Functional analysis of G-protein coupled receptor 3 for future applications of novel stroke therapy utilizing neuronal stem cell transplantation
| Project/Area Number |
24591264
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Hiroshima University |
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Principal Investigator |
TANAKA Shigeru 広島大学, 医歯薬保健学研究院, 講師 (20512651)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Masayasu 広島大学, 医歯薬保健学研究院, 教授 (20192346)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | GPCR / cAMP / PKA / 神経細胞 / Neuron / ｃAMP |
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| Outline of Final Research Achievements |
G-protein coupled receptor 3 (GPR3) belongs to a member of constitutively active Gs-coupled receptors that activate 3’-5’-cyclic adenosine monophosphate (cAMP) and is highly expressed in the brain. In the current study, principal investigator has clarified that GPR3 was distributed in the cortex, striatum, hippocampus, medial habenular nucleus, and cerebellum. The GPR3 subcellular localization analysis using fluorescent-tagged GPR3 revealed that GPR3 was distributed along the plasma membrane and in goldi bodies and endosomes in neurons. Moreover, time-lapse experiments revealed that fluorescent punctae of GPR3 were transported along the neurite, which was associated with the local activation of PKA at the neurite tips.
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Report
(4 results)
Research Products
(50 results)
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[Journal Article] Effects of the Chemical Chaperone 4-Phenylbutylate on the Function of the Serotonin Transporter (SERT) Expressed in COS-7 Cells2013
Author(s)
Fujiwara, M., Yamamoto, H., Miyagi, T., Seki, T., Tanaka, S., Hide, I., Sakai, N.
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Journal Title
Journal of Pharmacological Sciences
Volume: 122
Issue: 2
Pages: 71-83
DOI
NAID
ISSN
1347-8613, 1347-8648
Related Report
Peer Reviewed
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[Journal Article] A brain-specific Grb2-associated regulator of extracellular signal-regulated kinase (Erk)/mitogen-activated protein kinase (MAPK) (GAREM) subtype, GAREM2, contributes to neurite outgrowth of neuroblastoma cells by regulating Erk signaling.2013
Author(s)
Taniguchi T, Tanaka S, Ishii A, Watanabe M, Fujitani N, Sugeo A, Gotoh S, Ohta T, Hiyoshi M, Matsuzaki H, Sakai N, Konishi H.
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Journal Title
J Biol Chem.
Volume: 288
Issue: 41
Pages: 29934-29942
DOI
Related Report
Peer Reviewed
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[Journal Article] Mutant gammaPKC that causes spinocerebellar ataxia type 14 upregulates Hsp70, which protects cells from the mutant's cytotoxicity.2013
Author(s)
Ogawa, K., Seki, T., Onji, T., Adachi, N., Tanaka, S., Hide, I., Saito, N., Sakai, N.
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 440
Issue: 1
Pages: 25-30
DOI
Related Report
Peer Reviewed
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[Journal Article] Establishment of a novel fluorescence-based method to evaluate chaperone-mediate autophagy in a singlz neuron2011
Author(s)
Seki, T., Ki Y., Tanaka, S., Dohi, E., Onji, T., Yamamoto, K., Seki T, Yoshino KI, Tanaka S, Dohi E, Onji T, Yamamoto K, Hide, I., Sakai, N. et al.
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Journal Title
PLoS ONE
Volume: 7
Issue: 2
Pages: e31232-e31232
DOI
Related Report
Peer Reviewed
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[Journal Article] Hypoxic stress activates chaperone-mediated autophagy and modulates neuronal cell survival2011
Author(s)
Dohi, E., Tanaka, S., Seki, T, m Miyagi, T., Hide, I., Sakai, N. et al.
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Journal Title
Neurochemistry International
Volume: 60
Issue: 4
Pages: 431-442
DOI
Related Report
Peer Reviewed
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