Identification and functional analysis of ATL cancer stem cells using a humanized mice
| Project/Area Number |
24591414
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
HAMAGUCHI Isao 国立感染症研究所, その他部局等, その他 (90348780)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | HTLV-1感染モデル / ヒト化マウス / ATL幹細胞 / HTLV-1 / モデルマウス |
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| Outline of Final Research Achievements |
At the first step, we developed humanized mice by using NOJ (NOD/SCID/JAK3 KO) mouse. After CD34 positive hematopoietic stem cells were transplanted into a neonatal liver of NOJ mice, in the course of 10 weeks 30-70% human CD45 cells had been revealed. At the point of 16 weeks, enough amount of CD4 positive T cells existed. For the HTLV-1 infection, MMC treated MT-2 cells (2.5x106) were inoculated into the humanized mouse peritoneum. HTLV-1 infected T cells were increased in the mouse liver, spleen, bone marrow. These data suggested that there might be a ATL stem cell niche in the spleen. Moreover we analyzed the efficacy of new drug candidate of anti-HTLV-1 infection by using with this mouse model. This drug led to the suppression of proviral road of HTLV-1 in the peripheral blood and of infected T cell growth in the mouse model. These data suggested that the mouse model would be an effective tool for the new drug candidate.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] Identification of TL-Om1, an ATL Cell Line, as a Reference Material for Human T-Lymphotropic Virus 1 Quantitative PCR.2015
Author(s)
Kuramitsu M, Okuma K, Yamagishi M, Yamochi T, Firouzi S, Momose H, Mizukami T, Takizawa K, Araki K, Sugamura K, Yamaguchi K, Watanabe T, Hamaguchi I.
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Journal Title
Journal of Clinical Microbiology
Volume: 53
Issue: 2
Pages: 587-596
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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