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Therapeutic approach for ER stress in the pathogenesis of ALS

Research Project

Project/Area Number 24591269
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Neurology
Research InstitutionKumamoto University

Principal Investigator

YAMASHITA Satoshi  熊本大学, 生命科学研究部, 准教授 (20457592)

Co-Investigator(Renkei-kenkyūsha) ERA Takumi  熊本大学, 発生医学研究所, 教授 (00273706)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords筋萎縮性側索硬化症 / iPS細胞 / 遠位型ミオパチー / 小胞体ストレス / 運動ニューロン
Outline of Final Research Achievements

We have established iPS cell lines derived from sporadic and familial ALS (SOD1, FUS, and MATR3 mutations) patients. We induced these iPS cells into motor neurons, and examined the expression of endoplasmic reticulum (ER) stress-related molecules. In this experiment, some of ER stress-related molecules including ER chaperones, transcription factors, and pro-apoptotic factors were upregulated in the mutant FUS-expressing cells. However, a few molecules were affected uniformly in the different types of the FUS mutations. A further examination would be required to confirm the results. We also needs to consider limitations under the experiments using iPS cells, such as variability among iPS clones and developmental condition into motor neurons.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (42 results)

All 2015 2014 2013 2012 2011 Other

All Journal Article (23 results) (of which Peer Reviewed: 23 results,  Open Access: 6 results,  Acknowledgement Compliant: 2 results) Presentation (16 results) Book (3 results)

  • [Journal Article] Clinicopathological features of the first Asian family having vocal cord and pharyngeal weakness with distal myopathy due to a MATR3 mutation.2015

    • Author(s)
      Yamashita S, Mori A, Nishida Y, Kurisaki R, Tawara N, Nishikami T, Misumi Y, Ueyama H, Imamura S, Higuchi Y, Hashiguchi A, Higuchi I, Morishita S, Yoshimura J, Uchino M, Takashima H, Tsuji S, Ando Y.
    • Journal Title

      Neuropathol Appl Neurobiol

      Volume: 41 Issue: 3 Pages: 391-398

    • DOI

      10.1111/nan.12179

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Reply to comments on 'gait disturbance due to foot drop is refractory to treatment in nonsystemic vasculitic neuropathy'.2015

    • Author(s)
      Hirahara T, Yamashita S, Misumi Y, Kawakami K, Hori H, Honda S, Watanabe M, Ikeda T, Yamashita T, Maeda Y, Ando Y.
    • Journal Title

      Eur Neurol

      Volume: 73 Issue: 1-2 Pages: 80-80

    • DOI

      10.1159/000368225

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Osteopontin-integrin α(v)β(3) axis is crucial for 5-fluorouracil resistance in oral squamous cell carcinoma.2015

    • Author(s)
      Nakamura T, Shinriki S, Jono H, Ueda M, Nagata M, Guo J, Hayashi M, Yoshida R, Ota T, Ota K, Kawahara K, Nakagawa Y, Yamashita S, Nakayama H, Hiraki A, Shinohara M, Ando Y.
    • Journal Title

      FEBS Lett

      Volume: 589 Issue: 2 Pages: 231-239

    • DOI

      10.1016/j.febslet.2014.12.004

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Intrinsic TGF-β2-triggered SDF-1-CXCR4 signaling axis is crucial for drug resistance and a slow-cycling state in bone marrow-disseminated tumor cells.2015

    • Author(s)
      Nakamura T, Shinriki S, Jono H, Guo J, Ueda M, Hayashi M, Yamashita S, Zijlstra A, Nakayama H, Hiraki A, Shinohara M, Ando Y.
    • Journal Title

      Oncotarget

      Volume: 6 Pages: 1008-1019

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gait Disturbance due to Foot Drop Is Refractory to Treatment in Nonsystemic Vasculitic Neuropathy2014

    • Author(s)
      Hirahara T, Yamashita S, Misumi Y, Kawakami K, Hori H, Honda S, Watanabe M, Ikeda T, Yamashita T, Maeda Y, Ando Y
    • Journal Title

      Eur Neurol

      Volume: 71 Issue: 3-4 Pages: 180-186

    • DOI

      10.1159/000355531

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Hypoxia suppresses cylindromatosis (CYLD) expression to promote inflammation in glioblastoma: possible link to acquired resistance to anti-VEGF therapy.2014

    • Author(s)
      Guo J, Shinriki S, Su Y, Nakamura T, Hayashi M, Tsuda Y, Murakami Y, Tasaki M, Hide T, Takezaki T, Kuratsu J, Yamashita S, Ueda M, Li JD, Ando Y, Jono H.
    • Journal Title

      Oncotarget

      Volume: 5 Pages: 6353-6364

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Decision making of amyotrophic lateral sclerosis patients on noninvasive ventilation to receive tracheostomy positive pressure ventilation.2014

    • Author(s)
      Kurisaki R, Yamashita S, Sakamoto T, Maruyoshi N, Uekawa K, Uchino M, Ando Y.
    • Journal Title

      Clin Neurol Neurosurg

      Volume: 125 Pages: 28-31

    • DOI

      10.1016/j.clineuro.2014.07.008

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Clinical features of Japanese patients with inclusion body myositis.2014

    • Author(s)
      Hori H, Yamashita S, Tawara N, Hirahara T, Kawakami K, Nishikami T, Maeda Y, Ando Y.
    • Journal Title

      J Neurol Sci

      Volume: 346 Issue: 1-2 Pages: 133-137

    • DOI

      10.1016/j.jns.2014.08.009

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Concomitant accumulation of α-synuclein and TDP-43 in a patient with corticobasal degeneration.2014

    • Author(s)
      Yamashita S, Sakashita N, Yamashita T, Tawara N, Tasaki M, Kawakami K, Komohara Y, Fujiwara Y, Kamikawa M, Nakagawa T, Hirano T, Maeda Y, Hasegawa M, Takeya M, Ando Y
    • Journal Title

      Journal of Neurology

      Volume: 261 Issue: 11 Pages: 2209-2217

    • DOI

      10.1007/s00415-014-7491-8

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Drastic therapy for listerial brain abscess involving combined hyperbaric oxygen therapy and antimicrobial agents.2014

    • Author(s)
      Nakahara K, Yamashita S, Ideo K, Shindo S, Suga T, Ueda A, Honda S, Hirahara T, Watanabe M, Yamashita T, Maeda Y, Yonemochi Y, Takita T, Ando Y.
    • Journal Title

      J Clin Neurol

      Volume: 10 Issue: 4 Pages: 358-62

    • DOI

      10.3988/jcn.2014.10.4.358

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] New type of Sendai virus vector provides transgene-free iPS cells derived from chimpanzee blood.2014

    • Author(s)
      Fujie Y, Fusaki N, Katayama T, Hamasaki M, Soejima Y, Soga M, Ban H, Hasegawa M, Yamashita S, Kimura S, Suzuki S, Matsuzawa T, Akari H, Era T
    • Journal Title

      PloS one

      Volume: 9 Issue: 12 Pages: 113052-113052

    • DOI

      10.1371/journal.pone.0113052

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Stromal Expression of Neutrophil Gelatinase-Associated Lipocalin Correlates with Poor Differentiation and Adverse Prognosis in Oral Squamous Cell Carcinoma.2014

    • Author(s)
      Shinriki S., Jono H., Ueda M., Obayashi K., Nakamura T., Ota K., Ota T., Sueyoshi T., Guo J., Hayashi M., Hiraki A., Nakayama H., Yamashita S., Shinohara M., & Ando Y.
    • Journal Title

      Histopathology

      Volume: 64 Issue: 3 Pages: 353-364

    • DOI

      10.1111/his.12293

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Clinical significance of CYLD downregulation in breast cancer.2014

    • Author(s)
      Hayashi M, Jono H, Shinriki S, Nakamura T, Guo J, Sueta A, Tomiguchi M, Fujiwara S, Yamamoto-Ibusuki M, Murakami K, Yamashita S, Yamamoto Y, Li JD, Iwase H, Ando Y.
    • Journal Title

      Breast Cancer Res Treat.

      Volume: 143 Issue: 3 Pages: 447-457

    • DOI

      10.1007/s10549-013-2824-3

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Multiple system atrophy in a patient with primary ciliary dyskinesia.2013

    • Author(s)
      Sakaguchi H., Yamashita S., Suga T., Kimura E., Maeda Y., Hirano T., & Uchino M.
    • Journal Title

      Neurol Asia

      Volume: 18 Pages: 101-103

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Bilateral basal ganglia lesions as initial manifestation of CNS invasion in adult T-cell leukemia2013

    • Author(s)
      Matsuo Y, Yamashita S, Honda S, Nakajima M, Ueda A, Hirahara T, Yamashita T, Maeda Y, Hirano T, Ando Y
    • Journal Title

      J Neurol Sci

      Volume: 327(1-2) Issue: 1-2 Pages: 63-4

    • DOI

      10.1016/j.jns.2013.01.035

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Muscle biopsy findings predictive of malignancy in rare infiltrative dermatomyositis2013

    • Author(s)
      Uchino M, Yamashita S, Uchino K, Mori A, Hara A, Suga T, Hirahara T, Koide T, Kimura E, Yamashita T, Ueda A, Kurisaki R, Suzuki J, Honda S, Maeda Y, Hirano T, Ando Y
    • Journal Title

      Clin Neurol Neurosurg

      Volume: 115(5) Issue: 5 Pages: 603-6

    • DOI

      10.1016/j.clineuro.2012.07.019

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Optineurin is potentially associated with TDP-43 and involved in the pathogenesis of inclusion body myositis.2013

    • Author(s)
      Yamashita S., Kimura E., Tawara N., Sakaguchi H., Nakama T., Maeda Y., Hirano T., Uchino M., & Ando Y.
    • Journal Title

      Neuropathol. Appl. Neurobiol.

      Volume: 39 Issue: 4 Pages: 406-416

    • DOI

      10.1111/j.1365-2990.2012.01297.x

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Efficient and reproducible myogenic differentiation from human iPS cells: prospects for modeling Miyoshi Myopathy in vitro.2013

    • Author(s)
      Tanaka, A., et al
    • Journal Title

      PLoS ONE

      Volume: 8(4) Issue: 4 Pages: e61540-e61540

    • DOI

      10.1371/journal.pone.0061540

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Heatstroke in patients with Parkinson's disease2012

    • Author(s)
      Satoshi Yamashita
    • Journal Title

      Neurological Sciences

      Volume: 33 Issue: 3 Pages: 685-687

    • DOI

      10.1007/s10072-011-0842-7

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Significant CMAP decrement by repetitive nerve stimulation is more frequent in median than ulnar nerves of patients with amyotrophic lateral sclerosis2012

    • Author(s)
      Satoshi Yamashita, Hideya Sakaguchi, Akira Mori
    • Journal Title

      Muscle Nerve

      Volume: 45 Issue: 3 Pages: 426-428

    • DOI

      10.1002/mus.22301

    • NAID

      120005123115

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Long-term outcome of polymyositis treated with high single-dose alternate-day prednisolone therapy2012

    • Author(s)
      Makoto Uchino
    • Journal Title

      European Neurology

      Volume: 68 Issue: 2 Pages: 117-121

    • DOI

      10.1159/000338474

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Myasthenic crisis patients who require intensive care unit management2012

    • Author(s)
      Hideya Sakaguchi
    • Journal Title

      Muscle and Nerve

      Volume: 46 Issue: 3 Pages: 440-442

    • DOI

      10.1002/mus.23445

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Sporadic juvenile amyotrophic lateral sclerosis caused by mutant FUS/TLS : possible association of mental retardation with this mutation2011

    • Author(s)
      Satoshi Yamashita, Akira Mori, Hideya Sakaguchi
    • Journal Title

      Journal of Neurplogy

      Volume: 259 Issue: 6 Pages: 1039-1044

    • DOI

      10.1007/s00415-011-6292-6

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Analyses of the pathogenesis in muscle-specific wild-type TDP-43 transgenic mice.2014

    • Author(s)
      Satoshi Yamashita, Kensuke Kawakami, Tomo Nishikami, Nozomu Tawara, Yasushi Maeda, Yukio Ando.
    • Organizer
      19th International Congress of the World Muscle Society
    • Place of Presentation
      Langenbeck-Virchow-Haus, Berlin, Germany
    • Year and Date
      2014-10-08
    • Related Report
      2014 Annual Research Report
  • [Presentation] Cytoplasmic 5’-nucleotidase 1A are aggregated in type 2 fiber in sporadic inclusion body myositis.2014

    • Author(s)
      Nozomu Tawara, Satoshi Yamashita, Tomo Nishikami, Kensuke Kawakami, Akie Tawara, Hiroko Hori, Tomoo Hirahara, Yasushi Maeda, Yukio Ando.
    • Organizer
      19th International Congress of the World Muscle Society
    • Place of Presentation
      Langenbeck-Virchow-Haus, Berlin, Germany
    • Year and Date
      2014-10-08
    • Related Report
      2014 Annual Research Report
  • [Presentation] The first Asian family having vocal cord and pharyngeal weakness with distal myopathy due to a MATR3 mutation.2014

    • Author(s)
      Tomo Nishikami, Satoshi Yamashita, Nozomu Tawara, Akira Mori, Hiroko Hori, Tomoo Hirahara, Yasushi Maeda, Yukio Ando.
    • Organizer
      19th International Congress of the World Muscle Society
    • Place of Presentation
      Langenbeck-Virchow-Haus, Berlin, Germany
    • Year and Date
      2014-10-08
    • Related Report
      2014 Annual Research Report
  • [Presentation] 診断から18年間経過観察しえた大脳皮質基底核変性症の剖検例-αシヌクレインとTDP-43、タウ蛋白の局在について-.2014

    • Author(s)
      山下 賢、坂下直実、山下太郎、植田明彦、田崎雅義、川上賢祐、菰原義弘、藤原章雄、上川将史、中川雄伸、前田 寧、長谷川成人、竹屋元裕、安東由喜雄.
    • Organizer
      第55回日本神経病理学会総会
    • Place of Presentation
      学術総合センター、東京、日本
    • Year and Date
      2014-06-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] アジア初の声帯および咽頭麻痺を伴う遠位型ミオパチー(MPD2/ VCPDM)の1家系2症例に関する多角的検討.2014

    • Author(s)
      西上 朋、山下 賢、俵 望、森 麗、西田泰斗、内野克尚、永利聡仁、栗﨑玲一、中西俊人、堀 寛子、平原智雄、渡邉聖樹、上山秀嗣、山下太郎、前田寧、安東由喜雄.
    • Organizer
      第55回日本神経学会学術大会
    • Place of Presentation
      福岡国際会議場、福岡、日本
    • Year and Date
      2014-05-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] 孤発性封入体筋炎の自己抗原NT5C1Aはタイプ2線維に凝集している.2014

    • Author(s)
      俵 望, 山下 賢, 西上 朋, 川上 賢祐, 前田 寧, 安東 由喜雄.
    • Organizer
      第55回日本神経学会学術大会
    • Place of Presentation
      福岡国際会議場、福岡、日本
    • Year and Date
      2014-05-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] 封入体筋炎におけるCylindromatosis蛋白発現の検討.2014

    • Author(s)
      山元雅典、俵 望、山下 賢、児玉美里、中村ひとみ、西上 朋、川上賢祐、安東由喜雄.
    • Organizer
      第55回日本神経学会学術大会
    • Place of Presentation
      福岡国際会議場、福岡、日本
    • Year and Date
      2014-05-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] 筋特異的なTDP-43発現によるIBMモデルマウス作製と病態解析.2014

    • Author(s)
      山下 賢,川上賢祐, 西上 朋, 俵 望, 安東由喜雄.
    • Organizer
      第55回日本神経学会学術大会
    • Place of Presentation
      福岡国際会議場、福岡、日本
    • Year and Date
      2014-05-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] DCTN1遺伝子多型を有し、異なる神経症候を呈した1家系母子例.2014

    • Author(s)
      髙松孝太郎,山下賢,岩田有生,進藤誠悟,三隅洋平,山下太郎,安東由喜雄.
    • Organizer
      第55回日本神経学会学術大会
    • Place of Presentation
      福岡国際会議場、福岡、日本
    • Year and Date
      2014-05-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] 筋萎縮性側索硬化症(ALS)診断における反復刺激試験の有用性の検討.2014

    • Author(s)
      森 麗、山下 賢、堀 寛子、俵 明恵、中原 圭一、高松 孝太郎、三隅 洋平、安東 由喜雄.
    • Organizer
      第55回日本神経学会学術大会
    • Place of Presentation
      福岡国際会議場、福岡、日本
    • Year and Date
      2014-05-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Involvement of optineurin as well as TDP-43 in the pathogenesis of inclusion body myositis2013

    • Author(s)
      Satoshi Yamashita
    • Organizer
      18th International WMS Congress
    • Place of Presentation
      Asilomar Conference Grounds, Asilomar, California, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] FUS/TLS変異を有する弧発性若年性ALSの2例:変異蛋白の細胞内局在の検討2012

    • Author(s)
      山下 賢
    • Organizer
      第53回日本神経学会学術大会
    • Place of Presentation
      東京国際フォーラム(東京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] Sporadic juvenile amyotrophic lateral sclerosis caused by mutant FUS/TLS: Possible association of mental retardation with this mutation2012

    • Author(s)
      Satoshi Yamashita
    • Organizer
      23th international Symposium on ALS/MND
    • Place of Presentation
      Sheraton Hotel and Towers, Chicago, IL, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] Significant CMAP decrement by repetitive nerve stimulation is more frequent in median than ulnar nerves of patients with amyotrophic lateral sclerosis2012

    • Author(s)
      Akira Mori
    • Organizer
      23th international Symposium on ALS/MND
    • Place of Presentation
      Sheraton Hotel and Towers, Chicago, IL, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] Involvement of optineurin as well as TDP-43 and involved in the pathogenesis of inclusion body myositis.

    • Author(s)
      Yamashita S, Tawara N, Kawakami K, Nishikami T, Maeda Y, Ando Y.
    • Organizer
      18th International Congress of the World Muscle Society
    • Place of Presentation
      Asilomar Conference Grounds, California, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] 封入体筋炎の病態におけるTDP-43とoptineurinの重要性.

    • Author(s)
      山下 賢, 川上賢祐, 俵 望, 堀 寛子, 木村 円, 前田 寧, 安東由喜雄.
    • Organizer
      第54回日本神経学会総会
    • Place of Presentation
      東京国際フォーラム、東京
    • Related Report
      2013 Research-status Report
  • [Book] 神経内科研修ノート 肝性脳症2015

    • Author(s)
      山下賢, 安東由喜雄
    • Publisher
      診断と治療社
    • Related Report
      2014 Annual Research Report
  • [Book] 代謝性ミオパチー 薬剤による筋障害(薬剤性ミオパチー)2014

    • Author(s)
      山下賢
    • Publisher
      診断と治療社
    • Related Report
      2014 Annual Research Report
  • [Book] 筋疾患診療ハンドブック2013

    • Author(s)
      山下賢,森麗.
    • Total Pages
      206
    • Publisher
      中外医学社
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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