| Project/Area Number |
24591237
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Chitose Institute of Science and Technology |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | 慢性腎臓病 / カルシウム代謝 / 赤外イメージング / 安定同位体 / 同位体顕微鏡 / 繊維化 / 異所性石灰化 / 配向性 / ラマンイメージング |
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| Outline of Final Research Achievements |
Chronic kidney disease (CKD) can lead to mineral and bone disorder, soft tissue fibrosis, and ectopic calcification. In this work, calcium metabolism and soft tissue fibrosis in rats with different severity of CKD were characterized using FTIR imaging, Raman imaging, and isotope microscopy. In addition, the new visualization methods of the pathological condition were developed. FTIR, Raman, and isotope images indicated that ectopic calcification was not necessarily developed at the early stage in the development of secondary hyperparathyroidism. Furthermore, it was found that Ca eluted from bones with CKD did not cause ectopic calcification immediately. In developing new visualization methods of the pathological condition, a method to determine fiber orientation in tissues was developed in order to characterize fibrosis, and negative effects of fixation and embedding processes were investigated.
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