| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
FcRγ chain (Fecr1g) is a common intra cellular subunit of Fc receptors, which is involved in the autoimmuno diseases. We synthesized a novel gene silencer PI polyamide targeting FcRγ chain for the immunological nephritis. In in vitro experiments, the PI polyamide targeting FcRγ chain inhibited expression of FcRγ chain mRNA and FcRγ chain promoter activity in cultured J774A.1 cells. In in vivo experiments, iv administered PI polyamide targeting FcRγ chain suppressed CD16/32 positive cells. Moreover, Syc inhibitor R406 suppressed glomerulonephritis in the anti-GBM antibody-induced nephritis and lupus nephropathy in SLE model mouse. We will further develop the PI polyamides targeting FcRγ chain as potential therapeutic agents for immunological nephritis.
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