| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
HTLV-1-infected CD4+ T cells and HTLV-1-specific cytotoxic T lymphocytes (CTLs) accumulate in the spinal cords of HAM/TSP patients. It is suggested that the both cells induce HTLV-1-specific inflammation, causing HAM/TSP. We attempted to develop an animal model of the disease. We established an HTLV-1-infected CD4+ T cell line and CD8+ CTL lines specific for either HTLV-1 Tax11-19 or Tax301-309 from an HLA-A2+A24+ HAM/TSP patient. NOD/SCID/JAK3-deficient mouse were inoculated with these cells and were divided into three groups; mouse with the infected cells, mouse with the infected cells and Tax11-19 CTLs, and mouse with the infected cells and Tax301-309 CTLs. However, no mouse showed neurological symptoms. Further consideration of inoculation cell number and its route would be needed.
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