The mechanism of ectopic PTH-, but not PTHrP-,production in retroperitoneal tumor
| Project/Area Number |
24591372
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Endocrinology
|
|---|
| Research Institution | Teikyo University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | PTH / 異所性ホルモン産生腫瘍 / 次世代シークエンス法 / マイクロアレイ / CYP24A1 / ATRX / 異所性ホルモン発現腫瘍 / エキソームシークエンシング / 副甲状腺 / ビタミンD / HIC1 / PTHrP / 組織特異的遺伝子発現 |
|---|
| Outline of Final Research Achievements |
We confirmed intratumoral overproduction of PTH, but not PTHrP, in a retroperitoneal malignant fibrous hyperplasia in a patient who showed lethal hypercalcemia by using RT-PCR and immunohistochemical assay. The rearrangements or mutations of the PTH gene were not observed, while several transcriptional factors responsible for PTH gene regulation were markedly affected in their amounts. Our RNA-seq revealed that copy number variation of chromosome 11 where PTH gene resides occurred much more frequently than that in other chromosomes. Further, we found nonsense mutations of ATRX, med1 and USP24 and so on. Lastly, we found that CYP24A1 mRNA was drastically upregulated, suggesting that resultant intratumoral hypovitaminosis D might lead to uncontrollable intratumoral overproduction of PTH protein. Additional oncogenic stimuli such as perturbations of ATRX or med1/USP24 may well resulted in ectopic PTH overproduction.
|
Report
(4 results)
Research Products
(11 results)
-
-
-
-
-
-
[Presentation] Aberrant, but physiological, interactions between the non-mutated androgen receptor and E2 in several sex hormone-responsive cancer cells2014
Author(s)
Takao Susa, Reina Ikaga, Takashi Kajitani, Masayoshi Iizuka, Mimi Tamamori-Adachi, Tomoki Okazaki
Organizer
ENDO2014 MEETING and EXPO, June 21-24, 2014, Chicago
Place of Presentation
シカゴ
Year and Date
2014-06-21 – 2014-06-24
Related Report
-
-
-
-
-
