Genetic and epigenetic abnormalities in malignant lymphoma.
Project/Area Number |
24591388
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Hematology
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Research Institution | Nagoya University |
Principal Investigator |
TOMITA AKIHIRO 名古屋大学, 医学(系)研究科(研究院), 准教授 (80378215)
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Co-Investigator(Renkei-kenkyūsha) |
KIYOI Hitoshi 名古屋大学, 大学院医学系研究科, 教授 (90314004)
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Research Collaborator |
SUZUKI Yasuhiro 国立病院機構, 名古屋医療センター, 血液内科
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 悪性リンパ腫 / 遺伝子異常 / 網羅的解析 / エピゲノム / 末梢血無細胞遊離核酸 / 網羅的遺伝子変異解析 / 末梢血遊離DNA / 遺伝子変異 / ヒストン修飾 |
Outline of Final Research Achievements |
To confirm the epigenetic abnormalities in malignant lymphoma cells, protein expression of methylated histone and histone metyltransferases were confirmed by using whole cell lysate of tumor biopsy cells. The expression level was differ in each sample, suggesting that some genetic abnormalities in genes encoding epigenetic factors may be lying. To perform genetic analyses including epigenetic factors related genes in the most of lymphoma patients, comprehensive genetic analyses strategies using peripheral blood cell-free DNA were established. PB-cfDNA concentration reflects the disease status in diffuse large B-cell lymphoma patients, and it is suggested that PB-cfDNA can be used for genetic analyses using samples harvested serially during disease progression.
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Report
(4 results)
Research Products
(63 results)
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[Journal Article] Target antigen density governs the efficacy of anti-CD20-CD28-CD3 ζ chimeric antigen receptor-modified effector CD8+ T cells.2015
Author(s)
Watanabe K, Terakura S, Martens AC, van Meerten T, Uchiyama S, Imai M, Sakemura R, Goto T, Hanajiri R, Imahashi N, Shimada K, Tomita A, Kiyoi H, Nishida T, Naoe T, Murata M.
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Journal Title
J Immunol.
Volume: 194(3)
Issue: 3
Pages: 911-20
DOI
Related Report
Peer Reviewed
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[Journal Article] Prognostic significance of pleural or pericardial effusion and the implication of optimal treatment in primary mediastinal large B-cell lymphoma: a multicenter retrospective study in Japan.2014
Author(s)
Aoki T, Izutsu K, Suzuki R, Nakaseko C, Arima H, Shimada K, Tomita A, Sasaki M, Takizawa J, Mitani K, Igarashi T, Maeda Y, Fukuhara N, Ishida F, Niitsu N, Ohmachi K, Takasaki H, Nakamura N, Kinoshita T, Nakamura S, Ogura M.
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Journal Title
Haematologica
Volume: 99(12)
Issue: 12
Pages: 1817-25
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] De novo diffuse large B-cell lymphoma with a CD20 immunohistochemistry-positive and flow cytometry-negative phenotype: Molecular mechanisms and correlation with rituximab sensitivity.2014
Author(s)
Tokunaga T, Tomita A, Sugimoto K, Shimada K, Iriyama C, Hirose T, Shirahata-Adachi M, Suzuki Y, Mizuno H, Kiyoi H, Asano N, Nakamura S, Kinoshita T, Naoe T.
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Journal Title
Cancer Sci.
Volume: 105
Issue: 1
Pages: 35-43
DOI
Related Report
Peer Reviewed
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[Journal Article] Epstein-Barr virus-positive cytotoxic T-cell lymphoma followed by chronic active Epstein-Barr virus infection-associated T/NK-cell lymphoproliferative disorder: a case report2013
Author(s)
Kato S, Miyata T, Takata K, Shimada S, Ito Y, Tomita A, Elsayed A, Takahashi E, Asano N, Kinoshita T, Kimura H, Nakamura S.
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Journal Title
Human Pathology
Volume: 44
Issue: 12
Pages: 2849-2852
DOI
Related Report
Peer Reviewed
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[Journal Article] Escape of leukemia blasts from HLA-specific CTL pressure in a recipient of HLA one locus-mismatched bone marrow transplantation.2012
Author(s)
Kato T, Terakura S, Murata M, Sugimoto K, Murase M, Iriyama C, Tomita A, Abe A, Suzuki M, Nishida T, Naoe T.
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Journal Title
Cellular Immunology
Volume: 276
Issue: 1-2
Pages: 75-82
DOI
Related Report
Peer Reviewed
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[Presentation] Development and Analysis of Novel Intravascular Large B-Cell Lymphoma NOD/Shi-Scid IL2Rγnull Mouse Xenograft Model.2014
Author(s)
Kazuyuki Shimada, Satoko Shimada, Keiki Sugimoto, Fumihiko Hayakawa, Miyuki Katayama, Akihiro Hirakawa, Yusuke Takagi, Shigeo Nakamura, Masao Seto, Tomoki Naoe, Akihiro Tomita and Hitoshi Kiyoi.
Organizer
The American Society of Hematology, 56th Annual Meeting. (oral presentation)
Place of Presentation
San Francisco, CA, USA
Year and Date
2014-12-08
Related Report
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[Presentation] Clinical and Molecular Significance of Peripheral Blood Cell-Free DNA in B-Cell Lymphomas for Detection of Genetic Mutations and Correlation with Disease Status.2014
Author(s)
Yasuhiro Suzuki, Akihiro Tomita, Kenichi Yoshida, Kazuyuki Shimada, Chisako Iriyama, Masashi Sanada, Yuichi Shiraishi, Kenichi Chiba, Hiroko Tanaka, Satoru Miyano, Seishi Ogawa, and Hitoshi Kiyoi.
Organizer
The American Society of Hematology, 56th Annual Meeting.
Place of Presentation
San Francisco, CA, USA
Year and Date
2014-12-06
Related Report
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[Presentation] Rituximab Sensitivity to De Novo DLBCL Cells Showing the Specific Phenotype of CD20 Protein Immunohistochemistry-Positive / Flow Cytometory-Negative: Analyses of Its Clinical Significances and the Molecular Mechanisms.2012
Author(s)
Akihiro Tomita, Takashi Tokunaga, Tatsuya Hirose, Keiki Sugimoto, Kazuyuki Shimada, Norio Kaneda, Hitoshi Kiyoi, Naoko Asano, Shigeo Nakamura, Tomohiro Kinoshita, and Tomoki Naoe.
Organizer
The American Society of Hematology, 54th Annual Meeting
Place of Presentation
Georgia World Congress Center Atlanta, GA, USA
Related Report
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[Presentation] Usefulness of Peripheral Blood Circulating DNAs As an Alternative to DNA From Bone Marrow Cells to Detect CpG Global Methylation Status and Genetic Mutations in Patients with Myelodysplastic Syndromes.2012
Author(s)
Chisako Iriyama, Akihiro Tomita, Hideaki Hoshino, Mizuho Shirahata, Yoko Hibi, Kiyofumi Yamada, Hitoshi Kiyoi, and Tomoki Naoe.
Organizer
The American Society of Hematology, 54th Annual Meeting
Place of Presentation
Georgia World Congress Center Atlanta, Atlanta, GA, USA
Related Report
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