EBV induced erosive arthritis in humanized NOG mouse
| Project/Area Number |
24591457
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
膠原病・アレルギー・感染症内科学
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| Research Institution | Nihon University |
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Principal Investigator |
TAKEI Masami 日本大学, 医学部, 教授 (30179600)
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| Co-Investigator(Kenkyū-buntansha) |
SHIRAIWA Hidetaka 日本大学, 医学部, 助教 (20451338)
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| Co-Investigator(Renkei-kenkyūsha) |
KITAMURA Noboru 日本大学, 医学部, 准教授 (40349999)
IWATA Mitsuhiko 日本大学, 医学部, 兼任講師 (50193751)
IKUMI Natsumi 日本大学, 医学部, 専修医 (50727857)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 関節リウマチ / EBウイルス / びらん性関節炎 / 破骨細胞 / NOGマウス / ヒト化マウス / Epstein-Barrウイルス(EBV) / ビラン性関節炎 / 骨改造 / ヒト免疫化マウス（NOG) / 生物学的製剤 / Epstein-Barrウイルス（EBV) / ヒト免疫化マウス / Epstein-Barrウイルス / ヒト免役化マウス / 治療実験 |
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| Outline of Final Research Achievements |
NOG mice are injected with human CD34+ stem cells from cord blood (LONZA) intravenously. Erosive arthritis was observed histologically at the high rate of almost 100%. Human cathepsin K stain and TRAP of multinuclear cells that exist in bone erosion were positive. Multinucleated giant cells resemble osteoclasts are observed in cultured bone marrow cells with human RANKL and M-CSF. Human cathepsin K, mitochondria stain and TRAP were all positive in these multinucleated cells.
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Report
(4 results)
Research Products
(8 results)
