Development of slowly progressive type 1 diabetes animal models using the CD28 knock-out NOD mouse
Project/Area Number |
24591319
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Metabolomics
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Research Institution | Okinaka Memorial Institute for Medical Research (2014) University of Yamanashi (2012-2013) |
Principal Investigator |
KOBAYASHI Tetsuro 公益財団法人冲中記念成人病研究所, その他部局等, 研究員 (30113442)
|
Co-Investigator(Kenkyū-buntansha) |
AIDA Kaoru 山梨大学, 総合研究部, 准教授 (50184015)
ICHIJO Masashi 山梨大学, 総合研究部, 助教 (50436854)
TAKIZAWA Souichi 山梨大学, 総合研究部, 助教 (80456467)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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Keywords | 糖尿病 / 緩徐1型糖尿病 / NODマウス / CD28 / 緩徐進行1型糖尿病 / 1型糖尿病 / Type 1 diabetes / Innate immunity / ER stress |
Outline of Final Research Achievements |
The characteristic pathological features of autopsied pancreas of SPIDDM were CD8+ T cell and CD68+ macropthages to the atrophied exocrine pancreas. Sixty percent of SPIDDM pancreas had pancreatic ductal hyperplasia/metaplasia with obstructive pancreatitis (p<0.01 vs. non-diabetic controls). To establish an animal model of SPIDDM, the effect of poly I:C multiple intraperitoneal injection (poly I:C model) or intra-pancreatic ductal injection of Zein-oleic acid-linoleic acids (ZOAL) (ZOAL model) to CD28 knock-out NOD mice was examined. ZOAL model showed marked pancreatic exocrine inflammation composed of CD8+ and CD68+ cells and high occurrence of diabetes, while poly I:C model showed slight exocrine pancreatic inflammation without enhanced occurrence of diabetes. Conclusion: We could established ZOAL NOD mice model, which resembles with human SPIDDM.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Distinct cell clusters touching islet cells induce islet cell replication in association with over-expression of regenerating gene (REG) protein in fulminant type 1 diabetes.2014
Author(s)
Aida K, Saitoh S, Nishida Y, Yokota S, Ohno S, Mao X, Akiyama D, Tanaka S, Awata T, Shimada A, Oikawa Y, Shimura H, Furuya F, Takizawa S, Ichijo M, Ichijo S, Itakura J, Fujii H, Hashiguchi A, Takasawa S, Endo T, Kobayashi T
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Journal Title
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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