoS One Volume: 7 Issue: 4 Pages: e35674-e35674
  • DOI: 10.1371/journal.pone.0035674
  • Peer Reviewed
• [Journal Article] Brain phospholipase C, diacylglycerol lipase and monoacylglycerol lipase are involved in (±)-epibatidine-induced activation of central adrenomedullary outflow in rats. (2012)
  • Author(s): Takahiro Shimizu
  • Journal Title: European Journal of Pharmacology Volume: 691 Issue: 1-3 Pages: 93-102
  • DOI: 10.1016/j.ejphar.2012.07.017
  • Peer Reviewed
• [Journal Article] Meckel's diverticulum preoperatively diagnosed by double-balloon endoscopy. (2012)
  • Author(s): Taniuchi K, Tanaka H, Iwamura S, Mori I.
  • Journal Title: Intern Med Volume: 51 Pages: 1023-6
  • NAID: 130002061792
  • Peer Reviewed
• [Presentation] 前立腺肥大症に対するα1受容体遮断薬シロドシンの前立腺血流を介した抑制効果 (2014)
  • Author(s): 清水翔吾、清水孝洋、谷内恵介、東洋一郎、中村久美子、Holmstrom Felix、 齊藤源顕
  • Organizer: 第67回日本薬理学会西南部会
  • Place of Presentation: 北九州（産業医科大学ラマツィーニホール）
  • Year and Date: 2014-11-23
• [Presentation] ATP感受性カリウムチャネル阻害による精巣虚血再灌流障害の軽減効果 (2014)
  • Author(s): 清水翔吾、清水孝洋、谷内恵介、齊藤源顕
  • Organizer: 第66回日本生理学会中国四国地方会
  • Place of Presentation: 高松（情報通信交流館eとぴあ・かがわ）
  • Year and Date: 2014-11-01 – 2014-11-02
• [Presentation] 細胞内ストレス顆粒に局在するCD24の膵癌転移抑制機序の解析 (2014)
  • Author(s): 谷内恵介, 降幡睦夫
  • Organizer: 第73回日本癌学会総会
  • Place of Presentation: 横浜（パシフィコ横浜）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] RUVBL1はアクチンとの結合を介して膵癌細胞の運動・浸潤を亢進させる (2014)
  • Author(s): 坪井麻記子, 谷内恵介, 降幡睦夫
  • Organizer: 第73回日本癌学会総会
  • Place of Presentation: 横浜（パシフィコ横浜）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Prdx1はp38 MAPKの活性制御を介して膵癌細胞の運動・浸潤を亢進させる (2014)
  • Author(s): 田内亜紀, 谷内恵介, 降幡睦夫
  • Organizer: 第73回日本癌学会総会
  • Place of Presentation: 横浜（パシフィコ横浜）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] α1 受容体遮断薬シロドシンによる前立腺血流量改善を介した前立腺肥大の抑制効果 (2014)
  • Author(s): 清水 翔吾、Tsounapi Panagiota、清水 孝洋、谷内 恵介、本田 正史、井上 啓史、武中 篤、齊藤 源顕
  • Organizer: 第21回日本排尿機能学会
  • Place of Presentation: 岡山(岡山コンベンションセンター)
  • Year and Date: 2014-09-17 – 2014-09-20
• [Presentation] Inhibitory involvement of brain cannabinoid CB1 receptors in central activation of sympatho-adrenomedullary outflow induced by bombesin microinjected into the rat hypothalamic paraventricular nucleus (2014)
  • Author(s): Shimizu T, Tanaka K, Nakamura K, Taniuchi K, Shimizu S, Yawata T, Higashi Y, Ueba T, Yokotani K, Saito M
  • Organizer: 17th World congress of basic & clinical pharmacology
  • Place of Presentation: Cape Town, South Africa
  • Year and Date: 2014-07-13 – 2014-07-18
• [Presentation] The effect of the ATP sensitive potassium channel blocker, glibenclamide in the ischemia-reperfusion injury in the rat testis. (2014)
  • Author(s): Shimizu S, Tsounapi P, Shimizu T, Tanaka K, Taniuch K, Honda M, Inoue K, Takenaka A, Tomita S, Saito M
  • Organizer: 第33回日本アンドロロジー学会学術大会
  • Place of Presentation: 軽井沢（軽井沢プリンスホテルウエスト）
  • Year and Date: 2014-06-12 – 2014-06-13
• [Presentation] Prdx1 promotes pancreatic cancer cell motility and invasion by modulating p38 MAPK activity (2014)
  • Author(s): Taniuchi K, Furihata M, Iwasaki S, Shimizu S, Shimizu T, Saito M, Saibara T
  • Organizer: United European Gastroenterology Week
  • Place of Presentation: Vienna, Austria
• [Presentation] RUVBL1 directly binds actin filaments and induces formation of cell protrusions to promote pancreatic cancer cell invasion (2014)
  • Author(s): Taniuchi K, Furihata M, Iwasaki S, Shimizu S, Shimizu T, Saito M, Saibara T
  • Organizer: Asian Pacific Digestive Week
  • Place of Presentation: Bali, Indonesia
• [Presentation] KIF20AはRNA結合蛋白質とメッセンジャーRNAの複合体を輸送することにより膵癌浸潤・転移を亢進させる (2014)
  • Author(s): 谷内恵介, 岩﨑信二, 西原利治
  • Organizer: 第100回日本消化器病学会総会
  • Place of Presentation: 東京
• [Presentation] KIF20A結合型RNA結合蛋白質を標的とした膵癌新規治療法の確立に向けて (2014)
  • Author(s): 谷内恵介
  • Organizer: 第45回日本膵臓学会大会
  • Place of Presentation: 北九州
• [Presentation] B0031 enhances pancreatic cancer cell motility and invasion through binding to ANXA2. (2013)
  • Author(s): Taniuchi K, Furihata M, Saito M, Saibara T
  • Organizer: Joint Meeting of the International Association of Pancreatology & the Korean Pancreatobiliary Association
  • Place of Presentation: Seoul, Korea
• [Presentation] ヘモグロビンRVD-ヘモプレッシンは脳内カンナビノイドCB1受容体を介してボンベシンによる中枢性副腎髄質系賦活を抑制する (2013)
  • Author(s): 清水孝洋, 田中健二朗, 柳田俊彦, 根本隆行, 中村久美子, 谷内恵介, 横谷邦彦, 齊藤源顕
  • Organizer: 第66回日本自律神経学会総会
  • Place of Presentation: 名古屋
• [Presentation] KIF20AがRNA結合蛋白質を介して膵癌浸潤・転移を亢進させる機序 (2013)
  • Author(s): 谷内恵介
  • Organizer: 第44回日本膵臓学会大会
  • Place of Presentation: 仙台
• [Presentation] BART inhibits pancreatic cancer cell motility and invasion through Rac1 inactivation through direct binding to active forms of Rac1 (2013)
  • Author(s): Taniuchi K, Yokotani K, Saibara T
  • Organizer: Ninth AACR-Japanese Cancer Association Joint Conference, 2013
  • Place of Presentation: ハイアットリージェンシー、マウイ （米国）
• [Presentation] B0031による膵癌細胞の浸潤亢進機序の解析 (2012)
  • Author(s): 田内亜紀, 降幡睦夫, 谷内恵介
  • Organizer: 第71回日本癌学会総会
  • Place of Presentation: ホテルロイトン札幌、さっぽろ芸文館、札幌市教育文化会館 （北海道）
• [Presentation] BARTはRac1活性を制御することにより膵癌細胞の形態を変化させ、膵癌細胞の遊走・浸潤を制御する (2012)
  • Author(s): 谷内恵介, 横谷邦彦, 西原利治
  • Organizer: 第44回日本臨床分子形態学会総会
  • Place of Presentation: かるぽーと （高知）