Directed differentiation of collecting duct cells from human iPS cells towards the creation of novel in vitro models for cystic kidney diseases
| Project/Area Number |
24591195
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Kyoto University |
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Principal Investigator |
OSAFUNE Kenji 京都大学, iPS細胞研究所, 教授 (80502947)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 腎臓 / 尿管芽 / 中間中胚葉 / 集合管 / iPS細胞 / ES細胞 / ADPKD / ARPKD / SALL4 / OSR1 / 再生 / 分化誘導 |
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| Outline of Final Research Achievements |
The directed differentiation methods from human ES or iPS cells into renal lineage cells have not yet been established. In this study, we aimed to develop a differentiation protocol from human iPS cells into collecting duct cells in order to create disease models for autosomal dominant polycystic kidney disease (ADPKD) and autosomal recessive polycystic kidney disease (ARPKD). By using combinatorial treatments of growth factors, we partially succeeded in generating collecting duct cells from human iPS cells through intermediate mesoderm and ureteric bud. We will aim to establish an efficient differentiation method to generate collecting duct cells from human iPS cells and create novel in vitro disease models for ADPKD and ARPKD by differentiating the patient-derived iPS cells into collecting duct cells.
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Report
(4 results)
Research Products
(48 results)
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[Journal Article] 多発性嚢胞腎2015
Author(s)
松井 敏、長船 健二.
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Journal Title
遺伝子医学MOOK27「iPS細胞と難病疾患―臨床・創薬応用研究の最新知見」
Volume: なし
Pages: 198-202
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[Journal Article] Monitoring and robust induction of nephrogenic intermediate mesoder m from human pluripotent stem cells2013
Author(s)
Shin-Ichi Mae, Akemi Shono, Fumihiko Shiota, Tetsuhiko Yasuno, Masatoshi Kajiwara, Nanaka Gotoda-Nishimura, Sayaka Arai, Aiko Sato-Otubo, Taro Toyoda, Kazutoshi Takahashi, Naoki Nakayama, Chad A. Cowan, Takashi Aoi, Seishi Ogawa, Andrew P. McMahon, Shinya Yamanaka, Kenji Osafune
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Journal Title
Nature Communications
Volume: 4:1367
Issue: 1
Pages: 1367-1367
DOI
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Peer Reviewed
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[Presentation] Monitoring and robust induction of intermediate mesoderm from human iPSCs and ESCs.2012
Author(s)
Mae SI, Shono A, Shiota F, Yasuno T, Kajiwara M, Gotoda-Nishimura N, Arai S, Sato-Otubo A, Toyoda T, Takahashi K, Nakayama N, Cowan CA, Aoi T, Ogawa S, McMahon AP, Yamanaka S, Osafune K.
Organizer
10th Annual Meeting, International Society for Stem Cell Research
Place of Presentation
Pacifico Yokohama, Yokohama, Japan
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[Presentation] Development of efficient induction methods from human iPSCs/ESCs into intermediate mesoderm by using low-molecular weight compounds.2012
Author(s)
Araoka T, Toyohara T, Shiota F, Mae SI, Kurose Y, Ohta A, Yamanaka S, Osafune K.
Organizer
10th Annual Meeting, International Society for Stem Cell Research
Place of Presentation
Pacifico Yokohama, Yokohama, Japan
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