Basic research for hematopoietic stem cell transplantation before birth
Project/Area Number |
24591429
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Hematology
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Research Institution | Jichi Medical University |
Principal Investigator |
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Research Collaborator |
NAGAO Yosikazu 宇都宮大学, 農学部, 教授 (70291953)
ABE Tomoyuki 自治医科大学, 医学部 分子病態治療研究センター, 助教 (20610364)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | 造血幹細胞移植 / 出生前治療 / 先天性免疫不全症 / 大型動物 / ヒツジ / 子宮内移植 / 幹細胞移植 / 胎児 / 前処置 |
Outline of Final Research Achievements |
X-linked severe combined immunodeficiency has been successfully treated in US and European countries by in utero transplantation (IUT) of hematopoietic stem cells; however, the engraftment efficiency has not necessarily been high enough for other diseases in which graft cells do not have growth advantage. From a series of experiments in the setting of sheep IUT, we have shown that the engraftment efficiency can be improved by the following methods: (1) Pre-conditioning of fetuses with busulfan, (2) transient forced expression of the HoxB4 gene in donor hematopoietic stem cells, and (3) post-conditioning with cytokines.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Generating porcine chimeras using inner cell mass cells and parthenogenetic preimplantation embryos2013
Author(s)
Nakano, K., Watanabe, M., Matsunari, H., Matsuda, T., Honda, K., Maehara, M., Kanai, T., Hayashida, G., Kobayashi, M., Kuramoto, M., Arai, Y., Umeyama, K., Fujishiro, S.-H., Mizukami, Y., Nagaya, M., Hanazono, Y., Nagashima, H.
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Journal Title
PLOS ONE
Volume: 8
Pages: -
DOI
Related Report
Peer Reviewed
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[Journal Article] DNA methylation profiles provide a viable index for porcine pluripotent stem cells2013
Author(s)
Arai, Y., Ohgane, J., Fujishiro, S.-H., Nakano, K., Matsunari, H., Watanabe, M., Umeyama, K., Azuma, D., Uchida, N., Sakamoto, N., Makino, T., Yagi, S., Shiota, K., Hanazono, Y., Nagashima, H.
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Journal Title
Genesis
Volume: 51
Pages: 763-776
DOI
Related Report
Peer Reviewed
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[Journal Article] Generation of interleukin-2 receptor gamma gene knockout pigs from somatic cells genetically modified by zinc finger nuclease-encoding mRNA2013
Author(s)
Watanabe, M., Nakano, K., Matsunari, H., Matsuda, T., Maehara, M., Kanai, T., Kobayashi, M., Matsumura, Y., Sakai, R., Kuramoto, M., Hayashida, G., Asano, Y., Takayanagi, S., Arai, Y., Umeyama, K., Nagaya, M., Hanazono, Y., Nagashima, H.
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Journal Title
PLOS ONE
Volume: 8
Pages: -
DOI
Related Report
Peer Reviewed
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[Journal Article] Maternal administration of busulfan before in utero transplantation of human hematopoietic stem cells enhances engraftments in sheep2012
Author(s)
Abe, T., Masuda, S., Tanaka, Y., Nitta, S., Kitano, Y., Hayashi, S., Hanazono, Y., Nagao, Y.
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Journal Title
Experimental Hematology
Volume: 40
Pages: 436-444
DOI
Related Report
Peer Reviewed
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[Presentation] Evaluation of porcine induced pluripotent stem cells based on the DNA methylation profile of mouse embryonic stem cell-specific hypomethylated loci2013
Author(s)
Arai, Y., Ohgane, J., Fujishiro, S., Nakano, K., Matsunari, H., Watanabe, M., Umeyama, K., Azuma, D., Uchida, N., Sakamoto, N., Makino, T., Yagi, S., Shiota, K., Hanazono, Y., Nagashima, H.
Organizer
第36回日本分子生物学会
Place of Presentation
神戸
Year and Date
20131203-20131206
Related Report
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[Presentation] Factors influencing engraftment of monkey embryonic stem cells in sheep after xenogeneic in utero transplantation2013
Author(s)
Nagao, Y., Abe, T., Tanaka, Y., Sasaki, K., Masuda, S., Sarentonglaga, B., Ogata, K., Yamaguchi, M., Hayashi, S., Kitano, Y., Hanazono, Y.
Organizer
12th Congress of International Xenotransplantation Association
Place of Presentation
大阪
Year and Date
20131110-20131113
Related Report
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[Presentation] Long-term comparative study on the engraftment of human hematopoietic stem cells in sheep after xenogeneic in utero transplantation2013
Author(s)
Abe, T., Masuda, S., Sarentonglaga, B., Ogata, K., Yamaguchi, M., Hayashi, S., Nagao, Y., Hanazono, Y.
Organizer
12th Congress of International Xenotransplantation Association
Place of Presentation
大阪
Year and Date
20131110-20131113
Related Report
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[Presentation] Long-term follow-up on the engraftment of human HSCs in sheep after in utero transplantation2012
Author(s)
Hanazono, Y., Abe, T., Nitta, S., Masuda, S., Hayashi, S., Nagao, Y.
Organizer
International Society for Hematology and Stem Cells 41st Annual Scientific Meeting
Place of Presentation
Amsterdam, Netherland
Year and Date
20120823-20120826
Related Report
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[Presentation] Possible factors for engraftment of monkey embryonic stem cells after in utero transplantation into sheep fetuses2012
Author(s)
Nagao, Y., Abe, T., Tanaka, Y., Sasaki, K., Masuda, S., Nitta, S., Sarentonglaga, B., Hayashi, S., Kitano, Y., Hanazono, Y.
Organizer
17th International Congress on Animal Reproduction
Place of Presentation
Vancouver, Canada
Year and Date
20120729-20120802
Related Report
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[Presentation] HoxB4-transduction of human HSC results in long-term engraftment compared to busulfan-conditioning in sheep after in utero transplantation2012
Author(s)
Abe, T., Nitta, S., Masuda, S., Hayashi, S., Nagao, Y., Hanazono, Y.
Organizer
The 18th Annual Meeting of Japan Society of Gene Therapy
Place of Presentation
熊本
Year and Date
20120628-20120630
Related Report
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[Presentation] Long-term comparative study on the engraftment of human hematopoietic stem cells in sheep2012
Author(s)
Abe, T., Nitta, S., Masuda, S., Hayashi, S., Nagao, Y., Hanazono, Y
Organizer
International Society for Stem Cell Research 10th Annual Meeting
Place of Presentation
横浜
Year and Date
20120613-20120616
Related Report
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[Presentation] HoxB4-transduction of human HSC results in longer-term engraftment compared to busulfan-conditioning in sheep after in utero transplantation2012
Author(s)
Abe, T., Nitta, S., Masuda, S., Hayashi, S., Nagao, Y., Hanazono, Y.
Organizer
第10回 幹細胞シンポジウム
Place of Presentation
淡路島
Year and Date
20120531-20120602
Related Report
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