Characterization of human CD34-negative hematopoietic stem cells and their differentiation pathway and hierarchy
| Project/Area Number |
24591432
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Hematology
|
|---|
| Research Institution | Kansai Medical University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SASAKI Yutaka 関西医科大学, 医学部, 准教授 (80425066)
MATSUOKA Yoshikazu 関西医科大学, 医学部, 助教 (70533420)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 造血幹細胞 / 臍帯血 / CD34抗原 / CD133抗原 / 骨髄内移植 / SCID-repopulating cell / 階層制 / 臍帯血移植 / HSC / SRC / CD34 / CD133 / MSC / hierarchy / cord blood / HSCT / IBMI / MPL |
|---|
| Outline of Final Research Achievements |
We have identified human cord blood-derived very primitive CD34-negative hematopoietic stem cell (CD34-HSC) using the intra-bone marrow injection method for the first time. Recently, we developed a high resolution purification method for CD34-HSC using 18 lineage specific antibodies and succeeded to identify CD133 antigen as a specific positive marker for CD34+/- HSCs. Furthermore, we performed a variety of in vitro and in vivo experiments to clarify the human HSC hierarchy. These data demonstrated that our identified CD34-HSC seemed to be more immature than the CD34+CD38-HSC whidh was previously thought as the most primitive human HSC.
Based on these data, we propose that the myeloid-biased long-term repopulating CD34-HSCs reside in the apex of human HSC hierarchy.
|
Report
(4 results)
Research Products
(46 results)
-
[Journal Article] Prospectively isolated human bone marrow cell-derived MSCs support primitive human CD34-negative hematopoietic stem cells2015
Author(s)
Matsuoka Y, Nakatsuka R, Sumide K, Kawamura H, Takahashi M, Fujioka T, Uemura Y, Asano H, Sasaki Y, Inoue M, Ogawa H, Takahashi T, Hino M, Sonoda Y
-
Journal Title
Stem Cells
Volume: 33
Issue: 5
Pages: 1554-1565
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] CD133 is a positive marker of human cord blood-derived CD34-negative hematopoietic stem cells.
Author(s)
Takahashi M, Matsuoka Y, Sumida K, Nakatsuka R, Fujioka T, Kohno H, Sasaki Y, Matsui K, Asano H, Kaneko K, Sonoda Y
Organizer
The 55th Annual Meeting and Exposition of American Society of Hematology
Place of Presentation
New Orleans (USA)
Related Report
-
-
-
-
-
-
-
-
-
[Presentation] Functional significance of MPL expression in the human primitive hematopoietic stem cell compartment.
Author(s)
Takahashi M, Matsuoka Y, Iwaki R, Nakatsuka R, Fujioka T, Kohno H, Matsui K, Asano H, Sasaki Y, Kaneko K, Sonoda Y.
Organizer
The 54th Annual Meeting of the American Society of Hematology
Place of Presentation
Atlanta (USA)
Related Report
-
-
-
-
-
-
-
-
-
-
