| Project/Area Number |
24591160
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
YUTARO Nakamura 浜松医科大学, 医学部附属病院, 講師 (60436962)
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| Co-Investigator(Kenkyū-buntansha) |
SUDA Takafumi 浜松医科大学, 医学部, 教授 (30291397)
NAGATA Toshi 浜松医科大学, 医学部, 教授 (90275024)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 結核 / 樹状細胞 / 細胞性免疫 / ワクチン / 結核症 |
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| Outline of Final Research Achievements |
The development of effective vaccine strategies for tuberculosis(TB) is one of the major frontiers of medical research. Our previous studies showed that type-1 polarized dendritic cell (alphaDC1) vaccine is a promising approach for eliciting protective immunity against intracellular bacteria in animal model. Here we showed that alpha DC1 from TB patients could significantly induce antigen specific type-1 immunity. Monocyte-derived DCs from TB patients were induced to mature using a "standard" cytokine cocktail or using an alpha-type 1-polarized DC (alphaDC1) cocktail and were pulsed with established TB antigen (ESAT-6). AlphaDC1 secreted substantially higher levels of IL-12p70 than standard DCs. Furthermore alphaDC1 induced much higher numbers of interferon gamma producing functional T cells against TB. The current demonstration that TB antigen pulsed alphaDC1 are potent inducers of TB-specific T cells helps to develop improved immunotherapies.
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