The recognition and signaling pathway of cysteine protease activity in innate immune cells
| Project/Area Number |
24591419
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Shinshu University |
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Principal Investigator |
HIDA Shigeaki 信州大学, 学術研究院医学系, 准教授 (10345762)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | シグナル伝達 / 好塩基球 / サイトカイン / アレルギー / プロテアーゼ |
|---|
| Outline of Final Research Achievements |
Basophils have recently been recognized as an initiator of type 2 immune responses producing interleukin-4 (IL-4) in response to various stimuli. The cysteine protease papain was shown to induce production of IL-4 and other molecules in murine basophils. Papain has been shown to not only trigger IL-4 production but also activate antigen presentation machinery by basophils, thereby initiating Th2 responses. However, the papain receptor and the signaling pathway for IL-4 production induced by the protease remained unclear. Here we show that basophils lacking FcRγ failed to produce IL-4 in response to papain. Retroviral reconstitution experiments showed that the FcRγ-ITAM-Syk signaling pathway was essential in papain-induced IL-4 production. In addition, papain specifically induced the expression of IFN-related genes in bone marrow-derived basophils. These observations indicated that other receptors as well as FcRγ-associated molecules could potentially recognize the protease papain.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] NFkB2 gene as anoble candidate epidemiologically responding to interval walking training.2015
Author(s)
Zhang Y, Hashimoto S, Fujii C, Hida S, Ito K, Matsumura T, Sakaizawa T, Morikawa M, Masuki S, Nose H, Higuchi K, Nakajima K, Taniguchi S
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Journal Title
Int. J. Sports. Med.
Volume: in press
Issue: 09
Pages: 769-775
DOI
Related Report
Peer Reviewed / Open Access
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