| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
There are patients with autoantibodies for neuronal acetylcholine receptor (nAChR alpha 3 subunit) with autonomic nervous system dysfunction. We used excessive expression cells of nAChR (alpha-3 and beta-4), and investigated the effects of patient’s serum and monoclonal anti-alpha-3 antibodies. Patient serum containing medium induced internalization of nAChR (alpha-3 and beta-4) into the cytoplasm. Monoclonal anti-alpha-3 antibodies decreased the expression of alpha-3 subunit and reduced the concentration of Ca2+ in cytoplasm. Processing at 4 °C revealed no decrease of alpha-3 subunit on the surface, that indicated endocytosis had a role in the process of decrease of alpha-3 subunits on the cell surface.
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