Elucidation of the role of molecular motor proteins in the mechanism of action of steroid hormone receptor

• Project/Area Number: 24591367
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Endocrinology
• Research Institution: Kyushu University
• Principal Investigator: KAWATE HISAYA 九州大学, 大学病院, 助教 (20336027)
• Co-Investigator(Renkei-kenkyūsha): TAKAYANAGI Ryoichi 九州大学, 大学院医学研究院, 教授 (30154917)
• Research Collaborator: HORIUCHI Toshihiro
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ステロイドホルモン / 核内受容体

Outline of Final Research Achievements

1. Molecular motor protein myosins (myosin V, VI, VIII) and dynein were fused with fluorescent GFP proteins and intracellular localization of these fusion proteins were examined. However, colocalization of these molecular motor proteins with steroid hormone receptors has not been observed.
2. A new SERM, bazedoxifene, did not cause translocation of estrogen receptors into the nucleoli, which was observed after treatment with raloxifene. However, bazedoxifene markedly suppressed ER-mediated transcriptional activation as well as raloxifene.
3. It has been suggested that TXNIP is involved in the process of acquisition of glucocortocoid-induced insulin resistance in preadipocytes.

Research Products

• [Journal Article] Complete remission of anaplastic thyroid carcinoma after concomitant treatment with docetaxel and radiotherapy. (2015)
  • Author(s): Abe I, Karasaki S, Matsuda Y, Sakamoto S, Nakashima T, Yamamoto H, Kawate H, Ohnaka K, Nakashima H, Kobayashi K, Oda Y, Nomura M, Takayanagi R.
  • Journal Title: Case Rep Endocrinol Volume: 2015 Pages: 726085-726085
  • DOI: 10.1155/2015/726085
  • Peer Reviewed / Open Access
• [Journal Article] Perioperative sequential monitoring of hemodynamic parameters in patients with pheochromocytoma using the Non-Invasive Cardiac System (NICaS) (2014)
  • Author(s): Matsuda Y, Kawate H, Shimada S, Matsuzaki C, Nagata H, Adachi M, Ohnaka K, Nomura M, Takayanagi R.
  • Journal Title: Endocrine Journal Volume: 61 Issue: 6 Pages: 571-575
  • DOI: 10.1507/endocrj.EJ13-0471
  • NAID: 130004770449
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Long-term study of subclinical Cushing’s syndrome shows high prevalence of extra-adrenal malignancy in patients with functioning bilateral adrenal tumors (2014)
  • Author(s): Kawate H, Kohno M, Matsuda Y, Akehi Y, Tanabe M, Horiuchi T, Ohnaka K, Nomura M, Yanase T, Takayanagi R.
  • Journal Title: Endocrine Journal Volume: 61 Issue: 12 Pages: 1205-1212
  • DOI: 10.1507/endocrj.EJ14-0155
  • NAID: 130004687426
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] ステロイド療法を理解するための基礎知識 (2014)
  • Author(s): 高柳涼一、河手久弥
  • Journal Title: 臨床と研究 Volume: 91 Pages: 453-458
  • Open Access
• [Journal Article] 副腎皮質機能低下を早期診断・治療するために (2014)
  • Author(s): 河手久弥、髙栁涼一
  • Journal Title: 日本内科学会雑誌 Volume: 103 Pages: 878-885
  • Open Access
• [Journal Article] Proposed diagnostic criteria for subclinical Cushing’s syndrome associated with adrenal incidentaloma (2013)
  • Author(s): Akehi Y, Kawate H, Murase K, Nagaishi R, Nomiyama T, Nomura M, Takayanagi R, Yanase T.
  • Journal Title: Endocrine Journal Volume: 60 Issue: 7 Pages: 903-912
  • DOI: 10.1507/endocrj.EJ12-0458
  • NAID: 10031195759
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed
• [Journal Article] Behavioral and clinical correlates of serum bilirubin concentrations in Japanese men and women (2013)
  • Author(s): Tanaka M, Budhathoki S, Hirata A, Morita M, Kono S, Adachi M, Kawate H, Ohnaka K, Takayanagi R
  • Journal Title: BMC Endocr Disord Volume: 13(1) Issue: 1 Pages: 39-39
  • DOI: 10.1186/1472-6823-13-39
  • Peer Reviewed
• [Journal Article] Eldecalcitol for the treatment of osteoporosis. (2013)
  • Author(s): Noguchi Y, Kawate H, Nomura M, Takayanagi R.
  • Journal Title: Clin Interv Aging Volume: 8 Pages: 1313-1321
  • DOI: 10.2147/cia.s49825
  • Peer Reviewed
• [Journal Article] A Potential Improvement of Rheumatoid Arthritis in a Patient Treated with Liraglutide for Glucocorticoid-induced Diabetes (2013)
  • Author(s): 河野 倫子, 野村 政壽, 阿部 一朗, 松村 祐介, 松田 やよい, 足立 雅広, 河手 久弥, 大中 佳三, 堀内 孝彦, 高柳 涼一
  • Journal Title: Journal of the Japan Diabetes Society Volume: 56 Issue: 3 Pages: 179-184
  • DOI: 10.11213/tonyobyo.56.179
  • NAID: 10031184488
  • ISSN: 0021-437X, 1881-588X
• [Journal Article] Reduced arterial stiffness in patients with acromegaly: non-invasive assessment by the cardio-ankle vascular index (CAVI) (2013)
  • Author(s): Matsuda Y, Kawate H, Matsuzaki C, Sakamoto R, Abe I, Shibue K, Kohno M, Adachi M, Ohnaka K, Nomura M, Takayanagi R.
  • Journal Title: Endocrine Journal Volume: 60 Issue: 1 Pages: 29-36
  • DOI: 10.1507/endocrj.EJ12-0189
  • NAID: 10031156719
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed
• [Journal Article] Pheochromocytoma crisis caused by Campylobacter fetus. (2012)
  • Author(s): Abe I, Nomura M, Watanabe M, Shimada S, Kohno M, Matsuda Y, Adachi M, Kawate H, Ohnaka K, Takayanagi R.
  • Journal Title: Int J Urol Volume: 19 Issue: 5 Pages: 465-467
  • DOI: 10.1111/j.1442-2042.2011.02950.x
  • NAID: 10030754996
  • Peer Reviewed
• [Presentation] 高齢骨粗鬆症・骨折の予防と管理 (2015)
  • Author(s): 河手久弥
  • Organizer: 第25回日本老年医学会九州地方会
  • Place of Presentation: 福岡
  • Year and Date: 2015-03-07
  • Invited
• [Presentation] サブクリニカルクッシング症候群患者における動脈硬化と骨代謝の関連性 (2014)
  • Author(s): 河手 久弥, 武市 幸奈, 松田 やよい, 松村 祐介, 蘆田 健二, 大中 佳三, 野村 政壽, 髙栁 涼一
  • Organizer: 第56回日本老年医学会学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2014-06-12 – 2014-06-14
• [Presentation] バセドウ病眼症患者における肝炎ウイルスマーカー陽性率の検討 (2014)
  • Author(s): 河手 久弥(九州大学 大学院医学研究院病態制御内科学), 坂本 竜一, 坂本 絵里子, 萬代 幸子, 岡村 建, 松田 やよい, 阿部 一朗, 大中 佳三, 野村 政壽, 高柳 涼一
  • Organizer: 第87回日本内分泌学会学術総会
  • Place of Presentation: 福岡
  • Year and Date: 2014-04-24 – 2014-04-26
• [Presentation] 褐色細胞腫患者の循環血液量評価におけるNICaSの有用性の検討 (2013)
  • Author(s): 嶋田 伸吾, 松崎 千登勢, 松田 やよい, 永田 宙生, 足立 雅広, 河手 久弥, 大中 佳三, 野村 政壽, 高柳 涼一
  • Organizer: 第86回日本内分泌学会学術総会
  • Place of Presentation: 仙台
• [Presentation] エストロゲン受容体の細胞内動態と転写活性化能に対する各種SERMの影響 (2013)
  • Author(s): 堀内 俊博, 河手 久弥, 具島 三佳, 足立 雅広, 大中 佳三, 野村 政壽, 高柳 涼一
  • Organizer: 第86回日本内分泌学会学術総会
  • Place of Presentation: 仙台
• [Presentation] 原発性アルドステロン症患者の治療前後における動脈硬化指標及び代謝系の経時的評価 (2013)
  • Author(s): 松田 やよい, 松崎 千登勢, 河手 久弥, 嶋田 伸吾, 永田 宙生, 足立 雅広, 大中 佳三, 野村 政壽, 高柳 涼一
  • Organizer: 第86回日本内分泌学会学術総会
  • Place of Presentation: 仙台
• [Presentation] 高齢の副腎性サブクリニカルクッシング症候群患者における合併症の経過 (2013)
  • Author(s): 河野 倫子, 河手 久弥, 足立 雅広, 大中 佳三, 野村 政壽, 高柳 涼一
  • Organizer: 第55回日本老年医学会学術集会
  • Place of Presentation: 大阪
• [Presentation] The effects of various SERMs on the intracellular localization and the transactivation of estrogen receptor alpha (2013)
  • Author(s): Toshihiro Horiuchi, Hisaya Kawate,, Mika Gushima, Masahiro Adachi, Keizo Ohnaka, Masatoshi Nomura, Ryoichi Takayanagi
  • Organizer: The Endocrine Society's 95th Annual Meeting
  • Place of Presentation: San Francisco, USA
• [Presentation] 副腎サブクリニカルクッシング症候群患者の長期予後調査 (2012)
  • Author(s): 河野倫子、河手久弥、阿部一朗、松田やよい、足立雅広、大中佳三、明比祐子、柳瀬敏彦、野村政壽、髙栁涼一
  • Organizer: 第20回日本ステロイドホルモン学会学術集会
  • Place of Presentation: 金沢
• [Presentation] Long term follow-up of patients with adrenal subclinical Cushing syndrome (2012)
  • Author(s): Hisaya Kawate, Michiko Kohno, Ichiro Abe, Yayoi Matsuda, Masahiro Adachi, Keizo Ohnaka, Yuko Akehi, Toshihiko Yanase, Masatoshi Nomura, Ryoichi Takayanagi
  • Organizer: 15th International Congress on Hormonal Steroids and Hormones & Cancer
  • Place of Presentation: Kanazawa
• [Presentation] 前立腺癌におけるアクチビン受容体の活性化とリンパ節転移 (2012)
  • Author(s): 野村 政壽, 田中 公貴, 足立 雅広, 河手 久弥, 高柳 涼一
  • Organizer: 第20回日本ステロイドホルモン学会学術集会
  • Place of Presentation: 金沢
• [Presentation] 天然型ビタミンDが耐糖能に及ぼす影響とその機序に関する検討 (2012)
  • Author(s): 川原 佑太, Enciso PL, 河手 久弥, 足立 雅広, 野村 政壽, 高柳 涼一
  • Organizer: 第20回日本ステロイドホルモン学会学術集会
  • Place of Presentation: 金沢