Integrons as epidemiological molecular markers for identifying and surveying multi-drug resistance genes in Gram-negative bacilli
| Project/Area Number |
24591489
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Infectious disease medicine
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| Research Institution | Showa University |
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Principal Investigator |
ZHAO Wei-Hua 昭和大学, 医学部, 講師 (90327916)
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| Co-Investigator(Kenkyū-buntansha) |
HU Zhi-Qing 昭和大学, 医学部, 准教授 (60245826)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | グラム陰性桿菌 / 薬剤耐性遺伝子 / インテグロン / β-ラクタマーゼ / メタロ-β-ラクタマーゼ (MBL) / PCR分析 / DNAシークエンス解析 / 薬剤耐性グラム陰性菌 / 分子疫学 / 耐性遺伝子 / インテグロン-1 / β-ラクタム剤 / メタロ-β-ラクタマーゼ |
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| Outline of Final Research Achievements |
We collected 80 clinical isolates of metallo-β-lactamases (MBL)-producing Gram-negative bacilli from several hospitals and extracted their DNA. A pair of primers was designed to target the 5' and 3’ conserved segments of class 1 integron and used in PCR amplification of the potential resistance determinants harbored by the bacteria. The DNA sequencing data of PCR products revealed that 5 types of metallo-β-lactamases including blaIMP-1,-7. -10, -11 and blaVIM-2 coexisted with resistance determinants encoding aminoglycoside-modifying enzymes in the class 1 integrons. The diversity of gene cassette arrangement in a class 1 integron implicates different origins and acquisition events of the resistance determinants. The results indicate that the experimental design is reasonable and integron 1 can be used as an epidemiological molecular marker for efficiently identifying and surveilling MBL-producers. The related papers are published in medical journals.
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Report
(4 results)
Research Products
(14 results)
