Development of novel differentiation induction system of hematopoietic stem cells from human induced pluripotent stem cells
| Project/Area Number |
24591415
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Tokyo Metropolitan Institute of Medical Science |
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Principal Investigator |
KITAJIMA Kenji 公益財団法人東京都医学総合研究所, 生体分子先端研究分野, 主席研究員 (10346132)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | iPS細胞 / 造血幹細胞 / ホメオボックス / 細胞分化 / 試験管内分化誘導 / 転写因子 / 再生医療 / 血液細胞 |
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| Outline of Final Research Achievements |
We previously demonstrated that hematopoietic stem cell (HSC)-like cells were obtained from mouse induced pluripotent stem cells (iPSCs). When mouse iPSCs are differentiated into hemogenic endothelial cells (HECs) and a transcription factor Lhx2, known to induce ex vivo amplification of adult HSCs, is introduced, HSC-like cells are differentiated. Based on this finding, we challenged to induced HSCs from human iPSCs. First, we established an efficient in vitro hematopoietic differentiation induction system from human iPSCs. We found that a GSK3beta inhibitor greatly improved hematopoietic differentiation from human iPSCs. Using this method, we carried out enforced expression of Lhx2 into human iPSC-derived HECs. However, HSC-like cells were hardly obtained.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Endothelial cell-specific expression of roundabout 4 is regulated by differential DNA methylation of the proximal promoter.2014
Author(s)
Okada Y, Funahashi N, Tanaka T, Nishiyama Y, Yuan L, Shirakura K, Turjman AS, Kano Y, Naruse H, Suzuki A, Sakai M, Zhixia J, Kitajima K, Ishimoto K, Hino N, Kondoh M, Mukai Y, Nakagawa S, Garcia-Cardena G, Aird WC, Doi T.
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Journal Title
Arterioscler Thromb Vasc Biol
Volume: 34
Pages: 1531-1538
Related Report
Peer Reviewed
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[Journal Article] Embryonic stem cell differentiation system for evaluating gene functions involved in physiological megakaryocytic differentiation.2012
Author(s)
Okada Y, Yonekura M, Watanabe M, Nakai T, Wakimura A, Shimizu M, Kamikawa Y, Kitayama M, Kitajima K, Aird WC, Doi T.
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Journal Title
Biochem Biophys Res Commun
Volume: 419
Pages: 477-481
Related Report
Peer Reviewed
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