Functional analysis and regulation of microvesicles that affect tumor-stromal interaction in pancreatic cancer.

• Project/Area Number: 24591014
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Kyushu University
• Principal Investigator: SHIMIZU Yukiko 九州大学, 大学病院, 助教 (10404021)
• Co-Investigator(Kenkyū-buntansha): MIZUMOTO Kazuhiro 九州大学, 大学病院, 准教授 (90253418) ; TOMINAGA Yohei 九州大学, 医学研究院, 共同研究員 (90304823) ; EGAMI Takuya 九州大学, 医学研究院, 共同研究員 (40507787) ; MIYASAKA Yoshihiro 九州大学, 医学研究院, 助教 (40507795) ; OHUCHIDA Kenoki 九州大学, 大学病院, 助教 (20452708)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 膵癌 / 膵星細胞 / ミクロベシクル / 癌間質相互作用 / マイクロベシクル / ギャップジャンクション

Outline of Final Research Achievements

The aim of this study is to identify and analyse the microvesicles affecting tumor-stromal interaction in pancreatic cancer. To clarify the functional interaction between pancreatic cancer cells and pancreatic stellate cells (PSCs), we focused CD146 expression in PSCs. Immunohistochemistry revealed that CD146 positive PSCs were around the PanIN, the premalignant lesion of pancreatic cancer, but around the invasive carcinoma there was few CD146 positive PSCs. This result indicating that CD146 positive PSCs have suppressive function for pancreatic cancer cells, and some kind of soluble factor or microvesicles are mediating between them.

Research Products

• [Journal Article] Peritoneal myofibroblasts at metastatic foci promote dissemination of pancreatic cancer. (2014)
  • Author(s): Akagawa S, Ohuchida K, Torata N, Hattori M, Eguchi D, Fujiwara K, Kozono S, Cui L, Ikenaga N, Ohtsuka T, Oishima S, Mizumoto K, Oda Y, Tanaka M
  • Journal Title: Int J Oncol Volume: 45 Issue: 1 Pages: 113-120
  • DOI: 10.3892/ijo.2014.2391
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Micro RNA-373 is Down-regulated in Pancreatic Cancer and Inhibits Cancer Cell Invasion (2014)
  • Author(s): Nakata K, Ohuchida K, Mizumoto K, Aishima S, Oda Y, Nagai E, Tanaka M
  • Journal Title: Ann Surg Oncol Volume: 21 Issue: S4 Pages: 564-574
  • DOI: 10.1245/s10434-014-3676-8
  • Peer Reviewed
• [Journal Article] CD166/ALCAM expression is characteristic of tumorigenicity and invasive and migratory activities of pancreatic cancer cells. (2014)
  • Author(s): Fujiwara K, Ohuchida K, Sada M, Horioka K, Ulrich CD 3rd, Shindo K, Ohtsuka T, Takahata S, Mizumoto K, Oda Y, Tanaka M.
  • Journal Title: PLoS One Volume: 9 Issue: 9 Pages: 1-11
  • DOI: 10.1371/journal.pone.0107247
  • Peer Reviewed / Open Access
• [Journal Article] CD271^+ subpopulation of pancreatic stellate cells correlates with prognosis of pancreatic cancer and is regulated by interaction with cancer cells (2012)
  • Author(s): Kenji Fujiwara
  • Journal Title: PLoS One Volume: 12 Issue: 12 Pages: e52682-e52682
  • DOI: 10.1371/journal.pone.0052682
  • Peer Reviewed
• [Presentation] 膵癌関連繊維芽細胞におけるCD146発現と膵がんの進展抑制 (2014)
  • Author(s): 鄭彪、大内田研宙、崔林、水内祐介、藤原謙次、赤川進、堀岡宏平、田中友晴、佐田政史、仲田興平、前山良、大塚隆生、小田義直、水元一博、田中雅夫
  • Organizer: 第45回日本膵臓学会大会
  • Place of Presentation: 北九州市
  • Year and Date: 2014-07-11
• [Presentation] 膵癌におけるCD271陽性膵星細胞の意義
  • Author(s): 藤原 謙次 他
  • Organizer: 第112回日本外科学会定期学術集会
  • Place of Presentation: 千葉
• [Presentation] CD105陽性膵癌細胞は膵星細胞から影響を受けて強い遊走能を獲得する
  • Author(s): 藤原 謙次 他
  • Organizer: 第43回日本膵臓学会大会
  • Place of Presentation: 山形
• [Presentation] CD271+ pancreatic stellate cells are correlated with prognosis of patients with pancreatic cancer and regulated by interaction with cancer cells
  • Author(s): Kenji Fujiwara et al.
  • Organizer: International Symposium on Pancreas Cancer 2012 in Kyoto
  • Place of Presentation: 京都
• [Presentation] Migratory activity of CD105+ pancreatic cancer cells is strongly enhanced by pancreatic stellate cells
  • Author(s): Kenji Fujiwara et al.
  • Organizer: 2012 Joint Meeting of APA and IAP
  • Place of Presentation: Miami, USA
• [Presentation] A CD166 negative subpopulation of pancreatic cancer cells has strong invasive and migratory activity.
  • Author(s): Kenji Fujiwara et al.
  • Organizer: 4th Biennial Congress of the Asian-Pacific Hepato-Pancreato-Billiary Association
  • Place of Presentation: 上海、中国
• [Presentation] Fibroblasts expressing Podoplanin enhance the tumor progression
  • Author(s): Koji Shindo et al.
  • Organizer: 2012 Joint Meeting of APA and IAP
  • Place of Presentation: Miami, USA
• [Presentation] Fibroblasts expressing Podoplanin enhance the tumor progression of invasive ductal carcinoma of pancreas
  • Author(s): 進藤 幸治 他
  • Organizer: 第71回日本癌学会
  • Place of Presentation: 札幌