| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
Airway epithelial barrier dysfunction plays an important role on the pathogenesis of bronchial asthma. To identify the regulatory molecules, we used an in vitro model using airway epithelial cells. We identified NDRG1 is involved in the regulation of airway epithelial barrier. We also found that mir-155 is important for the development of epithelial barrier. PCDH1 was recently identified as a susceptibility gene in asthma. PCDH1 was upregulated with the development of epithelial barrier function in cultured airway epithelial cells. PCDH1 gene knockdown disrupted both tight and adhesion junctions. Immunohistochemical analysis revealed strong PCDH1 expression in nasal and bronchial epithelial cells; however, expression decreased in inflamed tissues sampled from patients with CRS or bronchial asthma. These results suggest that PCDH1 is important for airway function as a physical barrier, and its dysfunction is involved in the pathogenesis of allergic airway inflammation.
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