Immunological network in ANCA associated glomerulonephritis
| Project/Area Number |
24591217
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Nippon Medical School |
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Principal Investigator |
SHIMIZU Akira 日本医科大学, 医学(系)研究科(研究院), 教授 (00256942)
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| Co-Investigator(Kenkyū-buntansha) |
NAGASAKA Shinya 日本医科大学, 医学部, 助教 (00573239)
MASUDA Yukinari 日本医科大学, 医学部, 講師 (70173755)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ANCA / ANCA関連腎炎 / ANCA関連血管炎 / 抗好中球細胞質抗体 / 半月体形成性糸球体腎炎 / 抗糸球体基底膜抗体腎炎 / サイトカイン / NETs / 壊死性半月体形成性腎炎 / 抗糸球体基底膜抗体病 / 壊死性糸球体腎炎 / 糸球体腎炎 |
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| Outline of Final Research Achievements |
In ANCA-associated vasculitis (AAV), necrotizing and crescentic glomerulonephritis (NCGN) was induced in WKY rats by immunization with human MPO (hMPO). The induced anti-hMPO Antibody (Ab) cross-reacted with rat neutrophils with activation in vitro. The crescent formation with NETs in glomeuli was enhanced by the additional injection of sub-nephritogenic anti-GBM Ab, whereas it was not significantly enhanced by the additional administration of TNF-α or G-CSF in vivo. NCGN rats with the sub-nephritogenic anti-GBM Ab showed elevated albuminuria and serum TNF-α, CXCL1 and CXCL2 levels. TNF-α, CXCL1, CXCL2 and CXCL8, which are mainly involved in neutrophil-endothelial interactions, were increased in glomeruli. Our results demonstrated that the acceleration of NCGN in AAV may be necessary not only accumulation of neutrophils in glomeruli, but also aberrant activation of neutrophils with increased glomerular expression of neutrophil activation-associated cytokines and chemokines.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Proliferative glomerulonephritis with monoclonal immunoglobulin G3κ deposits in association with parvovirus B19 infection.2012
Author(s)
Fujita E, Shimizu A, Kaneko T, Masuda Y, Ishihara C, Mii A, Higo S, Kajimoto Y, Kanzaki G, Nagasaka S, Iino Y, Katayama Y, Fukuda Y.
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Journal Title
Hum Pathol.
Volume: 43(12)
Issue: 12
Pages: 2326-33
DOI
Related Report
Peer Reviewed
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[Journal Article] Caspase-3-independent internucleosomal DNA fragmentation in ischemic acute kidney injury2012
Author(s)
Yoshida T, Shimizu A, Masuta Y, Mii A, Fjita E, Yoshizaki K, Higo S, Kanzaki G, Kajimoto Y, Takano H, Fukuda Y
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Journal Title
Nephron Exp Nephrol
Volume: 120
Issue: 3
Pages: 103-113
DOI
Related Report
Peer Reviewed
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[Journal Article] Involvement of matrix metalloproteinase-2 in the development of renal interstitial fibrosis in mouse obstructive nephropathy2012
Author(s)
Du X, Shimizu A, Masuda Y, Kuwahara N, Arai T, Kataoka M, Uchiyama M, Kaneko T, Akimoto T, Iino Y, Fukuda Y
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Journal Title
Lab Invest
Volume: 92
Issue: 8
Pages: 1149-1160
DOI
Related Report
Peer Reviewed
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[Presentation] 腎局在樹状細胞の表現型解析2014
Author(s)
永坂真也, 岩堀 徹, 肥後清一郎, 神崎 剛, 梶本雄介, 益田幸成, 清水 章
Organizer
日本腎臓学会学術総会 (第57回)
Place of Presentation
パシピィッコ横浜
Year and Date
2014-07-04 – 2014-07-06
Related Report
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