| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The role of epigenetics in aldosterone-producing adenomas (APA) is not fully understood. Here we explored the relationship between gene expression and DNA methylation status in APA. We conducted an integrated analysis of transcriptome and methylome data of the paired APA-adjacent adrenal gland (AAG) samples from the same patient. The adrenal specimens were obtained from 7 Japanese patients with APA. Gene expression and genome-wide CpG methylation profiles were obtained from RNA and DNA samples from those 7 paired tissues. Methylome analysis showed global CpG hypomethylaiton in APA relative to AAG. Integration of gene expression and methylation status showed that 34 genes are upregulated with CpG hypomethylation in APA. Of these, 3 genes (CYP11B2, MC2R and HPX) may be related to aldosterone production and 5 genes (PRRX1, RAB38, FAP, GCNT2 and ASB4) are potentially involved in tumorigenesis.
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