Removal of albumin-bound toxin and albumin refolding by electrical dialysis and artificial chaperon
| Project/Area Number |
24591238
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Kidney internal medicine
|
|---|
| Research Institution | Kitasato University |
|---|
Principal Investigator |
KOKUBO Kenichi 北里大学, 医療衛生学部, 准教授 (20287965)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Hirosuke 北里大学, 医療衛生部, 教授 (70153632)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | アルブミン結合毒素 / 効率的除去 / インドキシル硫酸 / リフォールディング / 電気透析 / 人工シャペロン |
|---|
| Outline of Final Research Achievements |
The aim of the present study was to develop a method to effectively remove protein bound toxins by unbinding toxin from protein using denaturant and by refolding the protein. Using urea as a denaturant, protein bound toxins effectively removed. The protein can be refold by rapidly removing urea by the modified apparatus for dialysis.
|
Report
(4 results)
Research Products
(4 results)
