| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
The identification and/or functional analysis of bioactive peptide provide novel insight into the regulatory mechanisms of physiological phenomena.
In this study, the regional distribution of a neuropeptide neuromedin S (NMS) in rat brain at the peptide level was determined by the radioimmunoassay. A high content of its peptide was found in the hypothalamus and brainstem, whereas NMS mRNA was abundantly expressed only in the hypothalamus. These data suggest that nerve fibers containing NMS peptide produced by hypothalamic neuron project into the brainstem. On the other hand, a novel bioactive peptide synthesized from a precursor protein of known neuropeptide was identified by in silico screening. A 37-residues novel peptide was isolated from AtT20 cells overexpressing the precursor protein, and was detected immunologically in the extract of rat brain. Intracerebroventricular administration of this peptide to rats induced increase in plasma concentration of specific pituitary hormone.
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