Development of nanocapsule containing MRI contrast agent effective for early diagnosis of pancreatic cancer

• Project/Area Number: 24591015
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Kyushu University
• Principal Investigator: SAKAI Hiroshi 九州大学, 医学(系)研究科(研究院), 共同研究員 (80611665)
• Co-Investigator(Kenkyū-buntansha): SHIRAHANE Kengo 九州大学, 医学研究院, 共同研究員 (10529803) ; TOMINAGA Yohei 九州大学, 医学研究院, 共同研究員 (90304823) ; MORIYAMA Taiki 九州大学, 大学病院, 助教 (70586859) ; OHUCHIDA Kenoki 九州大学, 大学病院, 助教 (20452708)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 膵癌 / 早期診断 / ナノカプセル / MRI

Outline of Final Research Achievements

In order to create a new image analysis for early detection of pancreatic cancer, we planned to make a nanocapsule containing contrast medium for MRI which could specifically accumulate for pancreatic cancer cells. First, we aimed to identify specific molecules for pancreatic cancer cells and analyzed about surface proteins, CD74 and CD110. In human tissue samples, immunohistochemistry analysis revealed that expression of CD74 and CD110 in pancreatic cancer cells was stronger than in normal pancreatic ductal cells. Knock down of CD74 decreased proliferation, migration, and invasion of pancreatic cancer cells. In addition, knock down of CD110 decreased migration and invasion, and decreased proliferation of pancreatic cancer cells in the presence of thrombopoietin. CD74 and CD110 could be a diagnostic marker of pancreatic cancer.

Research Products

• [Journal Article] CD166/ALCAM expression is characteristic of tumorigenicity and invasive and migratory activities of pancreatic cancer cells. (2014)
  • Author(s): Fujiwara K, Ohuchida K, Sada M, Horioka K, Ulrich CD 3rd, Shindo K, Ohtsuka T, Takahata S, Mizumoto K, Oda Y, Tanaka M.
  • Journal Title: PLoS One Volume: 9 Issue: 9 Pages: 1-11
  • DOI: 10.1371/journal.pone.0107247
  • Peer Reviewed / Open Access
• [Journal Article] Peritoneal myofibroblasts at metastatic foci promote dissemination of pancreatic cancer. (2014)
  • Author(s): Akagawa S, Ohuchida K, Torata N, Hattori M, Eguchi D, Fujiwara K, Kozono S, Cui L, Ikenaga N, Ohtsuka T, Oishima S, Mizumoto K, Oda Y, Tanaka M
  • Journal Title: Int J Oncol Volume: 45 Issue: 1 Pages: 113-120
  • DOI: 10.3892/ijo.2014.2391
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Micro RNA-373 is Down-regulated in Pancreatic Cancer and Inhibits Cancer Cell Invasion (2014)
  • Author(s): Nakata K, Ohuchida K, Mizumoto K, Aishima S, Oda Y, Nagai E, Tanaka M
  • Journal Title: Ann Surg Oncol Volume: 21
  • Peer Reviewed
• [Presentation] 膵癌の肝転移におけるfollistatin-like1の役割 (2014)
  • Author(s): 堀岡宏平、大内田研宙、江口大樹、佐田政史、田中友晴、赤川進、藤原謙次、鄭彪、仲田興平、宮坂義浩、前山良、大塚隆生、水元一博、田中雅夫
  • Organizer: 第114回日本外科学会定期学術集会
  • Place of Presentation: 京都市
  • Year and Date: 2014-04-04
• [Presentation] 膵癌におけるTM4SF1発現の意義 (2014)
  • Author(s): 鄭彪、崔林、大内田研宙、進藤幸治、藤原謙次、赤川進、堀岡宏平、田中友晴、佐田政史、吉田真樹、千々岩芳朗、奥村隆志、仲田興平、大塚隆生、小田義直、水元一博、田中雅夫
  • Organizer: 第114回日本外科学会定期学術集会
  • Place of Presentation: 京都市
  • Year and Date: 2014-04-04
• [Presentation] Role of Peritoneal Fibroblasts at Disseminated Sites of Pancreatic Cancer
  • Author(s): Shin Akagawa et al
  • Organizer: 2012 Joint Meeting of APA and IAP
  • Place of Presentation: Miami, USA
• [Presentation] 腹膜線維芽細胞が膵癌の腹膜播種を促進する
  • Author(s): 赤川 進 他
  • Organizer: 第43回日本膵臓学会
  • Place of Presentation: 山形