Analyses of gene expressions related with rheumatoid arthritis and epithelial mesenchymal transition in fibroblast-like synoviocyte by adipogenesis.
| Project/Area Number |
24591448
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
膠原病・アレルギー・感染症内科学
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YAMASAKI Satoshi 広島大学, 大学病院, 病院助教 (30367388)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 関節リウマチ / 滑膜細胞 / 脂肪分化 / 間葉系幹細胞 / 上皮間葉移行 / サイトカイン / プロテアーゼ / PPAR / ＰＰＡＲ |
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| Outline of Final Research Achievements |
We comprehensively analyzed the gene expression in synoviocytes before and after adipogenesis. As a result, we found that MMP-1 and MMP-2 transcripts are down-regulated in synoviocyte after adipogenesis induction. The protein expressions of MMP-1 and MMP-2 are also determined. We expected that mesenchymal phenotype is diminished in synoviocyte by adipogenesis induction. As we expected, the mRNA and protein expressions of smooth muscle actin, one of mesenchymal markers, are reduced by adipogenesis induction. We found Artepillin-C, a natural PPAR ligand derived from propolis is a potent adipogenesis inducer. Consistent with the previous data, MMP-1 and MMP-2 are reduced in synoviocyte after adipogenesis that is induced by Artepillin-C. These findings suggested that adipogenesis induction of synoviocytes by using Artepillin-C contribute to the treatment of rheumatoid arthritis.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Significant improvement in MRI-proven bone edema is associated with protection from structural damage in very early RA patients managed using the tight control approach.2012
Author(s)
Kita J, Tamai M, Arima K, Kawashiri SY, Horai Y, Iwamoto N, Okada A, Koga T, Nakashima Y, Suzuki T, Yamasaki S, Nakamura H, Origuchi T, Ida H, Aoyagi K, Uetani M, Eguchi K, Kawakami A
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Journal Title
Mod Rheumatol
Volume: 23
Issue: 2
Pages: 254-259
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Musculoskeletal ultrasonography assists the diagnostic performance of the 2010 classification criteria for rheumatoid arthritis.2012
Author(s)
Kawashiri SY, Suzuki T, Okada A, Yamasaki S, Tamai M, Nakamura H, Origuchi T, Mizokami A, Uetani M, Aoyagi K, Eguchi K, Kawakami A
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Journal Title
Mod Rheumatol
Volume: 23
Issue: 1
Pages: 36-43
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] The diagnostic utility of anti-melanoma differentiation-associated gene 5 antibody testing for predicting the prognosis of Japanese patients with DM.2012
Author(s)
Koga T, Fujikawa K, Horai Y, Okada A, Kawashiri SY, Iwamoto N, Suzuki T, Nakashima Y, Tamai M, Arima K, Yamasaki S, et al.
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Journal Title
Rheumatology (Oxford)
Volume: 51
Issue: 7
Pages: 1278-84
DOI
NAID
Related Report
Peer Reviewed
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