Investigation of primary culture system for pancreatic cancer stem cells

• Project/Area Number: 24591017
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Keio University
• Principal Investigator: HAJIME Higuchi 慶應義塾大学, 医学部, 講師 (20306682)
• Co-Investigator(Kenkyū-buntansha): SATO Toshiro 慶應義塾大学, 医学部, 特任准教授 (70365245)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 癌幹細胞 / 上皮間葉転換 / CD44 / 膵癌 / 間葉系幹細胞 / 膵癌幹細胞 / 3次元培養 / 胆道学 / 膵臓学 / 三次元培養

Outline of Final Research Achievements

Primary culturing or long-term culturing of pancreatic cancer tissue is important technology for the study of cancer biology and investigation of molecular specific treatment strategy. We performed long-term culturing of primary pancreatic cancer tissue using matrigel 3D culture system. The pancreatic cancer cells were enriched with CD44-sprice variant-positive cells, so-called cancer stem cell-like population. In addition, we found that co-culturing of pancreatic cancer cells with mesenchymal stem cells (MSCs) enhanced cancer stem cell-like properties as well as epithelial-to-mesenchymal transition within the cancer cells. The matrigel 3D culture system or co-culturing with MSCs is an attractive technology for in vitro analysis of cancer biology.

Research Products

• [Journal Article] Mesenchymal Stem Cells Regulate Epithelial-to-Mesenchymal Transition and Tumor Progression of Pancreatic Cancer Cells. (2013)
  • Author(s): Kabashima-Niibe A, Higuchi H, Takaishi H, Masugi Y, Matsuzaki Y, Mabuchi Y, Funakoshi S, Adachi M, Hamamoto Y, Kawachi S, Aiura K, Kitagawa Y, Sakamono M, Hibi T.
  • Journal Title: Cancer Sci. Volume: 104(2) Pages: 157-164
  • Peer Reviewed
• [Presentation] Mesenchymal stem cells mediate epithelial-to-mesenchymal transition (EMT) (2013)
  • Author(s): A. Kabashima-Niibe, H. Higuchi, Y. Matsuzaki, et. al.
  • Organizer: American Association for Cancer Research 104th Annual Meeting,
  • Place of Presentation: Washington D.C