Application of inhibitory method of pancreatic cancer cell growth by the regulation of lumican glycosylation using therapeutic peptides.
Project/Area Number |
24591019
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Gastroenterology
|
Research Institution | Kinki University (2013-2014) Nippon Medical School (2012) |
Principal Investigator |
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | 膵臓癌 / ルミカン / プロテオグリカン / プロテオミクス / アポトーシス / lumican / 糖鎖修飾 / プロテオーム解析 |
Outline of Final Research Achievements |
It has been reported that lumican expression level showed positive correlation with cell growth of pancreatic ductal adenocarcinoma (PDAC) cells and showed negative correlation with cell invasion of PDAC cells. In this study, we performed global shotgun proteomic analysis using lumican-regulated PANC-1 cells to examine the effect of lumican on cell growth and invasion in PDAC cells. As a result of proteomics, 24 proteins were identified as candidate proteins which expression was regulated by lumican, and these candidate proteins included protein which is known apoptosis marker and protein which regulate MMP-9 activation. Thus, lumican might be involved in cell growth and invasion to regulate cell apoptosis and these proteins expression.
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Report
(4 results)
Research Products
(5 results)