| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
G-protein coupled receptor 3 (GPR3) belongs to a member of constitutively active Gs-coupled receptors that activate 3’-5’-cyclic adenosine monophosphate (cAMP) and is highly expressed in the brain. In the current study, principal investigator has clarified that GPR3 was distributed in the cortex, striatum, hippocampus, medial habenular nucleus, and cerebellum. The GPR3 subcellular localization analysis using fluorescent-tagged GPR3 revealed that GPR3 was distributed along the plasma membrane and in goldi bodies and endosomes in neurons. Moreover, time-lapse experiments revealed that fluorescent punctae of GPR3 were transported along the neurite, which was associated with the local activation of PKA at the neurite tips.
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