Grant-in-Aid for Scientific Research (C)
Abnormal epigenetic regulations have been shown to be involved in the pathogenesis and/or progression of myelodysplastic syndrome (MDS) and this characteristic of disease led to the introduction of DNA demethylating agents including Azacytidine and Aza-deoxycytidine (Aza-dC) for the clinical management of MDS. In this study, we characterized genes whose expressions are directly increased by Aza-dC using K562 cells, in which DNA demethylation induces hemoglobin (Hb) synthesis, as an experimental model. By analyzing changes in DNA methylation status and gene expression together with histone modification, we found that genes that are suppressed by promoter methylation but are ready to be transcribed will be activated by DNA demethylation. In addition, we elucidated a mechanism by which K562 cells produce Hb upon DNA demethylation.
Nucleic Acids Research
Nucleic Acid Res.
Antimicrobial Agents Chemotherapy
Attribution of KAKENHI