Investigation of endoscopic and molecular diagnosis of colorectal serrated polyps

• Project/Area Number: 24591029
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Showa University
• Principal Investigator: KONISHI KAZUO 昭和大学, 医学部, 講師 (60307014)
• Co-Investigator(Kenkyū-buntansha): 久保田 祐太郎 昭和大学, 医学部, 講師 (80465103)
• Research Collaborator: KOBA Ikuro ; Ohata Ken ; MATSUYAMA Kyoji ; YAMOCHI Toshiko ; FUTAMURA Satoshi
• Project Period (FY): 2012-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 大腸鋸歯状腺腫 / 内視鏡診断 / 大腸癌 / 大腸発癌 / 大腸内視鏡検査 / 大腸ポリープ / 大腸鋸歯状ポリープ / 鋸歯状腺腫 / 内視鏡 / DNAメチル化 / 大腸内視鏡

Outline of Final Research Achievements

We performed the prospective study of differential endoscopic features of sessile serrated adenomas in the colorectum and concluded that the presence of mucus on the lesions may be a practicable finding for the differential diagnosis of sessile serrated adenoma/polyps (SSA/Ps) and hyperplastic polyps. Our molecular analysis suggested that distinct molecular features were observed between proximal and distal serrated polyps with hyperplastic crypt pattern and that　ｐroximal microvesicular hyperplastic polyps (MVHPs) may develop more frequently through SSA/Ps to CpG island methylator phenotype (CIMP) cancers than dist al MVHPs. We performed methylated CpG island amplification microarrays in three paired proximal MVHPs and SSA/Ps with/without cytological dysplasia and found new candidate genes associated with the serrated pathway.

Research Products

• [Journal Article] Differential diagnosis of colorectal serrated polyps using conventional endoscope (2017)
  • Author(s): Yanagisawa F, Kubota Y, Konishi K, Katagiri K, Muramoto T, Kihara T, Yano Y, Tojo M, Shinmura K, Konda K, Tagawa T, Iijima K, Gocho T, Yamamura F, Yamochi T, Takimoto M, Yoshida H
  • Journal Title: Showa Univ J Med Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Magnifying colonoscopic findings for differential diagnosis of sessile serrated adenoma/polyps and hyperplastic polyps. (2016)
  • Author(s): Kihara T, Kubota Y, Konishi K, Katagiri A, Muramoto T, Yano Y, Tojo M, Shinmura K, Konda K, Tagawa T, Yanagisawa F, Iijima K, Gocho T, Yamamura F, Yamochi T, Takimoto M, Yoshida H.
  • Journal Title: Showa Univ J Med Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Molecular features of colorectal polyps presenting Kudo’s type II mucosal crypt pattern: are they based on the same mechanism of tumorigenesis? (2014)
  • Author(s): Shinmura K, Konishi K, Yamochi T, et al.
  • Journal Title: Endoscopy International Open Volume: 02 Issue: 03 Pages: E171-E177
  • DOI: 10.1055/s-0034-1377518
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Distinct molecular features of different macroscopic subtypes of colorectal neoplasms (2014)
  • Author(s): Konda K, Konishi K, Yamochi T, et al.
  • Journal Title: PLoS ONE Volume: 9 Issue: 8 Pages: e103822-e103822
  • DOI: 10.1371/journal.pone.0103822
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Clinicopathological and molecular features in laterally spreading tumors (2014)
  • Author(s): Konda K, Konishi K, Katagiri A, et al.
  • Journal Title: Showa Univ J Med Volume: 26 Pages: 39-45
  • NAID: 130004691843
  • Peer Reviewed
• [Journal Article] 治療に直結する大腸腫瘍診断のストラテジー，大腸LSTに対するpit診断のpitfall (2013)
  • Author(s): 小西一男，片桐 敦
  • Journal Title: 消化器内視鏡 Volume: 25 Pages: 1262-1263
  • Peer Reviewed
• [Journal Article] 【SSA/Pの本態を探る】遺伝子研究の立場から SSA/Pと過形成性ポリープにおける分子生物学的特徴. (2012)
  • Author(s): 小西一男, 久保田祐太郎, 矢野雄一郎, 片桐敦, 村元喬, 小林祥也, 東條正幸, 紺田健一, 新村健介, 矢持淑子.
  • Journal Title: Intestine Volume: 16 Pages: 541-548
  • Peer Reviewed
• [Journal Article] 【転移をきたす小さな大腸癌-本当に悪性の大腸癌とは?】「小さな大腸癌」の特徴(遺伝子の立場から) (2012)
  • Author(s): 小西一男, 片桐敦, 久保田祐太郎, 村元喬, 矢野雄一郎, 小林祥也, 東條正幸, 紺田健一, 新村健介, 矢持淑子, 井廻道夫.
  • Journal Title: Intestine Volume: 16 Pages: 378-386
  • Peer Reviewed
• [Presentation] Distinct clinicopathological and molecular features between laterally spreading tumors and polypoid neoplasms in the colorectum (2015)
  • Author(s): Kihara T, Kubota Y, Konishi K, Nozawa H, Yano Y, Tojo M, Shinmura K, Konda K, Tagawa T, Yanagisawa F, Iijima K, Gocho T, Makino R, Takimoto M, Yoshida H
  • Organizer: 23th United European Gastroenterology Week
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2015-10-25
  • Int'l Joint Research
• [Presentation] 大腸側方発育型腫瘍の臨床病理学的および分子生物学的特徴に関する検討 (2015)
  • Author(s): 木原俊裕，久保田祐太郎，小西一男，牛腸俊彦，柳澤文人，飯島堅太郎，田川徹平，紺田健一，新村健介，東條正幸，矢野雄一郎，竹内義明，山村冬彦，吉田 仁
  • Organizer: 第23回消化器関連学会週間
  • Place of Presentation: 東京
  • Year and Date: 2015-10-08
• [Presentation] Distinct molecular features of different macroscopic subtypes of colorectal neoplasms (2014)
  • Author(s): Konda K, Konishi K, Yamochi T, Ito YM, Nozawa H, Tojo M, Shinmura K, Kogo M, Katagiri A, Kubota Y, Muramoto T, Yano Y, Kihara T, Tagawa T, Yanagisawa F, Iijima K, Makino R, Takimoto M, Yoshida H
  • Organizer: 22th United European Gastroenterology Week
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2014-10-18 – 2014-10-22
• [Presentation] Distinct clinicopathological and molecular features of laterally spreading tumors in the colorectum (2014)
  • Author(s): Kihara T, Kubota Y, Konishi K, Nozawa H, Katagiri A, Yano Y, Tojo M, Shinmura K, Konda K, Tagawa T, Yanagisawa F, Iijima K, Yoshida H.
  • Organizer: 22th United European Gastroenterology Week
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2014-10-18 – 2014-10-22
• [Presentation] 大腸鋸歯状病変における大腸拡大内視鏡観察の有用性 (2014)
  • Author(s): 木原俊裕，小西一男，久保田祐太郎，片桐 敦，村元 喬，矢野雄一郎，東條正幸，紺田健一，新村健介，田川徹平，柳澤文人，飯島堅太郎，山村冬彦，吉田 仁．
  • Organizer: 第98回日本消化器内視鏡学会関東地方会
  • Place of Presentation: 東京
  • Year and Date: 2014-06-14 – 2014-06-15
• [Presentation] Molecular features of colorectal polyps presenting Kudo’s type II pit pattern (2014)
  • Author(s): Shinmura K, Konishi K, Kubota Y, Yano Y, Katagiri A, Muramoto T, Kobayashi Y, Kihara T, Tojo M, Konda K, Tagawa T , Yamochi T, Yoshida H.
  • Organizer: Digestive Disease Week2014
  • Place of Presentation: Chicago, IL
  • Year and Date: 2014-05-03 – 2014-05-06
• [Presentation] Molecular features of colorectal polyps presenting Kudo’s type II pit pattern. (2013)
  • Author(s): Shinmura K, Konishi K, Kubota Y, Yano Y, Katagiri A, Muramoto T, Kobayashi Y, Kihara T, Tojo M, Konda K, Tagawa T , Yamochi T, Yoshida H.
  • Organizer: 21th United European Gastroenterology Week
  • Place of Presentation: Berlin, Germany
• [Presentation] Endoscopic pit pattern for differential diagnosis of colorectal serrated polyps: Can it distinguish sessile serrated adenoma from hyperplastic polyp? (2013)
  • Author(s): Kihara T, Kubota Y, Konishi K, Yano Y, Katagiri A, Muramoto T, Tojo M, Konda K, Shinmura K, Tagawa T, Yamochi T, Yoshida H.
  • Organizer: 21th United European Gastroenterology Week
  • Place of Presentation: Berlin, Germany
• [Presentation] Molecular features of colorectal polyps presenting Kudo’s type II mucosal crypt pattern. (2013)
  • Author(s): Shinmura K, Konishi K, Kubota Y, Yano Y, Nozawa H, Katagiri A, Muramoto T, Kobayashi Y, Kihara T, Tojo M, Konda K, Tagawa T , Yamochi T, Takimoto M, Yoshida H.
  • Organizer: Digestive Disease Week2013
  • Place of Presentation: Orland, FL
• [Presentation] Endoscopic features for differential diagnosis of colorectal serrated polyps: A prospective study. (2013)
  • Author(s): Tojo M, Konishi K, Kubota Y, Yano Y, Katagiri A, Muramoto T, Kobayashi Y, Kihara T, Konda K, Shinmura K, Tagawa T , Yamochi T, Takimoto M, Yoshida H.
  • Organizer: Digestive Disease Week2013
  • Place of Presentation: Orland, FL
• [Presentation] Five different subclasses of colorectal neoplasias identified by integrated genetic and epigenetic analysis: Association with polypoid and nonpolypoid carcinogenesis. (2013)
  • Author(s): Konda K, Konishi K, Katagiri A, Tojo M, Kubota Y, Muramoto T, Yano Y, Kobayashi Y, Kihara T, Shinmura K, Tagawa T , Yamochi T, Ito Y, Imawari M, Yoshida H.
  • Organizer: Digestive Disease Week2013
  • Place of Presentation: Orland, FL
• [Presentation] 内視鏡的II型腺口形態を示す大腸鋸歯状病変における臨床病理学的・分子生物学的所見に関する検討． (2013)
  • Author(s): 新村健介，小西一男，矢野雄一郎，片桐 敦，久保田祐太郎，村元 喬，木原俊裕，小林祥也，東條正幸，紺田健一，田川徹平，矢持淑子，吉田 仁．
  • Organizer: 第86回日本消化器内視鏡学会総会
  • Place of Presentation: 東京
• [Presentation] 大腸上皮性腫瘍におけるエピジェノタイプと臨床病理学的特徴． (2013)
  • Author(s): 紺田健一，小西一男，吉田 仁．
  • Organizer: 第85回日本消化器内視鏡学会総会
  • Place of Presentation: 京都
• [Presentation] 主題I ”右側結腸の謎に迫る” 基調講演「大腸癌の占居部位別からみた臨床病理学的・生物学的特徴」． (2013)
  • Author(s): 小西一男
  • Organizer: 第9回鬼怒川フォーラム
  • Place of Presentation: 焼津，静岡
  • Invited
• [Presentation] GENETIC AND EPIGENETIC FEATURES THAT DISTINGUISH POLYPOID FROM NONPOLYPOID COLORECTAL NEOPLASIAS (2012)
  • Author(s): Kenichi Konda, Kazuo Konishi, Toshiko Yamochi, Yuichiro Yano, et al.
  • Organizer: The 20th United European Gastroenterology Week 2012
  • Place of Presentation: Amsterdam, Netherlands
• [Presentation] Endoscopic features of colorectal serrated polyps. (2012)
  • Author(s): Yutaro Kubota, Kazuo Konishi, Toshiko Yamochi, Yuichiro Yano, et al.
  • Organizer: The 20th United European Gastroenterology Week 2012
  • Place of Presentation: Amsterdam, Netherlands
• [Presentation] THE ENDOSCOPIC MUCOSAL CRYPT PATTERNS PREDICT THE UNIQUE CLINICOPATHOLOGICAL AND MOLECULAR FEATURES OF SERRATED ADENOMAS. (2012)
  • Author(s): Masayuki Tojo, Kazuo Konishi, Yuichiro Yano, Yutaro Kubota, et al.
  • Organizer: The 20th United European Gastroenterology Week 2012
  • Place of Presentation: Amsterdam, Netherlands
• [Presentation] 大腸鋸歯状病変の内視鏡的腺口形態と臨床病理学的・分子生物学的所見についての検討. (2012)
  • Author(s): 矢野雄一郎、小西一男, 片桐敦, 久保田祐太郎, 村元喬, 小林祥也, 東條正幸, 紺田健一, 新村健介, 吉川望海, 井廻道夫.
  • Organizer: 第83回日本消化器内視鏡学会総会
  • Place of Presentation: 東京
• [Presentation] 大腸鋸歯状病変における内視鏡的腺口形態と臨床病理学的・分子生物学的特徴. (2012)
  • Author(s): 久保田祐太郎, 小西一男, 片桐敦, 村元喬, 矢野雄一郎, 小林祥也, 東條正幸, 紺田健一, 新村健介, 吉川望海, 井廻道夫
  • Organizer: 第84回日本消化器内視鏡学会
  • Place of Presentation: 神戸，兵庫
• [Presentation] 内視鏡的II型腺口形態を示す大腸鋸歯状病変における臨床病理学的・分子生物学的所見に関する検討．
  • Author(s): 新村健介，小西一男，吉田 仁．
  • Organizer: 第60回昭和大学学士会総会
  • Place of Presentation: 東京
• [Presentation] Sessile serrated adenoma/polypと過形成性ポリープの内視鏡的鑑別診断能に関する検討.
  • Author(s): 久保田祐太郎, 小西一男, 矢野雄一郎, 片桐敦, 村元喬, 小林祥也, 東條正幸, 紺田健一, 新村健介, 田川徹平
  • Organizer: 第9回拡大内視鏡研究会
  • Place of Presentation: 東京
• [Book] 内視鏡医のための大腸ポリープマネジメント (2015)
  • Author(s): 小西一男，木原俊裕，久保田祐太郎
  • Total Pages: 6
  • Publisher: 日本メディカルセンター