The role of Mafb in secondary hyperparathyroidism.

• Project/Area Number: 24591223
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: University of Tsukuba
• Principal Investigator: MORITO Naoki 筑波大学, 医学医療系, 講師 (70463825)
• Co-Investigator(Kenkyū-buntansha): YOH Keigyou 筑波大学, 医学医療系, 准教授 (90323302)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ２次性副甲状腺機能亢進症 / 二次性副甲状腺機能亢進症 / 転写因子 / 慢性腎臓病 / CKD-MBD

Outline of Final Research Achievements

Secondary hyperparathyroidism (HPT) is a common disorder in patients with chronic kidney disease (CKD). Recently, it was reported that the transcription factor Mafb is essential for differentiation of parathyroid gland. However, the role of Mafb in secondary hyperparathyroidism is not well known. Six-week-old WT (wild-type) and Mafb+/- mice were fed with adenine containing or normal diet. After 6 weeks of feeding, secondary hyperparathyroidism was assessed. Interestingly, secondary HPT was prevented in Mafb+/- mice. Mafb+/- mice had lower serum PTH levels and smaller parathyroid gland areas compared to their WT littermates after adenine feeding. Moreover, we found lower PTH and cyclinD2 expression in Mafb+/- parathyroid glands. These facts suggest that Mafb directly regulates the PTH and cyclinD2 expression in parathyroid glands.Mafb might play an important role in secondary HPT through PTH and cyclinD2 transcription.

Research Products

• [Journal Article] Overexpression of Mafb in podocytes protects against diabetic nephropathy. (2014)
  • Author(s): Morito N, Yoh K, Ojima M, Okamura M, Nakamura M, Hamada M, Shimohata H, Moriguchi T, Yamagata K, Takahashi S.
  • Journal Title: J Am Soc Nephrol. Volume: 25 Pages: 2546-2557
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Overexpression of Mafb in Podocytes Protects against Diabetic Nephropathy. (2014)
  • Author(s): Morito N, Yoh K, Ojima M, Okamura M, Nakamura M, Hamada M, Shimohata H, Moriguchi T, Yamagata K, Takahashi S.
  • Journal Title: J Am Soc Nephrol. Volume: 印刷中 Issue: 11 Pages: 2546-2557
  • DOI: 10.1681/asn.2013090993
  • Peer Reviewed / Open Access
• [Journal Article] Indoxyl sulfate down-regulates SLCO4C1 transporter through up-regulation of GATA3 (2014)
  • Author(s): Yu L, Moriguchi T, Souma T, Takai J, Satoh H, Morito N, Engel JD, Yamamoto M
  • Journal Title: Molecular and Cellular Biology Volume: 8 Issue: 11 Pages: 1929-1941
  • DOI: 10.1128/mcb.01659-13
  • Peer Reviewed / Open Access
• [Journal Article] O-Linked Glycosylation Determines the Nephritogenic Potential of IgA Rheumatoid Factor. (2014)
  • Author(s): Kihara M, Ito K, Nakata J, Otani M, Tran NL, Morito N, Takahashi S, Wada Y, Izui S.
  • Journal Title: J Am Soc Nephrol. Volume: Feb 7. [Epub ahead of print] Issue: 6 Pages: 1-9
  • DOI: 10.1681/asn.2013070771
  • Peer Reviewed / Open Access
• [Journal Article] Effects of irbesartan on inflammatory cytokine concentrations in patients with chronic glomerulonephritis. (2013)
  • Author(s): Tsuruoka S, Kai H, Usui J, Morito N, Saito C, Yoh K, Yamagata K.
  • Journal Title: Intern Med. Volume: 52 Pages: 303-308
  • NAID: 130003365884
  • Peer Reviewed
• [Journal Article] Amelioration of cisplatin- induced nephrotoxicity in Peroxiredoxin I- deficient mice. (2012)
  • Author(s): Kosuke Okada
  • Journal Title: Cancer Chemother Pharmacol Volume: 71 Issue: 2 Pages: 503-509
  • DOI: 10.1007/s00280-012-2046-0
  • Peer Reviewed
• [Journal Article] Nrf2 in bone marrow-derived cells positively contributes to the advanced stage of atherosclerotic plaque formation (2012)
  • Author(s): Harada N, Ito K, Hosoya T, Mimura J, Maruyama A, Noguchi N, Yagami KI, Morito N, Takahashi S, Maher JM, Yamamoto M, Itoh K
  • Journal Title: Free Radic Biol Med Volume: 53 Issue: 12 Pages: 2256-2262
  • DOI: 10.1016/j.freeradbiomed.2012.10.001
  • Peer Reviewed
• [Journal Article] Overexpression of RORγt under control of the CD2 promoter induces polyclonal plasmacytosis and autoantibody production in transgenic mice (2012)
  • Author(s): Yoh K, Morito N, Ojima M, Shibuya K, Yamashita Y, Morishima Y, Ishii Y, Kusakabe M, Nishikii H, Fujita A, Matsunaga E, Okamura M, Hamada M, Suto A, Nakajima H, Shibuya A, Yamagata K, Takahashi S
  • Journal Title: Eur J Immunol Volume: 42 Issue: 8 Pages: 1999-2009
  • DOI: 10.1002/eji.201142250
  • Peer Reviewed
• [Presentation] The Role of Mafb in secondary hyperparathyroidism due to chronic kidney disease. (2014)
  • Author(s): Naoki Morito, Keigyou Yoh, Akiko Fujita, Kunihiro Yamagata
  • Organizer: 51st ERA-EDTA Congress,
  • Place of Presentation: Amsterdam, The Netherlands
  • Year and Date: 2014-05-31 – 2014-06-03