| Project/Area Number |
24591128
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Mie University |
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Principal Investigator |
GABAZZA Esteban 三重大学, 医学(系)研究科(研究院), 教授 (00293770)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Tetsu 三重大学, 医学部附属病院, 講師 (20437114)
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| Co-Investigator(Renkei-kenkyūsha) |
MORSER John 三重大学, 医学系研究科, 客員教授 (40571625)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 肺気腫 / 細胞内伝達機構 / NF-kappaB / RNA干渉 / プロテアーゼ / MMP-2 / 煙草 / 炎症性サイトカイン / COPD / 難病 / 気管支炎 / タバコ煙 / マウスモデル / ヒトマトリックスメタロプロテアーゼ / プロテアーゼ阻害剤 / 慢性炎症性疾患 / 転写因子 / 核酸医薬 / サイトカイン / ケモカイン |
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| Outline of Final Research Achievements |
In the present research project, we developed a novel metalloproteinase-(MMP-)2 transgenic mouse that showed characteristic findings of chronic obstructive pulmonary disease (COPD) after short-term exposure to inhaled cigarette smoke extracts. Wild type mice usually required exposure to cigarette smoke extracts of more than half-a-year to develop COPD typical findings in the lungs. In addition, this study evaluated the therapeutic usefulness of NF-kappaB siRNA in COPD using our present mouse model. Compared to control, intranasal administration of NF-kappaB siRNA significantly suppressed lung pathological changes, cell inflammation and inflammatory cytokines. The results of this study suggest the potential therapeutic usefulness of siRNA against NF-kappaB signal pathways in COPD.
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