| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Heart failure is one of the leading causes of death in Japan and the novel diagnosis and treatment are strongly awaited. To overcome this issue, we aimed to elucidate the molecular mechanisms of cardiac induction in embryonic stem cells (ES cells). Comprehensive gene expression analysis using ES cells identified Asb2, which is thought to be an E3 ligase. Further analysis revealed that Asb2 is specifically expressed in the heart and skeletal muscle during development. In silico data base suggested potential interaction between Asb2 and Smad9, and we confirmed that Asb2 is the E3 ligase for Smad9 and leads it to proteasomal degradation. Our data suggested that Asb2-dependent Smad9 degradation plays important role in the development of the heart through quantitative regulation of Smad9 and thus the regulation of BMP signaling.
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