The production of newly ALS model mice
Project/Area Number |
24591275
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Neurology
|
Research Institution | Kitasato University |
Principal Investigator |
NAGAI MAKIKO 北里大学, 医学部, 講師 (80420488)
|
Research Collaborator |
NIHIRA Tomoko
KAWANAMI Aya
|
Project Period (FY) |
2012-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 筋萎縮性側索硬化症 / TDP-43 / アデノ随伴ウィルス / マウスモデル / 脊髄前角細胞 / トランスジェニックマウス / アデノ随伴ウイルス / 動物モデル / optineurin / TDP-43 / Thy-1プロモーター / 神経変性 / 脊髄 / 運動神経 / Thy-1 / VachT-Cre |
Outline of Final Research Achievements |
TDP-43 was identified as the major protein component of the aggregations in the affected neurons of amyotrophic lateral sclerosis (ALS) patients.We generated the transgenic mice with loxP-dsRed-loxP-TDP-43 under the control of Thy-1.This mouse expressed dsRed in neurons of brain and spinal cord.Then we crossed this mouse with VAcht-Cre mouse, which expressed Cre in motor neurons. We expected the motor neuron deaths in the spinal cords of these double transgenic mice to use as the ALS model. These double transgenic mice did not expressed the paralysis, because the insufficiency of TDP-43 protein level. We used adeno-associated virus (AAV) vectors to overexpress the TDP-43 in the mice motor neurons. TDP-43-WT and TDP-43-A315T mice showed muscle weakness of the left hindlimb from 2 weeks of injection. We may use these mice as ALS model.
|
Report
(5 results)
Research Products
(13 results)
-
-
[Journal Article] Loss of DARPP-32 and calbindin in multiple system atrophy2013
Author(s)
Hayakawa H, Nagai M, Kawanami A, Nakata Y, Nihira T, Ogino M, Takada M, Saido T, Takano J, Saegusa M, Mikami T, Hamada J, Nishiyama K, Mochizuki H, Mizuno Y
-
Journal Title
Journal of Neural Transmission
Volume: 120
Issue: 12
Pages: 1689-1698
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Accumulation of a-synuclein triggered by presynaptic dysfunction2012
Author(s)
Nakata Y, Yasuda T, Fukaya M, Yamamori S, Itakura M, Nihira T, Hayakawa H, Kawanami A, Kataoka M, Nagai M, Sakagami H, Takahashi M, Mizuno Y, Mochizuki H
-
Journal Title
J. Neurosci
Volume: 32
Issue: 48
Pages: 17186-17196
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-