| Project/Area Number |
24591259
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
YAMANAKA Koji 名古屋大学, 環境医学研究所, 教授 (80446533)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 筋萎縮性側索硬化症 / マイクロアレイ / アストロサイト / ミクログリア / トランスクリプトーム / 自然免疫 / 遺伝子 / グリア |
|---|
| Outline of Final Research Achievements |
We employed unbiased screening to identify significantly misregulated 173 genes in sporadic ALS spinal cords with microarray. Similar experiments have been done before, therefore we went further by newly focusing on the cell-types that express misregulated genes. With cell-type specific transcriptome, we could analyze the microarray data from spinal cord samples composed of heterogenous cell-types in full detail. Nearly half of the misregulated genes in ALS spinal cords were expressesd abunduntly in microglia or astrocytes. Many of those genes were also changed in ALS mouse models. We confirmed that the prediction for the cell type abundantly expressing the genes were correct by the immunohistochemistry for CLEC7A, GPR65, NOX2, CHI3L1, Oncostatin M receptor, CEBPD, and calponin 3 with ALS mouse spinal cords.
We could present the useful methods for analyzing microarray data derived from spinal cord samples composed of heterogenous cell-types.
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