Identification of novel therapeutic targets for rheumatoid arthritis by genome-wide analysis of gene expression in peripheral CD4 positive T cells
Project/Area Number |
24591441
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
膠原病・アレルギー・感染症内科学
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Research Institution | Chiba University |
Principal Investigator |
IKEDA Kei 千葉大学, 医学部附属病院, 助教 (10456014)
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Co-Investigator(Kenkyū-buntansha) |
NAKAJIMA Hiroshi 千葉大学, 医学部附属病院, 教授 (00322024)
TAKATORI Hiroaki 千葉大学, 医学部附属病院, 助教 (30568225)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 関節リウマチ / IL-6 / ヘルパーT細胞 / ARID5A / Helios / Bcl-3 / Th17細胞 / CD4陽性T細胞 / トシリズマブ / DNAマイクロアレイ / DNAアレイ / RORgt |
Outline of Final Research Achievements |
We analyzed the comprehensive gene expression in peripheral CD4 positive T cells from patients with rheumatoid arthritis (RA) who achieved a good response after treatment with tocillizumab. We focused on ARID5A, Helios, and Bcl-3, the expression of which significantly decreased after treatment and further investigated the mechanisms. We demonstrated that ARID5A is a novel molecule which inhibits Th17 differentiation probably through direct binding to RORgt. We also demonstrated that Helios potentiates the function of regulatory T cells cooperatively with FoxP3. We also demonstrated that Bcl-3 is involved in follicular helper T cell differentiation and the pathophysiology of RA.
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Report
(4 results)
Research Products
(7 results)
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[Journal Article] Prediction of therapeutic responses to tocilizumab in patients with rheumatoid arthritis – biomarkers identified by analyses of gene expression in peripheral blood mononuclear cells using genome-wide DNA microarray2014
Author(s)
Sanayama Y, Ikeda K, Saito Y, Kagami S, Yamagata M, Furuta S, Kashiwakuma D, Iwamoto I, Umibe T, Nawata Y, Matsumura R, Sugiyama T, Sueishi M, Hiraguri M, Nonaka K, Ohara O, Nakajima H
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Journal Title
Arthritis Rheumatol
Volume: in press
Issue: 6
Pages: 1421-1431
DOI
Related Report
Peer Reviewed
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[Journal Article] AT-rich interactive domain-containing protein 5a functions as a negative regulator of RORγt-induced Th17 cell differentiation.2014
Author(s)
Saito Y, Kagami SI, Sanayama Y, Ikeda K, Suto A, Kashiwakuma D, Furuta S, Iwamoto I, Nonaka K, Ohara O, Nakajima H.
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Journal Title
Arthritis and Rheumatology
Volume: 印刷中
Issue: 5
Pages: 1185-94
DOI
Related Report
Peer Reviewed
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[Presentation] Prediction of therapeutic responses to tocilizumab in patients with rheumatoid arthritis using biomarkers identified by genome-wide DNA microarray analysis in peripheral blood mononuclear cells2014
Author(s)
Sanayama Y, Ikeda K, Saito Y, Kagami S, Yamagata M, Furuta S, Kashiwakuma D, Iwamoto I, Umibe T, Nawata Y, Matsumura R, Sugiyama T, Sueishi M, Hiraguri M, Nonaka K, Ohara O, Nakajima H
Organizer
2014 European Colleague Against Rheumatism Congress
Place of Presentation
Paris, France
Year and Date
2014-06-13
Related Report