| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The directed differentiation methods from human ES or iPS cells into renal lineage cells have not yet been established. In this study, we aimed to develop a differentiation protocol from human iPS cells into collecting duct cells in order to create disease models for autosomal dominant polycystic kidney disease (ADPKD) and autosomal recessive polycystic kidney disease (ARPKD). By using combinatorial treatments of growth factors, we partially succeeded in generating collecting duct cells from human iPS cells through intermediate mesoderm and ureteric bud. We will aim to establish an efficient differentiation method to generate collecting duct cells from human iPS cells and create novel in vitro disease models for ADPKD and ARPKD by differentiating the patient-derived iPS cells into collecting duct cells.
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