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Role of the microtubule-associated protein in insulin-dependent glucose uptake

Research Project

Project/Area Number 24591333
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Metabolomics
Research InstitutionThe University of Tokushima

Principal Investigator

SAKAUE Hiroshi  徳島大学, ヘルスバイオサイエンス研究部, 教授 (60372645)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
KeywordsAdipocyte / Obesity / Diabetes / 肥満 / 糖尿病 / 脂肪細胞 / 微小管
Outline of Final Research Achievements

We identified adipocyte-specific tubulin binding protein (synuclein) in WAT of C57BL/6 mice during the development of obesity. The aim of this study is to reveal the role of synuclein in tubulin regulation in adipose tissue function. Immunofluorescence staining demonstrated that synuclein can co-localized with tubulin and enhance the staining of tubulin in 3T3-L1 adipocytes. We next examine the effect of synuclein knockdown in insulin-dependent glucose uptake and triacylglycerol (TG) accumulation in 3T3-L1 adipocytes. synuclein knockdown decreased glucose uptake, TG accumulation and GLUT4 vesicles with plasma membrane in 3T3-L1 adipocytes. Synuclein knockout suprressed body weight gain for long term maintenance on a high-fat diet and enhance insulin sensitivity in mice. These observations suggest that synuclein can affect glucose uptake and lipid accumulation in adipose tissue by regulating tubulin polymerization.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (29 results)

All 2015 2014 2013 2012

All Journal Article (12 results) (of which Peer Reviewed: 11 results,  Open Access: 4 results) Presentation (12 results) (of which Invited: 1 results) Book (5 results)

  • [Journal Article] Sudachitin, a polymethoxylated flavone, improves glucose and lipid metabolism by increasing mitochondrial biogenesis in skeletal muscle.2014

    • Author(s)
      Tsutsumi R, Yoshida T, Nii Y, Okahisa N, Iwata S, Tsukayama M, Hashimoto R, Taniguchi Y, Sakaue H, Hosaka T, Shuto E, Sakai T.
    • Journal Title

      Nutr Metab

      Volume: 11 Issue: 1 Pages: 32-32

    • DOI

      10.1186/1743-7075-11-32

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Enhancement of endothelial function inhibits left atrial thrombi development in an animal model of spontaneous left atrial thrombosis.2014

    • Author(s)
      Mawatari K, Yoshioka E, Toda S, Yasui S, Furukawa H, Shimohata T, Ohnishi T, Morishima M, Harada N, Takahashi A, Sakaue H, Nakaya Y.
    • Journal Title

      Circ J.

      Volume: 78 Pages: 1980-1988

    • NAID

      130004427936

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Deletion of Hypoxia-Inducible Factor-1α in Adipocytes Enhances Glucagon-Like Peptide-1 Secretion and Reduces Adipose Tissue Inflammation2014

    • Author(s)
      Yoshitaka KIHIRA, Mariko MIYAKE, Manami HIRATA, Yoji HOSHINA, Kana KATO, Hitoshi SHIRAKAWA, Hiroshi SAKAUE, Noriko YAMANO, Yuki IZAWA-ISHIZAWA, Keisuke ISHIZAWA, Yasumasa IKEDA, Koichiro TSUCHIYA, Toshiaki TAMAKI, Shuhei TOMITA
    • Journal Title

      PLOS ONE

      Volume: 9 Issue: 4 Pages: e93856-e93856

    • DOI

      10.1371/journal.pone.0093856

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Albumin-normalized serum zinc: a clinically useful parameter for detecting taste impairment in patients undergoing dialysis.2014

    • Author(s)
      Tsutsumi R, Ohashi K, Tsutsumi YM, Horikawa YT, Minakuchi J, Minami S, Harada N, Sakaue H, Sakai T, Nakaya Y.
    • Journal Title

      Nutr Res.

      Volume: 34 Issue: 1 Pages: 11-16

    • DOI

      10.1016/j.nutres.2013.10.009

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cysteine string protein 1 (CSP1) modulates insulin sensitivity by attenuating glucose transporter 4 (GLUT4) vesicle docking with the plasma membrane.2014

    • Author(s)
      Jambaldorj B, Terada E, Hosaka T, Kishuku Y, Tomioka Y, Iwashima K, Hirata Y, Teshigawara K, Thi Kim Le C, Nakagawa T, Harada N, Sakai T, Sakaue H, Matsumoto T, Funaki M, Takahashi A, Nakaya Y.
    • Journal Title

      J Med Invest.

      Volume: 60 Pages: 197-204

    • NAID

      130004822683

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Exendin-4, a glucagon-like peptide-1 receptor agonist, attenuates neointimal hyperplasia after vascular injury2013

    • Author(s)
      Y. Hirata, H. Kurobe, C. Nishio, K. Tanaka, D. Fukuda, E. Uematsu, S. Nishimoto, T. Soeki, N. Harada, H. Sakaue, T. Kitagawa, M. Shimabukuro, N. Nakaya, M. Sata
    • Journal Title

      Eur. J. Pharmacol.

      Volume: 699 Issue: 1-3 Pages: 106-111

    • DOI

      10.1016/j.ejphar.2012.11.057

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Telmisartan ameliorates insulin sensitivity by activating the AMPK/SIRT1 pathway in skeletal muscle of obese db/db mice2012

    • Author(s)
      Shiota A, Shimabukuro M, Fukuda D, Soeki T, Sato H, Uematsu E, Hirata Y, Kurobe H, Maeda N, Sakaue H, Masuzaki H, Shimomura I, Sata M.
    • Journal Title

      Cardiovascular Diabetol.

      Volume: 11 Issue: 1 Pages: 139-139

    • DOI

      10.1186/1475-2840-11-139

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Proliferative and antiapoptotic signaling stimulated by nuclear-localized PDK1 results in oncogenesis.2012

    • Author(s)
      Kikani CK, et al.
    • Journal Title

      Sci Signal

      Volume: 5 Issue: 249 Pages: 249-249

    • DOI

      10.1126/scisignal.2003065

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Activation of AMPK-Sirt1 pathway by telmisartan in white adipose tissue: A possible link to anti-metabolic effects2012

    • Author(s)
      Shiota A, Shimabukuro M, Fukuda D, Soeki T, Sato H, Uematsu E, Hirata Y, Kurobe H, Sakaue H, Nakaya Y, Masuzaki H, Sata M
    • Journal Title

      Eur J Pharmacol

      Volume: 692 Issue: 1-3 Pages: 84-90

    • DOI

      10.1016/j.ejphar.2012.07.026

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification and functional characterization of human glycerol-3-phosphate acyltransferase 1 gene promoters.2012

    • Author(s)
      Nagakatsu Harada et al.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 423 Issue: 1 Pages: 128-133

    • DOI

      10.1016/j.bbrc.2012.05.094

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ablationof 3-phosphoinositide-dependent protein kinase 1(PDK1) in vascular endothelial cells enhances insulin sensitivity by reducing visceral fat and suppressing angiogenesis2012

    • Author(s)
      Tawaramoto K, Sakaue H, et al
    • Journal Title

      Mol Endocrinol

      Volume: Vol.26 Issue: 1 Pages: 95-109

    • DOI

      10.1210/me.2010-0412

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 【エネルギー消費と代謝障害】 運動とエネルギー消費2012

    • Author(s)
      阪上 浩
    • Journal Title

      糖尿病

      Volume: 55 Pages: 306-308

    • Related Report
      2012 Research-status Report
  • [Presentation] TBP, Adipocyte-specific Tubulin-Binding Protein, Can Control Cytoskeletal Structure, and Insulin Stimulated Glucose Uptake.2015

    • Author(s)
      Masashi Kuroda and Hiroshi Sakaue
    • Organizer
      75th Scientific Session of American diabetes association.
    • Place of Presentation
      ボストンコンベンションセンター(Boston), USA
    • Year and Date
      2015-06-05 – 2015-06-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] 細胞骨格制御分子γ-synucleinによる糖取り込み機構の解析2014

    • Author(s)
      黒田雅士、阪上浩
    • Organizer
      第35回日本肥満学会
    • Place of Presentation
      シーガイヤコンベンションセンター(福岡県福岡市)、日本
    • Year and Date
      2014-10-24 – 2014-10-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] Role of the new microtubule-associated protein in the development of obesity2013

    • Author(s)
      Okitsu R, Sakaue H
    • Organizer
      国際肥満学会
    • Place of Presentation
      Kuala Lumpur, Malaysia(Convention Centre)
    • Related Report
      2013 Research-status Report
  • [Presentation] Role of DNA methylation for Leptin Gene Promoter in 3T3-L1 Adipocytes2013

    • Author(s)
      Kuroda M, Sakaue H
    • Organizer
      国際肥満学会
    • Place of Presentation
      Kuala Lumpur, Malaysia(Convention Centre)
    • Related Report
      2013 Research-status Report
  • [Presentation] Role of DNA methylation for Leptin Gene Promoter in 3T3-L1 Adipocytes2013

    • Author(s)
      Kuroda M, Sakaue H
    • Organizer
      米国糖尿病学会
    • Place of Presentation
      Cicago, USA(McCormick Place)
    • Related Report
      2013 Research-status Report
  • [Presentation] 細胞骨格制御分子による糖取り込み機構の解析2013

    • Author(s)
      興津 理絵, 阪上 浩
    • Organizer
      日本肥満学会
    • Place of Presentation
      東京都(国際フォーラム)
    • Related Report
      2013 Research-status Report
  • [Presentation] 細胞骨格制御分子による糖取り込み機構の解析2013

    • Author(s)
      興津 理絵, 阪上 浩
    • Organizer
      日本糖尿病学会
    • Place of Presentation
      熊本(ホテル日航熊本)
    • Related Report
      2013 Research-status Report
  • [Presentation] レプチン発現制御機構を検討し得る培養脂肪細胞の作製2012

    • Author(s)
      福田 亜希子, 他
    • Organizer
      日本肥満学会
    • Place of Presentation
      京都国際会館(京都府)
    • Related Report
      2012 Research-status Report
  • [Presentation] PGC1α新規アイソフォームの肥満病態形成における役割2012

    • Author(s)
      野村 和弘, 他
    • Organizer
      日本肥満学会
    • Place of Presentation
      京都国際会館(京都府)
    • Related Report
      2012 Research-status Report
  • [Presentation] 中枢神経系におけるグレリンの摂食と運動調節経路の解析2012

    • Author(s)
      二見 明香理, 他
    • Organizer
      日本肥満学会
    • Place of Presentation
      京都国際会館(京都府)
    • Related Report
      2012 Research-status Report
  • [Presentation] Adipocyte turnover and its proliferation2012

    • Author(s)
      阪上 浩
    • Organizer
      国際肥満シンポジウム
    • Place of Presentation
      京都国際会館(京都府)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 培養脂肪細胞でのレプチン発現はDNA脱メチル化と転写因子による活性化の2つのステップで制御されている2012

    • Author(s)
      福田 亜希子, 他
    • Organizer
      日本栄養・食糧学会大会
    • Place of Presentation
      東北大学(宮城県)
    • Related Report
      2012 Research-status Report
  • [Book] 肥満と肥満症基礎研究の進歩 脂肪細胞の肥大と増殖2014

    • Author(s)
      黒田雅士、阪上浩
    • Total Pages
      728
    • Publisher
      日本臨床社
    • Related Report
      2014 Annual Research Report
  • [Book] Diabetes Strategy2013

    • Author(s)
      黒田 雅士、阪上 浩
    • Publisher
      先端医学社
    • Related Report
      2013 Research-status Report
  • [Book] Diabetes Strategy2013

    • Author(s)
      阪上 浩
    • Total Pages
      19
    • Publisher
      先端医学社
    • Related Report
      2013 Research-status Report
  • [Book] 細胞工学2013

    • Author(s)
      黒田 雅士、阪上 浩
    • Total Pages
      5
    • Publisher
      学研メディカル秀潤社
    • Related Report
      2013 Research-status Report
  • [Book] 救急・集中治療2012

    • Author(s)
      黒田 雅士, 他
    • Total Pages
      2
    • Publisher
      総合医学社
    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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