| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
We comprehensively analyzed the gene expression in synoviocytes before and after adipogenesis. As a result, we found that MMP-1 and MMP-2 transcripts are down-regulated in synoviocyte after adipogenesis induction. The protein expressions of MMP-1 and MMP-2 are also determined. We expected that mesenchymal phenotype is diminished in synoviocyte by adipogenesis induction. As we expected, the mRNA and protein expressions of smooth muscle actin, one of mesenchymal markers, are reduced by adipogenesis induction. We found Artepillin-C, a natural PPAR ligand derived from propolis is a potent adipogenesis inducer. Consistent with the previous data, MMP-1 and MMP-2 are reduced in synoviocyte after adipogenesis that is induced by Artepillin-C. These findings suggested that adipogenesis induction of synoviocytes by using Artepillin-C contribute to the treatment of rheumatoid arthritis.
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