| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
We collected 80 clinical isolates of metallo-β-lactamases (MBL)-producing Gram-negative bacilli from several hospitals and extracted their DNA. A pair of primers was designed to target the 5' and 3’ conserved segments of class 1 integron and used in PCR amplification of the potential resistance determinants harbored by the bacteria. The DNA sequencing data of PCR products revealed that 5 types of metallo-β-lactamases including blaIMP-1,-7. -10, -11 and blaVIM-2 coexisted with resistance determinants encoding aminoglycoside-modifying enzymes in the class 1 integrons. The diversity of gene cassette arrangement in a class 1 integron implicates different origins and acquisition events of the resistance determinants. The results indicate that the experimental design is reasonable and integron 1 can be used as an epidemiological molecular marker for efficiently identifying and surveilling MBL-producers. The related papers are published in medical journals.
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