| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Outline of Final Research Achievements |
TDP-43 was identified as the major protein component of the aggregations in the affected neurons of amyotrophic lateral sclerosis (ALS) patients.We generated the transgenic mice with loxP-dsRed-loxP-TDP-43 under the control of Thy-1.This mouse expressed dsRed in neurons of brain and spinal cord.Then we crossed this mouse with VAcht-Cre mouse, which expressed Cre in motor neurons. We expected the motor neuron deaths in the spinal cords of these double transgenic mice to use as the ALS model. These double transgenic mice did not expressed the paralysis, because the insufficiency of TDP-43 protein level.
We used adeno-associated virus (AAV) vectors to overexpress the TDP-43 in the mice motor neurons. TDP-43-WT and TDP-43-A315T mice showed muscle weakness of the left hindlimb from 2 weeks of injection. We may use these mice as ALS model.
|
