| Project/Area Number |
24591164
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Osaka University |
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Principal Investigator |
OKA Yoshihiro 大阪大学, 医学(系)研究科(研究院), 特任教授 (20273691)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEDA Yoshito 大阪大学, 医学(系)研究科(研究院), 助教 (40452388)
TAKAHASHI Ryo 大阪大学, 医学(系)研究科(研究院), 助教 (10546865)
NAGATOMO Izumi 大阪大学, 医学(系)研究科(研究院), 助教 (10570583)
OTSUKA Tomoyuki 大阪大学, 医学部付属病院, 特任助教 (20730576)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | WT1 / 免疫治療 / 悪性腫瘍 / T細胞レセプター / CTL / ペプチドワクチン / 癌抗原 / Th17 / 癌ワクチン / 癌免疫療法 / ヘルパーTリンパ球 / ヘルパーペプチド / 癌免疫 / ヘルパー / 胸部悪性疾患 / 免疫療法 / がんワクチン / 肺がん |
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| Outline of Final Research Achievements |
In order to molecularly analyze cancer antigen WT1-specific immune response, we cloned a gene for T cell receptor that recognizes WT1-CTL(cytotoxic T lymphocyte) peptide with the restriction of HLA class I, and two distinct genes for those that recognize WT1-helper peptide with the restriction of HLA class II, respectively. We identified WT1-specific Th17 cells with helper function. Using the method for comprehensive analysis of gene expression profiles at a single cell level, we found that there are molecularly distinct two subpopulations in effector-memory subset of WT1-specific CTLs in peripheral blood mononuclear cells of acute myeloid leukemia patients.
We successively performed WT1 peptide-based immunotherapy (WT1 peptide vaccine) for patients with malignancies, including inoperable thoracic malignant tumors.
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