| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
In the present study, we addressed whether or not the sweet taste receptor (STR) expressed in β-cells modulates metabolism of glucose. To this end, we monitored changes in intracellular ATP ([ATP]c) in MIN6 cells expressing luciferase. High concentrations of glucose induced biphasic increase in [ATP]c, which was inhibited by an addition of mannoheptulose or dinitrophenol. In the presence of 5.5 mM glucose, addition of sucralose, an activator of STR, induced an immediate and marked elevation of [ATP]c. Sucralose potentiated the effect of glucose and also augmented methylsuccinate-induced elevation of [ATP]c.
In the presence of 5.5 mM glucose, addition of 3-O-methylglucose (MeGlc), a non-metabolizable analogue of glucose, induced a transient increase in [ATP]c, which corresponded to the first peak of the glucose-induced elevation of [ATP]c. or dinitrophenol.These results indicate that activation of STR primes the metabolic pathway and enhances ATP production.
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