| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Outline of Final Research Achievements |
Background: Fluorescence polarization (FP) is an optical method to measure cellular membrane fluidity. We showed that high reactive oxygen species involved cellular lipid peroxidation to increase its membrane fluidity in cancer cells. We thus investigated that the relation among tumor differentiation, lipid peroxidation and membrane fluidity in colon cancer.. METHODS: Fourteen specimens of colon cancer were investigated. FP values of each sample were measured with a specially designed system. We compared each FP value of the specimens with the result of pathological examinations to evaluate the relation membrane fluidity and tumor differentiation. RESULTS: FP value of normal area was significantly higher than that of adenoma and adenocarcinoma area. Amount of lipid peroxidation estimated by 8OH-dG stain, membrane fluidity were significantly increased in than those of cancerous area than those of normal area. CONCLUSION: The FP analysis was demonstrated to be clue to an optical biopsy.
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